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Vol. 26 No. 22 (2014): Vol 26 Issue 22

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Aptamer-Based Fluorescence Polarimetric Assay for the Sensitive Detection of Immunoglobulin E in Human Serum

https://doi.org/10.14233/ajchem.2014.14081
Xiaohuan Qin
Key Laboratory for the Chemistry and Molecular Engineering of Medi cinal Resources (Ministry of Education of China), School of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin 541004, P.R. China

Corresponding Author(s) : Xiaohuan Qin

qinchengshi091129@163.com

Asian Journal of Chemistry, Vol. 26 No. 22 (2014): Vol 26 Issue 22

Abstract

A highly sensitive and selective fluorescence polarization method was developed for the determination of immunoglobulin E based on the aptamer specificity binding immunoglobulin E event into a detectable fluorescence polarization signal. Using this method, the levels of immunoglobulin E in human serum from healthy donors were determined. The results show that the fluorescence polarization method has excellent specificity against target protein and high sensitivity in complex medium and is applicable to the no-separation based detection of proteins in biological sample.

Keywords

Immunoglobulin E Fluorescence polarization Aptamer
Qin, X. (2014). Aptamer-Based Fluorescence Polarimetric Assay for the Sensitive Detection of Immunoglobulin E in Human Serum. Asian Journal of Chemistry, 26(22), 7491–7493. https://doi.org/10.14233/ajchem.2014.14081
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