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Vol. 27 No. 2 (2015): Vol 27 Issue 2

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Metal Ion Complexes of Kaempferitrin as DNA Topoisomerase I Inhibitors and Its' Cytotoxicities

https://doi.org/10.14233/ajchem.2015.16997
Xi-Lin Ouyang
Youjiang Medical University for Nationalities, Baise 533000, P.R. China
Zhi-Ren Zhu
State Key Laboratory Cultivation Base for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China
Ying-Ming Pan
State Key Laboratory Cultivation Base for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China
Heng-Shan Wang
State Key Laboratory Cultivation Base for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China

Corresponding Author(s) : Ying-Ming Pan

panym2005@sina.com

Asian Journal of Chemistry, Vol. 27 No. 2 (2015): Vol 27 Issue 2

Abstract

In this study, four binuclear M(II) (M = Cu, Zn, Co and Cd) complexes of kaempferitrin were synthesized. Their structures were characterized by elemental analysis, infrared spectroscopy, TG-DTG analysis and MS spectra. The activities of kaempferitrin and its metal complexes on DNA topoisomerase I (TOPO I) were measured by agarose gel electrophoresis using plasmids pBR322 and pUC19 as model substrate DNA. The cytotoxicities of these compounds on MCF-7, SGC-7901, SK-OV-3 and HePG-2 cell lines were determined by MTT assay. The results showed that four complexes could significantly affect the performance of DNA TOPO I to promote its mediated DNA helicase or breakage. Copper complex was identified as an excellent TOPO I Inhibitor. Furthermore, copper complex can inhibit the proliferation of SGC-7901 and SK-OV-3 with the inhibition rate of 44.19 and 44.58 %, stronger than that of the kaempferitrin and other complexes. This study has proved that the kaempferitrin complexes have stronger inhibitory effects than kaempferitrin for potential synergistic effects.

Keywords

Kaempferitrin Complex Topoisomerase I Inhibitor Cytotoxicity
Ouyang, X.-L., Zhu, Z.-R., Pan, Y.-M., & Wang, H.-S. (2015). Metal Ion Complexes of Kaempferitrin as DNA Topoisomerase I Inhibitors and Its’ Cytotoxicities. Asian Journal of Chemistry, 27(2), 503–506. https://doi.org/10.14233/ajchem.2015.16997
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