Issue

Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

Copyright (c) 2022 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

GC-MS Analysis, HPTLC Fingerprint Profile and Antidiabetic Activity of Methanolic Extract of Perilla frutescens Leaves

https://doi.org/10.14233/ajchem.2022.23869
Arunesh Kumar Dixit
Department of Chemistry, School of Applied and Life Sciences, Uttaranchal University, Arcadia Grant, Premnagar, Dehradun-248007, India
Ajay Singh
Department of Chemistry, School of Applied and Life Sciences, Uttaranchal University, Arcadia Grant, Premnagar, Dehradun-248007, India
S. Farooq
Himalaya Wellness Company, Saharanpur Road, Clement Town, Dehradun-248002, India
Umesh Kumar Dixit
Department of Biochemistry, I.T.S. Dental College, Delhi- Meerut Road, Murad Nagar, Ghaziabad-201206, India
Zafar Mehmood
Himalaya Wellness Company, Saharanpur Road, Clement Town, Dehradun-248002, India

Corresponding Author(s) : Arunesh Kumar Dixit

dixit.arunesh@gmail.com

Asian Journal of Chemistry, Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

Abstract

The present study was aimed to investigate the phytochemical constituents of the different extracts of Perilla frutescens leaves and identification of the compounds by subjecting it to HPTLC and GC-MS analysis along with antidiabetic property of leaves of P. frutescens. The results from HPTLC finger print scanned at wavelength 200 nm for methanolic extract of P. frutescens leaf revealed the presence of seventeen polyvalent phytoconstituents. The HPTLC finger print scanned at wavelength 200 nm for ethanolic extract of P. frutescens leaves showed fifteen polyvalent phytoconstituents and corresponding ascending order of Rf values ranged from 0.02 to 0.78 in which highest concentration of the phytoconstituents was found to be 13.45 % and its corresponding Rf value was found to be 0.64. α-Amylase inhibition of methanolic extract of P. frutescens leaves was also performed. The standard (acarbose 0.5-2.0 mg/mL) revealed greatest α-amylase inhibitory action from 43.82 ± 0.12 to 88.14 ± 0.32% with IC50 value worth of 0.575 mg/mL. At a similar focus methanol concentrates of P. frutescens leaves showed the inhibitory action from 28.21 ± 0.11% to 68.01 ± 0.11%, with an IC50 worth of 1.15 mg/mL. The methanolic extract of P. frutescens leaves exhibited inhibition of α-amylase enzyme proved the antidiabetic potentiality of P. frutescens leaves.

Keywords

Perilla frutescens Antidiabetic activity α-Amylase inhibitors HPTLC GC-MS
Kumar Dixit, A., Singh, A., Farooq, S., Kumar Dixit, U., & Mehmood, Z. (2022). GC-MS Analysis, HPTLC Fingerprint Profile and Antidiabetic Activity of Methanolic Extract of Perilla frutescens Leaves. Asian Journal of Chemistry, 34(7), 1875–1880. https://doi.org/10.14233/ajchem.2022.23869
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. D. Dias, S. Urban and U. Roessner, Metabolites, 2, 303 (2012); https://doi.org/10.3390/metabo2020303
  2. L. Chen, T. Huang, J. Zhang, M.-Y. Zheng, K.-Y. Feng, Y.-D. Cai and K.-C. Chou, BioMed Res. Int., 2013, 485034 (2013); https://doi.org/10.1155/2013/485034
  3. N. Sahoo and P. Manchikanti, J. Altern. Complement. Med., 19, 957 (2013); https://doi.org/10.1089/acm.2012.0275
  4. H. Yuan, Q. Ma, L. Ye and G. Piao, Molecules, 21, 559 (2016); https://doi.org/10.3390/molecules21050559
  5. G. Daryabor, M.R. Atashzar, D. Kabelitz, S. Meri and K. Kalantar, Front. Immunol., 11, 1582 (2020); https://doi.org/10.3389/fimmu.2020.01582
  6. I. Hameed, S.R. Masoodi, S.A. Mir, M. Nabi, K. Ghazanfar and B.A. Ganai, World J. Diabetes, 6, 598 (2015); https://doi.org/10.4239/wjd.v6.i4.598
  7. A. Chawla, R. Chawla and S. Jaggi, Indian J. Endocrinol. Metab., 20, 546 (2016); https://doi.org/10.4103/2230-8210.183480
  8. K. Ogurtsova, J.D. da Rocha Fernandes, Y. Huang, U. Linnenkamp, L Guariguata, N.H. Cho, D Cavan, J.E. Shaw and L.E. Makaroff, Diabetes Res. Clin. Pract., 128, 40 (2017); https://doi.org/10.1016/j.diabres.2017.03.024
  9. A. Kumar, M.K. Goel, R.B. Jain, P. Khanna and V. Chaudhary, Australas. Med. J., 6, 524 (2013); https://doi.org/10.4066/AMJ.2013.1791
  10. C. Bailey and M. Kodack, Int. J. Clin. Pract., 65, 314 (2011); https://doi.org/10.1111/j.1742-1241.2010.02544.x
  11. R. Ortiz-Andrade, V. Rodríguez-López, M. Garduño-Ramírez, P. CastilloEspaña and S. Estrada-Soto, J. Ethnopharmacol., 101, 37 (2005); https://doi.org/10.1016/j.jep.2005.03.022
  12. W. Parys, M. Dolowy and A. Pyka-Pajak, Processes, 10, 172 (2022); https://doi.org/10.3390/pr10010172
  13. K. Anjoo and S.A. Kumar, Pharmacogn. J., 2, 254 (2010); https://doi.org/10.1016/S0975-3575(10)80113-0
  14. H.N. Pravin, K. Joseph, J. Aruna and K. Vilasrao, J. Appl. Pharm. Sci., 2, 38 (2012).
  15. G. Cragg and D. Newman, Biochim. Biophys. Acta, 1830, 3670 (2013); https://doi.org/10.1016/j.bbagen.2013.02.008
  16. A. Dhyani, R. Chopra and M. Garg, Biomed. Pharmacol. J., 12, 649 (2019); https://doi.org/10.13005/bpj/1685
  17. N. Dhami, Ethnomedicinal Uses of Plants is Western Terai of Nepal: A Case Study of Dekhatbhuli VDC of Kanchanpur District; In: Medicinal Plants in Nepal: An Anthology of Contemporary Research, Ecological Society; Kathmandu, Nepal, pp. 165-177 (2008).
  18. M. Asif, Avicenna J. Phytomed., 2, 169 (2012).
  19. R. Dimita, S.M. Allah, A. Luvisi, D. Greco, L. De Bellis, R. Accogli, C. Mininni and C. Negro, Horticulturae, 8, 71 (2022); https://doi.org/10.3390/horticulturae8010071
  20. H.M. Ahmed, Molecules, 24, 102 (2019); https://doi.org/10.3390/molecules24010102
  21. G. Zurera, B. Estrada, F. Rincón and R. Pozo, Bull. Environ. Contam. Toxicol., 38, 805 (1987); https://doi.org/10.1007/BF01616705
  22. E. Reich and A. Schibli, High-Performance Thin Layer Chromatography for the Analysis of Medicinal Plants, Thieme Medical Publisher, Inc.: New York, Ed. 1 (2007).
  23. Z. Xiao, R. Storms and A. Tsang, Anal. Biochem., 351, 146 (2006); https://doi.org/10.1016/j.ab.2006.01.036
  24. G.L. Miller, Anal. Chem., 31, 426 (1959); https://doi.org/10.1021/ac60147a030
  25. Y. Hara and M. Honda, Agric. Biol. Chem., 54, 1939 (1990); https://doi.org/10.1080/00021369.1990.10870239
  26. V. Aparna, K.V. Dileep, P.K. Mandal, P. Karthe, C. Sadasivan and M. Haridas, Chem. Biol. Drug Des., 80, 434 (2012); https://doi.org/10.1111/j.1747-0285.2012.01418.x
  27. P.P. Kumar, S. Kumaravel and C. Lalitha, Afr. J. Biochem. Res., 4, 191 (2010).
  28. A.A. Rahuman, G. Gopalakrishnan, B.S. Ghouse, S. Arumugam and B. Himalayan, Fitoterapia, 71, 553 (2000); https://doi.org/10.1016/S0367-326X(00)00164-7
  29. Z. Xiao, R. Storms and A. Tsang, Anal. Biochem., 351, 146 (2006); https://doi.org/10.1016/j.ab.2006.01.036
  30. D. Saint-Leger, A. Bague, E. Cohen and M. Lchivot, Br. J. Dermatol., 114, 535 (1986); https://doi.org/10.1111/j.1365-2133.1986.tb04060.x
  31. S. Senthilkumar, T. Devaki, B.M. Manohar and M.S. Babu, Clin. Chim. Acta, 364, 335 (2006); https://doi.org/10.1016/j.cca.2005.07.032
  32. A. Mitra, M. Manjunatha and B. Dineshkumar, Int. J. Green Pharm., 4, 115 (2010); https://doi.org/10.4103/0973-8258.63887
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0