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Vol. 37 No. 11 (2025): Vol 37 Issue 11, 2025

Copyright (c) 2025 sangeeta Gupta, Amanpreet Kaur, Neha Bhatt, Manvir Singh, Shubhangee Agarwal

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Green Synthesis of Silver Nanoparticles using Raphanus sativus Water Extract for Enhanced Antimicrobial and Photocatalytic Activities

https://doi.org/10.14233/ajchem.2025.33973
Sangeeta Gupta
Department of Chemistry, School of Sciences, IFTM University, Moradabad-244102, India
https://orcid.org/0009-0004-7278-1704
Amanpreet Kaur
Department of Chemistry, School of Sciences, IFTM University, Moradabad-244102, India
https://orcid.org/0000-0002-3162-2844
Neha Bhatt
Department of Chemistry, Pt. L.M.S., Rishikesh Campus, Shri Dev Suman Uttarakhand Vishwavidyalay, Rishikesh-249201, India
https://orcid.org/0000-0002-6333-5145
Man Vir Singh
School of Applied and Life Sciences, Uttaranchal Institute of Technology, Uttaranchal University, Dehradun-248007, India
https://orcid.org/0000-0002-7498-6066
Mansi Rani
Department of Chemistry, School of Sciences, IFTM University, Moradabad-244102, India
https://orcid.org/0009-0007-0259-2010
Shubhangee Agarwal
Department of Chemistry, School of Sciences, IFTM University, Moradabad-244102, India
https://orcid.org/0000-0002-1173-358X

Corresponding Author(s) : Amanpreet Kaur

amanpreet2225@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 11 (2025): Vol 37 Issue 11, 2025

Abstract

In this study, the biosynthesis of silver nanoparticles (AgNPs) was achieved using Raphanus sativus (radish) aqueous extract acted as both a reducing and stabilizing agent. The formation of AgNPs was confirmed through visual colour change and characterized by various spectroscopic and microscopic techniques. UV-Vis spectroscopy confirmed the successful synthesis of AgNPs with a distinct absorption peak at 432.054 nm. Fourier-transform infrared (FT-IR) spectroscopy revealed absorption bands indicative of phytoconstituents acting as capping agents. Field emission scanning electron microscopy (FESEM) provided morphological insights, while X-ray diffraction (XRD) analysis deter-mined an average crystalline size of 20.35 nm. The photocatalytic efficiency of biosynthesized AgNPs was evaluated by the breakdown of methylene blue dye molecules during UV light exposure. The DPPH and ABTS radical scavenging assays were used to assess antioxidant activity, yielding IC50 values of 22.43 µg/mL and 30.54 µg/mL, respectively. The significant inhibitory effects were observed while assessing antibacterial efficacy against Gram-positive (Listeria monocytogenes and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. A significant, dose-dependent inhibitory effect was observed against all tested bacterial strains, as indicated by the zones of inhibition. Furthermore, the inhibition experiments for α-amylase and α-glucosidase demonstrated encouraging antidiabetic effects, yielding IC50 values of 210 µg/mL and 185 µg/mL, respectively. Furthermore, the photocatalytic activity of the biosynthesized AgNPs was assessed using the degradation of methylene blue under light irradiation, confirming their potential application in environmental remediation. Overall, the study highlights the multifunctional properties of Raphanus sativus-mediated silver nanoparticles and their promising applications in biomedical and environmental fields.

Keywords

Green synthesis Raphanus sativus AgNPs Photocatalytic degradation Biological activities
(1)
Gupta, S.; Kaur, A.; Bhatt, N.; Singh, M. V.; Rani, M.; Agarwal, S. Green Synthesis of Silver Nanoparticles Using Raphanus Sativus Water Extract for Enhanced Antimicrobial and Photocatalytic Activities. ajc 2025, 37, 2773-2780.
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