Issue

Vol. 35 No. 12 (2023): Vol 35 Issue 12, 2023

Copyright (c) 2023 J. David Blessing Rani, C. Asha Deepti

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Liquid Chromatographic-Electron Spray Ionization-Mass Spectroscopy Technique for the Simultaneous Determination of Ethinyl Estradiol & Etonogestrel and Characterization of New Forced Degradation Compounds

https://doi.org/10.14233/ajchem.2023.30700
J. David Blessing Rani
Department of Pharmaceutical Analysis, GITAM School of Pharmacy, GITAM (Deemed to be University), Visakhapatnam-530045, India School of Pharmacy, Centurion University of Technology and Management, Balasore-756044, India
https://orcid.org/0009-0006-6592-1814
C. Asha Deepti
Department of Pharmaceutical Analysis, GITAM School of Pharmacy, GITAM (Deemed to be University), Visakhapatnam-530045, India
https://orcid.org/0000-0003-2276-9497

Corresponding Author(s) : J. David Blessing Rani

ranijakkala@gmail.com

Asian Journal of Chemistry, Vol. 35 No. 12 (2023): Vol 35 Issue 12, 2023

Abstract

The aim of the current study was to develop, validate a precise, rapid, accurate liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous estimation of ethinyl estradiol and etonogestrel. The retention times of ethinyl estradiol and etonogestrel were found at 3.112 min and 4.399 min, respectively, whereas the linear range were found in the concentration ranges from 0.04-0.23 µg/mL & 3-18 µg/mL with slope coefficient (R2) of 0.9992 and 0.9991 correspondingly. Similarly, the accuracy values for ethinyl estradiol and etonogestrel were found to be 100.5% and 100.1% correspondingly. As per ICH Q2(R1) guidelines, the method was validated with liquid chromatography-mass spectrometry to confirm the newly obtained degradation compounds chemical structures of ethinyl estradiol and etonogestrel.

Keywords

Ethinyl estradiol Etonogestrel LC-MS Accuracy
Rani, J. D. B., & Deepti, C. A. (2023). Liquid Chromatographic-Electron Spray Ionization-Mass Spectroscopy Technique for the Simultaneous Determination of Ethinyl Estradiol & Etonogestrel and Characterization of New Forced Degradation Compounds. Asian Journal of Chemistry, 35(12), 3105–3110. https://doi.org/10.14233/ajchem.2023.30700
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Y.V. Karpievitch, A.D. Polpitiya, G.A. Anderson, R.D. Smith and A.R. Dabney, Ann. Appl. Stat., 4, 1797 (2010); https://doi.org/10.1214/10-AOAS341
  2. A. Chandewar, Pharm. Anal. Acta, 4, 262 (2013); https://doi.org/10.4172/2153-2435.1000262
  3. A. Gandhi, S. Guttikar and P. Trivedi, J. Pharm. Anal., 5, 316 (2015); https://doi.org/10.1016/j.jpha.2015.02.002
  4. R.S. Berkowitz, R.L. Barbieri, A.E. Dunaif, K.J. Ryan and R.L. Barbieri, Kistner’s Gynecology and Women’s Health, St. Louis, Missouri, pp 345-393 (1999).
  5. K.V. Moray, H. Chaurasia, O. Sachin and B. Joshi, Reprod. Health, 18, 4 (2021); https://doi.org/10.1186/s12978-020-01054-y
  6. J. Grentzer, C. McNicholas and J.F. Peipert, Expert Rev. Obstetr. Gynecol., 8, 337 (2013); https://doi.org/10.1586/17474108.2013.811941
  7. G. Petro, T. Spence, M. Patel, A.M. Gertz and C. Morroni, Eur. J. Obstet. Gynecol. Reprod. Biol., 224, 81 (2018); https://doi.org/10.1016/j.contraception.2020.04.013
  8. L. Thaxton and A. Lavelanet, Int. J. Gynecol. Obstertr., 144, 2 (2019); https://doi.org/10.1002/ijgo.12696
  9. G.V. Hines, J.N. Quinones, T.N. Walker and A. Waxman, Obstertr. Gynecol., 139, 10S (2022); https://doi.org/10.1097/01.AOG.0000826464.75316.d6
  10. T.L. Lemke and D.A. Williams, Foye’s Principles of Medicinal Chemistry, Lippincott Williams & Wilkins, p. 1409 (2012).
  11. H. Hognert, H.K. Kallner, S. Cameron, C. Nyrelli, I. Jawad, R. Heller, A. Aronsson, I. Lindh, L. Benson and K. Gemzell-Danielsson, Human Reprod., 31, 2484 (2016); https://doi.org/10.1093/humrep/dew238
  12. M.I. Toral, F. Nacaratte and F. Nova, Anal. Lett., 6, 1009 (2014); https://doi.org/10.1080/00032719.2014.968930
  13. J.J. Berzas, J. Rodríguez and G. Castañeda, Analyst, 122, 41 (1997); https://doi.org/10.1039/a604558h
  14. S.G. Nair, D.P. Patel, J.G. Frank, B.M. Patel, P. Singhal and D.V. Chaudhary, Biomed. chromatogr., 32, e4165S (2018); https://doi.org/10.1002/bmc.4165
  15. H. Licea-Perez, S. Wang, C.L. Bowen and E. Yang, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 852, 69 (2007); https://doi.org/10.1016/j.jchromb.2006.12.052
  16. M.J. López de Alda and D. Barceló, J. Chromatogr. A, 892, 391 (2000); https://doi.org/10.1016/S0021-9673(00)00068-6
  17. A. Singh, B. Mishra, N. Sharma and K. Mishra, Int. J. Chem. Pharm. Anal., 1, 80 (2014).
  18. M Sarat, A Rambabu, Int. J. Pharm. Pharm. Sci., 5, 115 (2012).
  19. K. Shaikh, A. Patil and S. Katare, J. Trace Anal. Food Drugs, 1, 30 (2013); https://doi.org/10.7726/jtafd.2013.1004
Read More
Author biographies is not available.
Statistic
Read Counter : 0 Download : 0