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Identification and Quantification of Germacrene D from the Extracts of Zanthoxylum ovalifolium Leaves by AgNO3-TLC Densitometric Method
Corresponding Author(s) : S. Ravi Kiran
Asian Journal of Chemistry,
Vol. 31 No. 8 (2019): Vol 31 Issue 8
Abstract
Germacrene D an important sesquiterpenoid has been extracted from leaves of Zanthoxylum ovalifoilum and high performance thin-layer chromatographic method has been developed for its simultaneous identification and determination. The compound was extracted by cold extraction and separated and identified on a AgNO3 impregnated silica gel 60 F254 HPTLC plates with dichloromethane:acetone (9.8:0.2 v/v) as mobile phase. The quantification of geremcare D was carried out by densitometry scanning performed at λ = 254 nm by reflectance scanning. This facilitated a well-resolved band for the compound with a Rf value of 0.46 ± 0.01. Linear regression concentration analysis data for mean calibration plots showed good linear relationship in the range of 1000-10000 ng/mL, with a correlation coefficient, slope and intercept of 0.9985 ± 0.0004, 21.62 ± 0.435 and 3308.87 ± 10.24, respectively. The minimum amount of germacrene D that could be authentically detected (LOD) and quantified (LOQ) were determined and found to be 212.07 and 521.23 ng/spot, respectively. The method was validated by considering the parameters linearity, precision, accuracy, specificity and robustness wherein, it is evident from the results that the proposed method was simple, safe and reliable.
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- B.A. McAndrew, Perfumes: Art, Science and Technology, Elsevier Applied Science: London, New York (1992).
- K. Yoshihara, Y. Ohta, T. Sakai and Y. Hirose, Tetrahedron Lett., 10, 2263 (1969); https://doi.org/10.1016/S0040-4039(01)88136-3.
- N. Bulow and W.A. Konig, Phytochemistry, 55, 141 (2000); https://doi.org/10.1016/S0031-9422(00)00266-1.
- M. Telascrea, C.C. de Araújo, M.O.M. Marques, R. Facanali, P.L.R. de Moraes and A.J. Cavalheiro, Biochem. Syst. Ecol., 35, 222 (2007); https://doi.org/10.1016/j.bse.2006.09.015.
- C. Nishino, W.S. Bowers, M.E. Montgomery, L.R. Nault and M.W. Nielson, J. Chem. Ecol., 3, 349 (1977); https://doi.org/10.1007/BF00988450.
- S.R. Kiran, K. Bhavani, P.S. Devi, B.R. Rajeswara Rao and K.J. Reddy, Bioresour. Technol., 97, 2481 (2006); https://doi.org/10.1016/j.biortech.2005.10.003.
- S.R. Kiran, A.S. Reddy, P.S. Devi and K.J. Reddy, Pest Manag. Sci., 62, 1116 (2006); https://doi.org/10.1002/ps.1266.
- S.R. Kiran, P.S. Devi and K.J. Reddy, Curr. Sci., 93, 544 (2007).
- P. Pavani, H.S. Ashwini, A. Chittaragi and R. Naika, Int. J. Pharm. Pharm. Res., 3, 323 (2015).
- B. Singh, A.K. Uniyal and N.P. Todaria, J. Sustain. Agric., 30, 87 (2007); https://doi.org/10.1300/J064v30n03_07.
- O. Quattrochi, S. Abelaira and R. Laba, Introducción a la HPLC. Impreso En La Argentina, Buenos Aires (1992).
- W. Daniel, Bioestadística, Base para el análisis de las ciencias de la salud, Uteha Noriega Editores, México (1996).
- The United States Pharmacopeia/The National Formulary (USP 25/ NF 20), United States Pharmacopeial Convection Inc., USA (2002).
- ICH Harmonized Tripartite Guideline, Text on Validation of Analytical Procedures (1994).
- ICH Harmonized Tripartite Guideline, Guidance on Analytical Method Validation, in: Proceedings of the International Convention on Quality for the Pharmaceutical Industry, Toronto, Canada (2002).
References
B.A. McAndrew, Perfumes: Art, Science and Technology, Elsevier Applied Science: London, New York (1992).
K. Yoshihara, Y. Ohta, T. Sakai and Y. Hirose, Tetrahedron Lett., 10, 2263 (1969); https://doi.org/10.1016/S0040-4039(01)88136-3.
N. Bulow and W.A. Konig, Phytochemistry, 55, 141 (2000); https://doi.org/10.1016/S0031-9422(00)00266-1.
M. Telascrea, C.C. de Araújo, M.O.M. Marques, R. Facanali, P.L.R. de Moraes and A.J. Cavalheiro, Biochem. Syst. Ecol., 35, 222 (2007); https://doi.org/10.1016/j.bse.2006.09.015.
C. Nishino, W.S. Bowers, M.E. Montgomery, L.R. Nault and M.W. Nielson, J. Chem. Ecol., 3, 349 (1977); https://doi.org/10.1007/BF00988450.
S.R. Kiran, K. Bhavani, P.S. Devi, B.R. Rajeswara Rao and K.J. Reddy, Bioresour. Technol., 97, 2481 (2006); https://doi.org/10.1016/j.biortech.2005.10.003.
S.R. Kiran, A.S. Reddy, P.S. Devi and K.J. Reddy, Pest Manag. Sci., 62, 1116 (2006); https://doi.org/10.1002/ps.1266.
S.R. Kiran, P.S. Devi and K.J. Reddy, Curr. Sci., 93, 544 (2007).
P. Pavani, H.S. Ashwini, A. Chittaragi and R. Naika, Int. J. Pharm. Pharm. Res., 3, 323 (2015).
B. Singh, A.K. Uniyal and N.P. Todaria, J. Sustain. Agric., 30, 87 (2007); https://doi.org/10.1300/J064v30n03_07.
O. Quattrochi, S. Abelaira and R. Laba, Introducción a la HPLC. Impreso En La Argentina, Buenos Aires (1992).
W. Daniel, Bioestadística, Base para el análisis de las ciencias de la salud, Uteha Noriega Editores, México (1996).
The United States Pharmacopeia/The National Formulary (USP 25/ NF 20), United States Pharmacopeial Convection Inc., USA (2002).
ICH Harmonized Tripartite Guideline, Text on Validation of Analytical Procedures (1994).
ICH Harmonized Tripartite Guideline, Guidance on Analytical Method Validation, in: Proceedings of the International Convention on Quality for the Pharmaceutical Industry, Toronto, Canada (2002).