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Vol. 36 No. 10 (2024): Vol 36 Issue 10, 2024

Copyright (c) 2024 Dr G SURESH SURESH, K. Dhanaraj, R.N. Viswanath, B. Dhanalakshmi, A. Sindhya

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Antibacterial and Cell-Viability of Hydroxyapatite Derived from Waste Marine Shell for Biomedical Applications

https://doi.org/10.14233/ajchem.2024.32287
G. Suresh
Department of Physics, Centre for Nanotechnology Research, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation (D U), Chennai-603104, India
https://orcid.org/0000-0002-2525-1231
K. Dhanaraj
Department of Physics, St. Joseph’s College of Engineering, Sholinganallur, Chennai, India
https://orcid.org/0000-0001-5504-3604
R.N. Viswanath
Department of Physics, Centre for Nanotechnology Research, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation (D U), Chennai-603104, India
https://orcid.org/0000-0002-6772-7672
B. Dhanalakshmi
Department of Physics, Centre for Nanotechnology Research, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation (D U), Chennai-603104, India
https://orcid.org/0000-0002-9001-3395
A. Sindhya
Department of Science and Humanities/Physics, I.F.E.T. College of Engineering (Autonomous), Villupuram-605108, India
https://orcid.org/0000-0002-1520-3527

Corresponding Author(s) : G. Suresh

suresh.physics@avit.ac.in

Asian Journal of Chemistry, Vol. 36 No. 10 (2024): Vol 36 Issue 10, 2024

Abstract

The present study discusses on the functional groups, structural characterization and thermal behaviour of Crassostrea angulata shell used for the synthesis of a biocompatible hydroxyapatite material. The hydroxyapatite was synthesized from C. angulata shell through precipitation method and characterized using XRD, FTIR, FE-SEM, EDX mapping, HR-TEM and SAED pattern. The formation of hydroxyapatite was confirmed from the high intense X-ray diffraction lines and by the presence of chemical groups such as PO43–, OH– and CO32–. The structural changes during the formation of hydroxyapatite from the thermal decomposition of C. angulata shell were studied through XRD and TG-DTA. The morphology study by FE-SEM indicates that the synthesized hydroxyapatite materials were in the form of clusters of rod-like particles. The SAED patterns and XRD spectral lines confirmed the crystalline nature of the synthesized hydroxyapatite. The antibacterial activity and cell viability assay of prepared hydroxyapatite were studied to assess its suitability in various biomedical applications. The antibacterial activity of hydroxyapatite was assessed against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus saprophyticus) bacterial strains. The cytocompatibility of hydroxyapatite was assessed through an MTT assay using osteoblast cells (MG-63 cell). Thus, the prepared hydroxyapatite shows good antibacterial action and cell viability nature and it can be applied in various biomedical applications.

Keywords

Crassostrea angulata Hydroxyapatite Antibacterial activity Cell viability
Suresh, G., Dhanaraj, K., Viswanath, R., Dhanalakshmi, B., & Sindhya, A. (2024). Antibacterial and Cell-Viability of Hydroxyapatite Derived from Waste Marine Shell for Biomedical Applications. Asian Journal of Chemistry, 36(10), 2293–2299. https://doi.org/10.14233/ajchem.2024.32287
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References
  1. S. Mondal, S.n Park, J. Choi, T.T.H. Vu, V.H.M.h Doan, T.T. Vo, B. Lee and J. Oh, Adv. Colloid Interface Sci., 321 103013 (2023); https://doi.org/10.1016/j.cis.2023.103013
  2. S.-Y. Peng, Y.-W. Lin, W.-H. Lee, Y.-Y. Lin, M.-J. Hung and K.-L. Lin, Mater. Chem. Phys., 293 126854 (2023); https://doi.org/10.1016/j.matchemphys.2022.126854
  3. R. Verma, S.R. Mishra, V. Gadore and M. Ahmaruzzaman, Adv. Colloid Interface Sci., 315 102890 (2023); https://doi.org/10.1016/j.cis.2023.102890
  4. M.B. Mobaraka, N.S. Pinky, F. Chowdhury, M.S. Hossain, M. Mahmud, M.S. Quddus, S.A. Jahan and S. Ahmed, J. Saudi Chem. Soc., 27 101690 (2023); https://doi.org/10.1016/j.jscs.2023.101690
  5. M. Wang, M. Li, Y. Wang, Y. Shao, Y.n Zhu and S.g Yang, J. Mater. Chem. B, 9, 3401 (2021); https://doi.org/10.1039/D1TB00098E
  6. S. Lamkhao, M. Phaya, C. Jansakun, K. Thongkorn, G. Rujijanagul, N. Chandet, P. Bangrak and C. Randorn, Sci. Rep., 9 4015 (2019); https://doi.org/10.1038/s41598-019-40488-8
  7. K. Sinulingga, M. Sirait, N. Siregar and M.E. Doloksaribu, ACS Omega, 6, 34185 (2021); https://doi.org/10.1021/acsomega.1c05921
  8. W. Li, J. Zhou and Y. Xu, Biomed. Rep., 3, 617 (2015); https://doi.org/10.3892%2Fbr.2015.481
  9. S. Lamkhao, M. Phaya, C. Jansakun, N. Chandet, K. Thongkorn, G. Rujijanagul, P. Bangrak and C. Randorn, Sci. Rep., 9, 4015 (2019); https://doi.org/10.1038/s41598-019-40488-8
  10. S. Sebastiammal, A.S.L. Fathima, J. Henry, M.A. Wadaan, S. Mahboob, A.M. Wadaan, I.n Manzoor, I. Manzoor, K. Gopinath, M. Rajeswary and M. Govindarajan, Fermentation, 8, 677 (2022); https://doi.org/10.3390/fermentation8120677
  11. J. Kolmas, E. Groszyk and D. Kwiatkowska-Rohycka, BioMed Res. Int., 2014, 178123, (2014); https://doi.org/10.1155/2014/178123
  12. K. Pajor, A. Michalicha, A. Belcarz, L. Pajchel, A. Zgadzaj, F. Wojas and J. Kolmas, Int. J. Mol. Sci., 23, 7102 (2022); https://doi.org/10.3390/ijms23137102
  13. C.B. Ayyanar, K. Marimuthu, B. Gayathri and Sankarrajan, Polym. Polym. Composit., 29, 1534 (2021); https://doi.org/10.1177/0967391120981551
  14. M. Sirait, K. Sinulingga, N. Siregar, M.E. Doloksaribu and Amelia, J. Phys.: Conf. Series, 2193, 012039 (2022); https://doi.org/10.1088/1742-6596/2193/1/012039
  15. G.T. El-Bassyouni, S.S. Eldera, S.H. Kenawy and E.M.A. Hamzawy, Heliyon, 6, e04085 (2020); https://doi.org/10.1016/j.heliyon.2020.e04085
  16. J. Han, X.-Y. Tong, C.-Y. Rao, J.-M. Ouyang and B.-S. Gui, ACS Omega, 8, 48432 (2023); https://doi.org/10.1021/acsomega.3c08180
  17. H.Y. Ahmed, N. Safwat, R. Shehata, E.H. Althubaiti, S. Kareem, A. Atef, S.H. Qari, A.H. Aljahani, A.S. Al-Meshal, M. Youssef and R. Sami, Membranes, 12, 408 (2022); https://doi.org/10.3390/membranes12040408
  18. K. Manivannan, G. Jaganathan and M.A. Sithique, Asian J. Chem., 34, 1073 (2022); https://doi.org/10.14233/ajchem.2022.23270
  19. M. Cheng, M. Liu, L. Chang, Q.g Liu, C.o Wang, L. Hu, Z. Zhang, W. Ding, L. Chen, S. Guo, Z. Qi, P. Pan and J. Chen, Sci. Total Environ., 870, 161950 (2023); https://doi.org/10.1016/j.scitotenv.2023.161950
  20. C.S. Kumar, K. Dhanaraj, R.M. Vimalathithan, P. Ilaiyaraja and G. Suresh, J. Asian Ceramic Soc., 8, 416 (2020); https://doi.org/10.1080/21870764.2020.1749373
  21. G. Borciani, T. Fischetti, G.a Ciapetti, M. Montesissa, N. Baldini and G. Graziani, Ceram. Int., 49, 1572 (2023); https://doi.org/10.1016/j.ceramint.2022.10.341
  22. N. Suwannasingha, A.t Kantavong, S. Tunkijjanukij, C. Aenglong, H.-B. Liu and W. Klaypradit, J. Saudi Chem. Soc., 26, 101441 (2022); https://doi.org/10.1016/j.jscs.2022.101441
  23. M. Rehman, M.B. Taj and S.A.C. Carabineiro, Chemosphere, 338, 139477 (2023); https://doi.org/10.1016/j.chemosphere.2023.139477
  24. C.-F. Chen, Y.-R. Ju, Y.C. Lim, M.-H. Wang, C.-W. Chen and C.-D. Dong, Marine Pollut. Bull., 194 115228 (2023); https://doi.org/10.1016/j.marpolbul.2023.115228
  25. K. Dhanaraj and G. Suresh, Vacuum, 152, 222 (2018); https://doi.org/10.1016/j.vacuum.2018.03.021
  26. Z. Zhou, Y. Wang, S. Sun, Y.g Wang and L. Xu, Heliyon, 8, e11938 (2022); https://doi.org/10.1016/j.heliyon.2022.e11938
  27. Y.C. Wong and R.X. Ang, Open Chem., 16, 1166 (2018); https://doi.org/10.1515/chem-2018-0127
  28. K. Dhanaraj, C.S. Kumar and G. Suresh, J. Appl. Sci. Comput., 5, 658 (2018).
  29. R.A. Youness, M.A. Taha, H Elhaes and M. Ibrahim, Mater. Chem. Phys., 190, 209 (2017); https://doi.org/10.1016/j.matchemphys.2017.01.004
  30. I.R. Mary, S. Sonia, D. Mangalaraj, C. Viswanathan, N. Ponpandian and S. Viji, Appl. Surf. Sci., 361, 25 (2016); https://doi.org/10.1016/j.apsusc.2015.11.123
  31. M. Sadat-Shojai, M. Atai and A. Nodehi, J. Braz. Chem. Soc., 22, 571 (2011); https://doi.org/10.1590/S0103-50532011000300023
  32. R.K. Saini, L.P. Bagri and A.K. Bajpai, Colloids Surf. B Biointerfaces, 177, 211 (2019); https://doi.org/10.1016/j.colsurfb.2019.01.064
  33. M. Manoj, R. Subbiah, D. Mangalaraj, N. Ponpandian, C. Viswanathan and K. Park, Nanobiomedicine, 2, 1 (2015); https://doi.org/10.5772/60116
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