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Vol. 38 No. 7 (2026): Vol. 38, No 7 (2026)

Copyright (c) 2026 Ashok Singh

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Synthesis, Characterisation, Biological Activity and Molecular Docking of Metal(II) Complexes with Schiff Base Derived from o-Vanillin and 4-Chloro-o-phenylenediamine

https://doi.org/10.14233/ajchem.2026.35910
Ashok Singh
Department of Chemistry, V.S.S.D. College, Kanpur-208002, India
https://orcid.org/0009-0004-4282-6182
Manju Yadav
Department of Chemistry, Maharana Pratap Government P.G. College, Hardoi-241001, India
https://orcid.org/0009-0009-5712-8316
Kuldeep Kumar
Department of Chemistry, Maharana Pratap Government P.G. College, Hardoi-241001, India
https://orcid.org/0009-0007-9138-3957
Shivam Maurya
Department of Chemistry, Maharana Pratap Government P.G. College, Hardoi-241001, India
https://orcid.org/0000-0002-2226-3683
Saurabh Kumar Singh
Department of Chemistry, Lucknow University, Lucknow-226007, India
https://orcid.org/0009-0000-3044-5236
Jaya Dwivedi
Department of Chemistry, Banasthali Vidyapeeth, Banasthali-304022, India
https://orcid.org/0000-0002-1524-7714
Nand Lal
Department of Chemistry, V.S.S.D. College, Kanpur-208002, India
https://orcid.org/0009-0009-3165-7244

Corresponding Author(s) : Nand Lal

drnandlal71@gmail.com

Asian Journal of Chemistry, Vol. 38 No. 7 (2026): Vol. 38, No 7 (2026)

Abstract

This work reports the synthesis, structural elucidation and characterization of the tetradentate Schiff base H2L (bis[3-methoxy-salicylidene]-4-chloro-o-phenylenediamine) and its Cu(II) and Ni(II) coordination compounds, respresented as CuL and NiL, respectively. The Schiff base H2L was synthesized via condensation of o-vanillin and 4-chloro-o-phenylenediamine in a 2:1 molar ratio. The ligand and its Cu(II) and Ni(II) complexes were characterized using elemental analysis, ESI-MS, HRMS, FTIR, UV-visible, 1H NMR, 13C NMR spectroscopy and thermogravimetric analysis (TGA). Molar conductance measurements confirmed the non-electrolytic nature of both complexes in solution. Based on the spectroscopic and analytical data, the CuL and NiL complexes were assigned distorted square-planar geometries. Powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) were employed to investigate their crystallinity and surface morphology. The antimicrobial potential of H2L, CuL and NiL was evaluated in vitro using the agar well diffusion method and in silico through molecular docking studies against target proteins from Escherichia coli (PDB ID: 1KZN), Pseudomonas aeruginosa (PDB ID: 4CL6), Staphylococcus aureus (PDB ID: 3FYV) and Candida albicans (PDB ID: 5FSA). The results were further correlated with the experimentally determined antimicrobial activities.

Keywords

Schiff base Metal(II) complex Spectral studies Biocidal efficacy Molecular docking
(1)
Singh, A.; Yadav, M.; Kumar, K.; Maurya, S.; Singh, S. K.; Dwivedi, J.; Lal, N. Synthesis, Characterisation, Biological Activity and Molecular Docking of Metal(II) Complexes With Schiff Base Derived from O-Vanillin and 4-Chloro-O-Phenylenediamine. ajc 2026, 38, 1696-1706.
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