Biofunctional Potential of Luteolin-Loaded Sodium Alginate–Chitosan Nanoparticles: Antioxidant, Antihyperglycemic and Antiglycation Activities

M.S. Priyadharshini; Gayathri Devi Sundaramurthy; Kandavelmani Angamuthu

doi:10.14233/ajchem.2026.36974

Issue

Vol. 38 No. 10 (2026): Vol 38, Issue 10, 2026

Issue Published : 9 October 2026

This work is licensed under a Creative Commons Attribution 4.0 International License.

Biofunctional Potential of Luteolin-Loaded Sodium Alginate–Chitosan Nanoparticles: Antioxidant, Antihyperglycemic and Antiglycation Activities

https://doi.org/10.14233/ajchem.2026.36974

M.S. Priyadharshini

Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641043, India

https://orcid.org/0009-0002-9755-2030

Gayathri Devi Sundaramurthy

Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641043, India

https://orcid.org/0000-0002-3908-7671

Kandavelmani Angamuthu

Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641043, India

https://orcid.org/0000-0003-0402-6307

Corresponding Author(s) : Gayathri Devi Sundaramurthy

gayathridevi_bc@avinuty.ac.in

Asian Journal of Chemistry, Vol. 38 No. 10 (2026): Vol 38, Issue 10, 2026
Article Published : 9 October 2026

Abstract

The present study reports the in vitro antioxidant, enzyme inhibitory, glucose diffusion, yeast-model glucose uptake-related and antiglycation activities of luteolin-loaded sodium alginate–chitosan nanoparticles (LLCNPs). LLCNPs exhibited concentration-dependent DPPH radical-scavenging activity and ferric-reducing antioxidant power (FRAP). In the DPPH assay, LLCNPs showed an IC₅₀ value of 5.95 µg/mL, compared with 6.86 µg/mL for ascorbic acid. The FRAP assay showed a concentration-dependent increase in the ferric reducing activity of LLCNPs, with the highest response observed at 100 µg/mL. LLCNPs also demonstrated concentration-dependent α-amylase and α-glucosidase inhibitory activities. In the glucose diffusion assay, LLCNPs reduced glucose diffusion across the dialysis membrane under the experimental conditions. The yeast-model assay demonstrated glucose uptake-related activity as indicated by a reduction in residual glucose in the presence of LLCNPs. LLCNPs also exhibited antiglycation activity across the tested concentration range. Comparative evaluation with luteolin, blank nanoparticles and reference compounds demonstrated differences among the tested formulations. These findings confirm that LLCNPs exhibit several measurable in vitro biofunctional activities.

Keywords

Luteolin Sodium alginate-chitosan Antidiabetic activity Antioxidant activity Anti-glycation

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Priyadharshini, M.; Sundaramurthy, G. D.; Angamuthu, K. Biofunctional Potential of Luteolin-Loaded Sodium Alginate–Chitosan Nanoparticles: Antioxidant, Antihyperglycemic and Antiglycation Activities. ajc 2026, 38, 2743-2750.

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B. Manjula and G. Jothi, J. Stress Physiol. Biochem., 19, 47 (2023).

M. Thakur, R. Verma, D. Kumar, S. Manickam, R. Ullah, M. A. Ibrahim, A. Bari, H. Lalhenmawia, and D. Kumar, Heliyon, 10, e39165 (2024); https://doi.org/10.1016/j.heliyon.2024.e39165

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H. El Allaoui, K. Haboubi, K. El Ahmadi, M. Bouhrim, A. ElAbdouni, B. Eto, A.A. Shahat, R.N. Herqash, M. El Bestrioui, Z. Zouaoui and M. Nhiri, Front. Pharmacol., 16, 1551841 (2025); https://doi.org/10.3389/fphar.2025.1551841

Author biographies is not available.