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Vol. 37 No. 11 (2025): Vol 37 Issue 11, 2025

Copyright (c) 2025 Sumitra Pandey, A.K. Sarkar, Ritu Chauhan

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Comparative Studies of Antioxidant Activities of Herbal Formulation of Curcuma longa L. and Cannabis sativa L. Plant Extracts: A Phytochemical Approach

https://doi.org/10.14233/ajchem.2025.34350
Sumitra Pandey
Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492015, India
A.K. Sarkar
Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492015, India
Ritu Chauhan
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun-248002, India
https://orcid.org/0000-0002-9056-6619

Corresponding Author(s) : A.K. Sarkar

ashisk.sarkar@sruraipur.ac.in

Asian Journal of Chemistry, Vol. 37 No. 11 (2025): Vol 37 Issue 11, 2025

Abstract

The present study investigates the comparative antioxidant potential of Curcuma longa L. and Cannabis sativa L. extracts, individually and in combination, with the aim of developing novel herbal formulations. Multiple extraction techniques were employed for C. longa, including aqueous, heated, ethanol and Soxhlet methods, while C. sativa extracts were obtained via hydro-distillation of essential oil and cold pressing of hemp seed oil. Phytochemical screening confirmed the presence of phenolics, flavonoids, alkaloids, curcuminoids, terpenoids, cannabinoids and fatty acids, which are known contributors to antioxidant and antimicrobial activities. Characterization using UV-vis, TLC, HPLC, FTIR and GC-MS validated the presence of key functional groups and bioactive metabolites. Antioxidant capacity was determined using the DPPH radical scavenging assay, with ascorbic acid serving as the standard (IC50 = 12.68 µg/mL). Results indicated that C. longa ethanolic extract exhibited strong radical scavenging activity (IC50 = 21.01 µg/mL; 85.2% inhibition at 320 µg/mL), whereas C. sativa hemp seed oil showed weak activity (maximum 10.01% inhibition at 320 mg/mL; IC50 > 320 µg/mL) and essential oil demonstrated moderate potential. Importantly, the combined formulation of C. longa ethanolic extract with C. sativa essential oil displayed significantly enhanced antioxidant capacity (up to 85.2% scavenging at 100 µg/mL with an IC50 of 45.6 µg/mL), suggesting synergistic interactions among phytoconstituents. GC-MS profiling further revealed that C. longa ethanolic extract was rich in dehydrozingerone (35.82%), turmerones (18.29%) and curlone (8.60%), whereas hemp seed oil was dominated by linoleic acid (56.11%), α-linolenic acid (17.02%) and oleic acid (13.64%), reflecting a complementary balance of phenolic antioxidants and polyunsaturated fatty acids. These findings emphasize the chemical and biological complementarity of turmeric and cannabis, supporting their integration in polyherbal formulations aimed at mitigating oxidative stress. The study highlights the potential of turmeric–cannabis combinations as natural alternatives for antioxidant therapy, providing a basis for further pharmacological and nutraceutical applications.

Keywords

Curcuma longa Cannabis sativa Phytochemical analysis Antioxidant activity Herbal formulation
(1)
Pandey, S.; Sarkar, A.; Chauhan, R. Comparative Studies of Antioxidant Activities of Herbal Formulation of Curcuma Longa L. And Cannabis Sativa L. Plant Extracts: A Phytochemical Approach. ajc 2025, 37, 2846-2854.
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