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Vol. 27 No. 10 (2015): Vol 27 Issue 10

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Isolation and Structural Elucidation of Bioactive Compound from Ethanolic Extract of Cassia tora Leaves

https://doi.org/10.14233/ajchem.2015.18952
Rajesh Bakoriya
Department of Botany, Shaheed Bhagat Singh Government Degree College, Ashta-466 116, India
Kapil K. Soni
Department of Bioscience, Barkatullah University, Bhopal-462 026, India
Tessy Thomas
Department of Botany, Chandra Shekhar Azad Government Post Graduate Nodal College, Sehore-466 001, India

Corresponding Author(s) : Kapil K. Soni

kapilsoni14@gmail.com

Asian Journal of Chemistry, Vol. 27 No. 10 (2015): Vol 27 Issue 10

Abstract

Present study describes the isolation of extract from the leaves of plant Cassia tora (Linn.). During the present study, fresh leaves after shade drying was used for the isolation of extract using Soxhlet apparatus and percentage yield was obtained (3.22 % in ethanol). Then, preliminary phyto-chemical screening of the extract was done and certain secondary metabolites viz. alkaloids, glycosides, saponin, triterpenes, tannin and flavonoid were confirmed in the Cassia tora extract. Then thin layer and column chromatography of the extract was done and Rf value viz. 0.91, 0.96 and 1.00 % were calculated and obtained fractions (CT1 to CT5) were tested on Wistar albino rats for wound healing activities. Active fraction of extract were sent to SAIF, CDRI Lucknow for spectral analysis viz. IR, UV, 1H NMR, 13C NMR and Mass for the detection/confirmation of bio-active compound present in the fraction and finally emodin was elucidated.

Keywords

Bioactive compounds Cassia tora Secondary metabolites Traditional medicines
Bakoriya, R., K. Soni, K., & Thomas, T. (2015). Isolation and Structural Elucidation of Bioactive Compound from Ethanolic Extract of Cassia tora Leaves. Asian Journal of Chemistry, 27(10), 3749–3752. https://doi.org/10.14233/ajchem.2015.18952
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