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Vol. 34 No. 8 (2022): Vol 34 Issue 8, 2022

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Ethanol and Acetic Acid Induces Conformational Changes in Zebrafish Dihydrofolate Reductase Protein

https://doi.org/10.14233/ajchem.2022.23734
V.V. Acharya
Molecular Biophysics Lab, Amity Institute of Biotechnology, Amity University, Sector 125, Noida-201313, India
A.K. Verma
Department of Zoology, Kirori Mal College, University of Delhi, New Delhi-110007, India
P. Chaudhuri (Chattopadhyay)
Molecular Biophysics Lab, Amity Institute of Biotechnology, Amity University, Sector 125, Noida-201313, India

Corresponding Author(s) : P. Chaudhuri (Chattopadhyay)

pchaudhuri@amity.edu

Asian Journal of Chemistry, Vol. 34 No. 8 (2022): Vol 34 Issue 8, 2022

Abstract

An equilibrium unfolding research of the recombinant zDHFR would provide information on the conformational changes of protein. In present work, the conformation of recombinant zDHFR was investigated in presence of ethanol and acetic acid. Equilibrium unfolding of recombinant zDHFR was monitored by enzymatic assay after denaturation by ethanol and acetic acid at 340 nm. Changes in secondary and tertiary structure of zDHFR with increasing ethanol and acetic acid concentrations were investigated in far-UV circular dichroism (CD) (190 to 250 nm) and fluorescence spectroscopy (emission spectra from 300 to 400 nm with an excitation wavelength of 295 nm) methods. It has been observed that in case of acetic acid-induced denaturation of zDHFR, the shift from native to denatured state occurs in a single step, whereas intermediates or non-native states are found at low concentrations of ethanol. The recent findings have significant implications for understanding how ethanol and acetic acid affect protein structure.

Keywords

Acetic acid CD spectroscopy Ethanol Intrinsic fluorescence Protein unfolding zDHFR
Acharya, V., Verma, A., & Chaudhuri (Chattopadhyay), P. (2022). Ethanol and Acetic Acid Induces Conformational Changes in Zebrafish Dihydrofolate Reductase Protein. Asian Journal of Chemistry, 34(8), 2178–2182. https://doi.org/10.14233/ajchem.2022.23734
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References
  1. C.N. Pace, J.M. Scholtz and G.R. Grimsley, FEBS Lett., 588, 2177 (2014); https://doi.org/10.1016/j.febslet.2014.05.006
  2. E.J. Milner-White, Interface Focus, 9, 20190057 (2019); https://doi.org/10.1098/rsfs.2019.0057
  3. M. Dorn, M.B. Silva, L.S. Buriol and L.C. Lamb, Computat. Biol. Chem., 53, 251 (2014); https://doi.org/10.1016/j.compbiolchem.2014.10.001
  4. R. Bhattacharya, P.W. Rose, S.K. Burley and A. Prlic, PLoS ONE, 12, e0171355 (2017); https://doi.org/10.1371/journal.pone.0171355
  5. M. Arai, Biophys. Rev., 10, 163 (2018); https://doi.org/10.1007/s12551-017-0346-7
  6. L.J. Lapidus, F1000 Res., 6, 1723 (2017); https://doi.org/10.12688/f1000research.12070.1
  7. V.A. Risso and M.R. Ermácora, Eur. Biophys. J., 48, 341 (2019); https://doi.org/10.1007/s00249-019-01361-8
  8. C.L. Tooke, P. Hinchliffe, E.C. Bragginton, C.K. Colenso, V. Hirvonen, Y. Takebayashi and J. Spencer, J. Mol. Biol., 431, 3472 (2019); https://doi.org/10.1016/j.jmb.2019.04.002
  9. D.N. Brems, S.M. Plaisted, H.A. Havel, E.W. Kauffman, J.D. Stodola, L.C. Eaton and R.D. White, Biochemistry, 24, 7662 (1985); https://doi.org/10.1021/bi00347a025
  10. M. Sukumar, S. Storms and M. De Felippis, Pharm. Res., 22, 789 (2005); https://doi.org/10.1007/s11095-005-2596-5
  11. J. Lamba, S. Paul, V. Hasija, R. Aggarwal and T.K. Chaudhuri, Biochemistry (Moscow), 74, 393 (2009); https://doi.org/10.1134/S000629790904006
  12. A. Hagarman, L. Duitch and R. Schweitzer-Stenner, Biochemistry, 47, 9667 (2008); https://doi.org/10.1021/bi800729w
  13. P.F. Faísca, A. Nunes, R.D. Travasso and E.I. Shakhnovich, Protein Sci., 19, 2196 (2010); https://doi.org/10.1002/pro.498
  14. Y. Gambin, M. Polinkovsky, B. Francois, N. Giles, A. Bhumkar and E. Sierecki, Int. J. Mol. Sci., 17, 655 (2016); https://doi.org/10.3390/ijms17050655
  15. F. Chiti and C.M. Dobson, Annu. Rev. Biochem., 86, 27 (2006); https://doi.org/10.1146/annurev.biochem.75.101304.123901
  16. A. Lau, M. Bourkas, Y. Lu, L.A. Ostrowski, D. Weber-Adrian, C. Figueiredo, H. Arshad, S. Shoaei, C.D. Morrone, S. Matan-Lithwick, K.J. Abraham, H. Wang and G. Schmitt-Ulms, Discoveries, 5, e79 (2017); https://doi.org/10.15190/d.2017.9
  17. Z.L. Almeida and R.M.M. Brito, Molecules, 25, 1195 (2020); https://doi.org/10.3390/molecules25051195
  18. R. Liu, P. Qin, L. Wang, X. Zhao, Y. Liu and X. Hao, J. Biochem. Mol. Toxicol., 66-71 (2010); https://doi.org/10.1002/jbt.20314
  19. T.T. Kao, K.C. Wang, W.N. Chang, C.Y. Lin, B.H. Chen, H.L. Wu, G.Y. Shi, J.N. Tsai and T.F. Fu, Drug Metab. Dispos., 36, 508 (2008); https://doi.org/10.1124/dmd.107.019299
  20. B.L. Hillcoat, P.F. Nixon and R.L. Blakley, Anal. Biochem., 21, 178 (1967); https://doi.org/10.1016/0003-2697(67)90179-0
  21. C. Thapliyal, N. Jain and P.C. Chattopadhyay, Int. J. Biol. Macromol., 91, 736 (2016); https://doi.org/10.1016/j.ijbiomac.2016.06.017
  22. K. Kuwajima, Biomolecules, 10, 407 (2020); https://doi.org/10.3390/biom10030407
  23. P.J. Guyett and L.M. Gloss, Comprehensive Biophysics, 43-71 (2012); https://doi.org/10.1016/B978-0-12-374920-8.00304-0
  24. S Prajapati, European J. Biochem., 178-84 (1998); https://doi.org/10.1046/j.1432-1327.1998.2550178.x
  25. H. Chen and E. Rhoades, Curr. Opin. Struct. Biol., 516-24 (2008); https://doi.org/10.1016/j.sbi.2008.06.008
  26. A.C. Ferreon and D.W. Bolen, Biochemistry, 43, 13357 (2004); https://doi.org/10.1021/bi048666j
  27. R.H. Khan, S. Rasheedi and S. Haq, J. Biosci., 28, 709 (2003); https://doi.org/10.1007/BF02708431
  28. N. Nikolaidis, M. Andreadis and T. Moschakis, Food Chem., 1, 425 (2017); https://doi.org/10.1016/j.foodchem.2017.04.022
  29. G. Shanmugam, C.C. Selvi and A.B. Mandal, Int. J. Biol. Macromol., 51, 590 (2012); https://doi.org/10.1016/j.ijbiomac.2012.06.026
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