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Vol. 38 No. 5 (2026): Vol 38, Issue 5, 2026

Copyright (c) 2026 Prashanth M K

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Anticancer Potential of Isolated Gymnemic Acid-III from Gymnema sylvestre: Insights from In vitro and In silico Analysis

https://doi.org/10.14233/ajchem.2026.35623
Swetha Madamala
Department of Chemistry, B.N.M. Institute of Technology, Bengaluru-560070, India
https://orcid.org/0000-0002-3297-7647
B.S. Prathibha
Department of Chemistry, B.N.M. Institute of Technology, Bengaluru-560070, India
https://orcid.org/0000-0002-6902-3169
G.S. Poornima
Department of Chemistry, New Horizon College of Engineering, Bangalore-560103, India
https://orcid.org/0000-0001-5824-2078
S. Balasaraswathy
Department of Physics, B.N.M. Institute of Technology, Bengaluru-560070, India
https://orcid.org/0000-0002-4166-743X
M.K. Prashanth
Department of Chemistry, B.N.M. Institute of Technology, Bengaluru-560070, India
https://orcid.org/0000-0001-8526-9241

Corresponding Author(s) : M.K. Prashanth

prashanthmk87@gmail.com

Asian Journal of Chemistry, Vol. 38 No. 5 (2026): Vol 38, Issue 5, 2026

Abstract

Gymnemic acid-III is a naturally occurring triterpenoid saponin isolated from the leaves of Gymnema sylvestre, a medicinal plant widely recognized for its therapeutic potential. The current study attempts to isolate, characterize and assess its anticancer properties. Gymnemic acid-III was obtained by purifying the crude saponins from aqueous extraction using preparative HPLC. Using FT-IR, 13C NMR and LC-MS investigations, the structural elucidation was accomplished. In comparison to the reference drug ursolic acid, cytotoxicity tests of gymnemic acid-III were employing the MCF-7 and A549 cell lines demonstrated concentration-dependent inhibitory effects. Molecular docking studies against the α- and β-tubulin (PDB ID: 1JFF) target protein demonstrated a significant binding affinity with a docking score of -10.08 kcal/mol. Gymnemic acid-III was classified as non-acutely toxic profile from computational toxicity prediction. The results highlight gymnemic acid-III is a promising natural molecule for further investigation as a lead compound for anticancer treatment.

Keywords

Gymnema sylvestre Gymnemic acid Cytotoxicity Molecular docking Apoptosis
(1)
Madamala, S.; Prathibha, B.; Poornima, G.; Balasaraswathy, S.; Prashanth, M. Anticancer Potential of Isolated Gymnemic Acid-III from Gymnema Sylvestre: Insights from In Vitro and In Silico Analysis. ajc 2026, 38, 1189-1195.
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