Copyright (c) 2026 Pooja Vartiya, Piyush Kashyap

This work is licensed under a Creative Commons Attribution 4.0 International License.
Impact of Phenolic Removal on the Molecular and Functional Properties of Sunflower Seed Cake Proteins
Corresponding Author(s) : Piyush Kashyap
Asian Journal of Chemistry,
Vol. 38 No. 4 (2026): Vol 38 Issue 4, 2026
Abstract
Sunflower seed cake (SSC), the major by-product of oil extraction, is considered an under utilised protein resource due to its abundance. The nutritional quality and functionality of protein fractions in SSC are negatively affected by phenolic compounds. This study compared the functional and nutritional properties of phenolised and dephenolised protein fractions. Dephenolisation using ethanol extraction reduced the total phenolic content significantly and improved the purity and yield of proteins. Significant improvements were recorded in functional traits: water-holding capacity, oil-holding capacity, emulsifying activity and foaming capacity, after the removal of phenolics. Nutritionally, dephenolised fractions had higher purities and yields due to exposure of active sites that were being held internally within phenolic complexes. Of all fractions, globulin and albumin elicited better functional and nutritional performance upon dephenolisation. Overall, these findings confirm dephenolisation as an appropriate pretreatment to enhance protein functionality in SSC, supporting its application in sustainable food formulation and protein-enriched products.
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- P. Kashyap, C. Singh Riar and N. Jindal, Biomass Convers. Biorefin., 14, 4883 (2024); https://doi.org/10.1007/s13399-022-02740-6
- X. Huang, Y. Li, C. Cui and D. Sun-Waterhouse, Food Chem., 439, 138090 (2024); https://doi.org/10.1016/j.foodchem.2023.138090
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- I.D. Nwachukwu and R.E. Aluko, Food Chem., 255, 216 (2018); https://doi.org/10.1016/j.foodchem.2018.02.068
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References
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F. Girotto, M. Merlino, G. Giovanelli, C. Condurso and L. Piazza, Int. J. Food Sci. Technol., 59, 4784 (2024); https://doi.org/10.1111/ijfs.17208
M. Cisneros-Yupanqui, V.I. Chalova, H.R. Kalaydzhiev, D. Mihaylova, A.I. Krastanov and A. Lante, Food Bioprocess Technol., 14, 1962 (2021); https://doi.org/10.1007/s11947-021-02695-y
B.G. Subaşı, F. Casanova, E. Capanoglu, F. Ajalloueian, J.J. Sloth and M.A. Mohammadifar, Food Biosci., 38, 100749 (2020); https://doi.org/10.1016/j.fbio.2020.100749
P. Vartiya, P. Kashyap, B. Raj, M. Sharma, M. Adnan, S.A. Ashraf and R. Mehra, J. Agric. Food Res., 22, 102088 (2025); https://doi.org/10.1016/j.jafr.2025.102088
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AOAC, Official Methods of Analysis of AOAC International, Rockville, MD, USA: AOAC International, edn. 20 (2016).
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S. Deb, Y. Kumar and D.C. Saxena, Food Chem. X, 13, 100205 (2022); https://doi.org/10.1016/j.fochx.2022.100205
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R. Kaur and G. Ghoshal, Adv. Colloid Interface Sci., 306, 102725 (2022); https://doi.org/10.1016/j.cis.2022.102725
M.A. Malik, H.K. Sharma and C.S. Saini, J. Food Sci. Technol., 53, 3455 (2016); https://doi.org/10.1007/s13197-016-2320-y
U.C. Jha, H. Nayyar, S.K. Parida, R. Deshmukh, E.J. von Wettberg and K.H. Siddique, Int. J. Mol. Sci., 23, 7710 (2022); https://doi.org/10.3390/ijms23147710
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M. Borchani, H. Yaich, F. Abbès, C. Blecker, S. Besbes, H. Attia and M. Masmoudi, Waste Biomass Valoriz., 12, 1749 (2021); https://doi.org/10.1007/s12649-020-01111-4
Y. Feng, C. Jin, S. Lv, H. Zhang, F. Ren and J. Wang, Antioxidants, 12, 1577 (2023); https://doi.org/10.3390/antiox12081577
R. Kaur, G. Ghoshal and S. Chauhan, J. Food Meas. Charact., 18, 3708 (2024); https://doi.org/10.1007/s11694-024-02443-8
P. Kashyap, C. Singh Riar and N. Jindal, Biomass Convers. Biorefin., 14, 4883 (2024); https://doi.org/10.1007/s13399-022-02740-6
X. Huang, Y. Li, C. Cui and D. Sun-Waterhouse, Food Chem., 439, 138090 (2024); https://doi.org/10.1016/j.foodchem.2023.138090
B. Masoumi, M. Tabibiazar, Z. Golchinfar, M. Mohammadifar and H. Hamishehkar, Crit. Rev. Food Sci. Nutr., 64, 3539 (2024); https://doi.org/10.1080/10408398.2022.2132376
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I.D. Nwachukwu and R.E. Aluko, Food Chem., 255, 216 (2018); https://doi.org/10.1016/j.foodchem.2018.02.068
Q. Zhang, Z. Cheng, Y. Wang and L. Fu, Crit. Rev. Food Sci. Nutr., 61, 3589 (2021); https://doi.org/10.1080/10408398.2020.1803199
F.U. Akharume, R.E. Aluko and A.A. Adedeji, Compr. Rev. Food Sci. Food Saf., 20, 198 (2021); https://doi.org/10.1111/1541-4337.12688
D. Tazeddinova, M.R. Rahman, S.B. Hamdan, M.M. Matin, M.B. Bakri and M.M. Rahman, BioResources, 17, 7110 (2022).
F. Shahidi and C.S. Dissanayaka, Food Prod. Process. Nutr., 5, 2 (2023); https://doi.org/10.1186/s43014-022-00121-0
H. Yilmaz, B. Gultekin Subasi, H.U. Celebioglu, T. Ozdal and E. Capanoglu, Front. Nutr., 9, 914118 (2022); https://doi.org/10.3389/fnut.2022.914118
K. Schild, F.D. Sönnichsen, D. Martin, V.M. Garamus, A.J. Van der Goot, K. Schwarz and J.K. Keppler, Food Chem., 426, 136496 (2023); https://doi.org/10.1016/j.foodchem.2023.136496
E. Torres-Sánchez, B. Hernández-Ledesma and L.F. Gutiérrez, Foods, 12, 2401 (2023); https://doi.org/10.3390/foods12122401
J.T. Lembe, Ph.D. Thesis, Hemi-synthesis and Biological Activity of Naturally Occurring Compounds from Two Cameroonian Medical Plants: Hypodaphnis zenkeri Engl. Stapf. (Lauraceae) and Staudtia kamerunensis warb. (Myristicaceae), University of Johannesburg, South Africa (2024).