Issue

Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

Copyright (c) 2022 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Silver Nanoparticles Mediated through Aqueous Leaf Extract of Ageratina adenophora Inhibit Proliferation and Induce Apoptosis in Human Ovarian Teratocarcinoma Cell Line PA-1

https://doi.org/10.14233/ajchem.2022.23787
B. Latha Maheswari
PG and Research Department of Chemistry, A.V.V.M. Sri Pushpam College (Affiliated to Bharathidasan University), Poondi-613503, India
N. Mani
PG and Research Department of Chemistry, A.V.V.M. Sri Pushpam College (Affiliated to Bharathidasan University), Poondi-613503, India
S. Karthika
PG and Research Department of Chemistry, A.V.V.M. Sri Pushpam College (Affiliated to Bharathidasan University), Poondi-613503, India
N. Kavikala
PG and Research Department of Chemistry, A.V.V.M. Sri Pushpam College (Affiliated to Bharathidasan University), Poondi-613503, India

Corresponding Author(s) : B. Latha Maheswari

rajilatha54@gmail.com

Asian Journal of Chemistry, Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

Abstract

An enhanced anticancer properties of Ageratina adenophora mediated silver nanoparticles (AgNPs) were evaluated in the present study. The biogenic AgNPs effectively inhibited the viability and proliferation of ovarian teratocarcinoma cells (PA-1) by significantly increasing the initiation of apoptosis. The AgNPs were synthesized with the phytochemicals present in aqueous extract of Ageratina adenophora leaves which performed the dual function of reducing silver ions as well as the capping the nanoparticles. Characterization of nanoparticles with UV-vis spectroscopy revealed surface plasmon resonance peak at 470 nm. Fourier transform infrared spectrum showed the presence of functional groups such as alcohol, aldehyde, alkane, aromatic amines and phenolic compounds. X-Ray diffraction (XRD) analysis revealed the crystalline nature and face centred cubic structure of AgNPs. The size, morphology and distribution of AgNPs were confirmed with scanning electron microscope (SEM). Further, dynamic light scattering (DLS) and zeta potential measurements also confirmed the size and charge of the synthesized nanoparticles. The AgNPs significantly increased the cytotoxicity and inhibited the proliferation of PA-1 cells. Apoptosis of PA-1 cells were increased with treatment of AgNPs. The increased cytotoxicity of PA-1 cells was due to the synergistic activity of phytochemicals and AgNPs. Hence, the current research on the silver nanoparticles mediated through A. adenophora leaf extract could be an effective alternative in treatment of ovarian cancer.

Keywords

Ageratina adenophora Silver nanoparticles PA-1 cells Ovarian cancer Apoptosis
Latha Maheswari, B., Mani, N., Karthika, S., & Kavikala, N. (2022). Silver Nanoparticles Mediated through Aqueous Leaf Extract of Ageratina adenophora Inhibit Proliferation and Induce Apoptosis in Human Ovarian Teratocarcinoma Cell Line PA-1. Asian Journal of Chemistry, 34(7), 1755–1762. https://doi.org/10.14233/ajchem.2022.23787
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. M.E.A. El-Hack, S. Abdelnour, M. Alagawany, M. Abdo, M.A. Sakr, A.F. Khafaga, S.A. Mahgoub, S.S. Elnesr and M.G. Gebriel, Biomed. Pharmacother., 111, 42 (2019); https://doi.org/10.1016/j.biopha.2018.12.069
  2. S.H. Hassanpour and M. Dehghani, J. Cancer Res. Pract., 4, 127 (2017); https://doi.org/10.1016/j.jcrpr.2017.07.001
  3. C.L. Sutton, C.D. McKinney, J.E. Jones and S.B. Gay, Radiographics, 12, 853 (1992); https://doi.org/10.1148/radiographics.12.5.1529129
  4. Z. Momenimovahed, A. Tiznobaik, S. Taheri and H. Salehiniya, Int. J. Womens Health, 11, 287 (2019); https://doi.org/10.2147/IJWH.S197604
  5. N.S. Murthy, S. Shalini, G. Suman, S. Pruthvish and A. Mathew, Asian Pac. J. Cancer Prev., 10, 1025 (2009).
  6. F. Bray, J. Ferlay, I. Soerjomataram, R.L. Siegel, L.A. Torre and A. Jemal, CA Cancer J. Clin., 68, 394 (2018); https://doi.org/10.3322/caac.21492
  7. R. Foster, R.J. Buckanovich and B.R. Rueda, Cancer Lett., 338, 147 (2013); https://doi.org/10.1016/j.canlet.2012.10.023
  8. J. Swanner, J. Mims, D.L. Carroll, S.A. Akman, C.M. Furdui, S.V. Torti and R.N. Singh, Int. J. Nanomedicine, 10, 3937 (2015); https://doi.org/10.2147/IJN.S80349
  9. Q. Yue, G. Gao, G. Zou, H. Yu and X. Zheng, BioMed Res. Int., 2017, 8412508 (2017); https://doi.org/10.1155/2017/8412508
  10. S.Y. Yin, W.C. Wei, F.Y. Jian and N.S. Yang, Evid. Based Complement. Alternat. Med., 2013, 302426 (2013); https://doi.org/10.1155/2013/302426
  11. D.O. Kennedy and E.L. Wightman, Adv. Nutr., 2, 32 (2011); https://doi.org/10.3945/an.110.000117
  12. D.W. Nyamai, W. Arika, P. Ogola, E. Njagi and M. Ngugi, J. Pharmacogn. Phytochem., 4, 2321 (2016).
  13. Y. Qiansheng, Y. Jie, L. Huamin, C. Aocheng, C. Qinghua, W. Yongqi and H. Lan, J. Beijing Normal Univ. Nat. Sci. Ed., 42, 70 (2006).
  14. L. He, J. Hou, M. Gan, J. Shi, S. Chantrapromma, H.K. Fun, I.D.
  15. Williams and H.H.-Y. Sung, J. Nat. Prod., 71, 1485 (2008); https://doi.org/10.1021/np800242w
  16. V. Ahluwalia, R. Sisodia, S. Walia, O.P. Sati, J. Kumar and A. Kundu, J. Pest Sci., 87, 341 (2014); https://doi.org/10.1007/s10340-013-0542-6
  17. C. Wang, R. Yang, L. Song, B. Ning, C. Ou-yang, A. Cao and L. He, Phytochem. Lett., 16, 245 (2016); https://doi.org/10.1016/j.phytol.2016.04.002
  18. N. Ramya, P.K. Mayuri, A.S. Santny and T. Angayarkanni, Asia Pacific J. Res., 1, 170 (2015).
  19. L. Samuel, Lalrotluanga, R.B. Muthukumaran, G. Gurusubramanian and N. Senthilkumar, Exp. Parasitol., 141, 112 (2014); https://doi.org/10.1016/j.exppara.2014.03.020
  20. A.K. Chakravarty, T. Mazumder and S.N. Chatterjee, Evid. Based Complement. Alternat. Med., 2011, 471074 (2011); https://doi.org/10.1093/ecam/neq033
  21. C.L. Ringmichon and B. Gopalkrishnan, Int. J. Appl. Biol. Pharm. Technol., 8, 1 (2017).
  22. V. Ralte and S. Lallianrawna, Sci. Vision, 14, 128 (2014).
  23. S.K. Mandal, R. Boominathan, B. Parimaladevi, S. Dewanjee and S.C. Mandal, Indian J. Exp. Biol., 43, 662 (2005).
  24. H. Chen, B. Zhou, J. Yang, X. Ma, S. Deng, Y. Huang, Y. Wen, J. Yuan and X. Yang, Front. Pharmacol., 9, 483 (2018); https://doi.org/10.3389/fphar.2018.00483
  25. Y.-S. Li, H. Zou, N. Zhu, W. Li, X.-Y. Na, S.-Z. Tang and Y.Z. Yang, J. Southwest Agric. Univ., 22, 331 (2000).
  26. Y. Jin, L. Hou, M. Zhang, Z. Tian, A. Cao and X. Xie, Crop Prot., 60, 28 (2014); https://doi.org/10.1016/j.cropro.2014.02.008
  27. S. Mani, K. Natesan, K. Shivaji, M.G. Balasubramanian and P. Ponnusamy, Biocatal. Agric. Biotechnol., 22, 101381 (2019); https://doi.org/10.1016/j.bcab.2019.101381
  28. S. Hemmati, A. Rashtiani, M.M. Zangeneh, P. Mohammadi, A. Zangeneh and H. Veisi, Polyhedron, 158, 8 (2019); https://doi.org/10.1016/j.poly.2018.10.049
  29. A.R. Jalalvand, M. Zhaleh, S. Goorani, M.M. Zangeneh, N. Seydi, A. Zangeneh and R. Moradi, J. Photochem. Photobiol. B, 192, 103 (2019); https://doi.org/10.1016/j.jphotobiol.2019.01.017
  30. M. Look, A. Bandyopadhyay, J.S. Blum and T.M. Fahmy, Adv. Drug Deliv. Rev., 62, 378 (2010); https://doi.org/10.1016/j.addr.2009.11.011
  31. A. Zangeneh, M. Pooyanmehr, M.M. Zangeneh, R. Moradi, R. Rasad and N. Kazemi, Comp. Clin. Pathol., 28, 1507 (2019); https://doi.org/10.1007/s00580-019-03007-9
  32. M.M. Zangeneh, A. Zangeneh, E. Pirabbasi, R. Moradi and M. Almasi, Appl. Organomet. Chem., 33, e5246 (2019); https://doi.org/10.1002/aoc.5246
  33. S. Goorani, M.K. Koohi, H. Morovvati, J. Hassan, A. Ahmeda and M.M. Zangeneh, Appl. Organomet. Chem., 34, e5475 (2020); https://doi.org/10.1002/aoc.5475
  34. S. Hemmati, Z. Joshani, A. Zangeneh and M.M. Zangeneh, Appl. Organomet. Chem., 34, e5277 (2020); https://doi.org/10.1002/aoc.5277
  35. M.S. Akhtar, M.K. Swamy, A. Umar and A.A. Al Sahli, J. Nanosci. Nanotechnol., 15, 9818 (2015); https://doi.org/10.1166/jnn.2015.10966
  36. G.R. Rudramurthy, M.K. Swamy, U.R. Sinniah and A. Ghasemzadeh, Molecules, 21, 836 (2016); https://doi.org/10.3390/molecules21070836
  37. A.K. Mittal, K. Thanki, S. Jain and U.C. Banerjee, Appl. Nanomed., 1, 1 (2016).
  38. K. Elangovan, D. Elumalai, S. Anupriya, R. Shenbhagaraman, P.K. Kaleena and K. Murugesan, J. Photochem. Photobiol. B, 151, 118 (2015); https://doi.org/10.1016/j.jphotobiol.2015.05.015
  39. S. Gurunathan, J.W. Han, V. Eppakayala, M. Jeyaraj and J.H. Kim, BioMed Res. Int., 2013, 535796 (2013); https://doi.org/10.1155/2013/535796
  40. S. Gurunathan, J.W. Han, J.H. Park, E. Kim, Y.J. Choi, D.N. Kwon and J.H. Kim, Int. J. Nanomedicine, 10, 6257 (2015); https://doi.org/10.2147/IJN.S92449
  41. S. Gurunathan, J.K. Jeong, J.W. Han, X.F. Zhang, J.H. Park and J.H. Kim, Nanoscale Res. Lett., 10, 35 (2015); https://doi.org/10.1186/s11671-015-0747-0
  42. J.-C. Liou, C.-C. Diao, J.-J. Lin, Y.-L. Chen and C.-F. Yang, Nanoscale Res. Lett., 9, 1 (2014); https://doi.org/10.1186/1556-276X-9-1
  43. S. Sahu, N. Sinha, S.K. Bhutia, M. Majhi and S. Mohapatra, J. Mater. Chem. B Mater. Biol. Med., 2, 3799 (2014); https://doi.org/10.1039/C3TB21669A
  44. C.V. Jagannath and B.K. Radheshyam, World J. Pharm. Pharm. Sci., 1574 (2017); https://doi.org/10.20959/wjpps20174-8945
  45. S.K. Gautam, Y. Baid, P.T. Magar, T.R. Binadi and B. Regmi, Int. J. Appl. Sci. Biotechnol., 9, 128 (2021); https://doi.org/10.3126/ijasbt.v9i2.37822
  46. T. Mosmann, J. Immunol. Methods, 65, 55 (1983); https://doi.org/10.1016/0022-1759(83)90303-4
  47. D. Srivastava, G. Joshi, K. Somasundaram and R. Mulherkar, Anticancer Res., 31, 3851 (2011).
  48. C. Pereira, M.S. Santos and C. Oliveira, Neurobiol. Dis., 6, 209 (1999); https://doi.org/10.1006/nbdi.1999.0241
  49. S.M. Bhosle, N.G. Huilgol and K.P. Mishra, Clin. Chim. Acta, 359, 89 (2005); https://doi.org/10.1016/j.cccn.2005.03.037
  50. D. Baskic, S. Popovic, P. Ristic and N.N. Arsenijevic, Cell Biol. Int., 30, 924 (2006); https://doi.org/10.1016/j.cellbi.2006.06.016
  51. N.A. Franken, H.M. Rodermond, J. Stap, J. Haveman and C. Van Bree, Nat. Protoc., 1, 2315 (2006); https://doi.org/10.1038/nprot.2006.339
  52. N. Ingarsal, V. Kasthuri and S. Ananth, Ann. Rom. Soc. Cell Biol., 25, 2988 (2021).
  53. A.A. Akinsiku, E.O. Dare, K.O. Ajanaku, J.A. Adekoya and J.I. AyoAjayi, J. Mater. Environ. Sci., 3, 902 (2018); https://doi.org/10.26872/jmes.2018.9.3.100
  54. A. León, P. Reuquen, C. Garín, R. Segura, P. Vargas, P. Zapata and P.A. Orihuela, Appl. Sci., 7, 49 (2017); https://doi.org/10.3390/app7010049
  55. Y. Meng, Nanomaterials, 5, 1124 (2015); https://doi.org/10.3390/nano5021124
  56. A. Hossain, Y. Abdallah, M.A. Ali, M.M.I. Masum, B. Li, G. Sun, Y. Meng, Y. Wang and Q. An, Biomolecules, 9, 863 (2019); https://doi.org/10.3390/biom9120863
  57. N. Babu, V.M. Pathak and A. Singh, Pharma Innov., 8, 817 (2019).
  58. A. Gnanasoundari, S. Nagamani and P. Thangamathi, Int. J. Lifesciencs, 3, 127 (2015).
  59. S. Ananth and P. Thangamathi, Int. J. Biol. Sci., 8, 20 (2018).
  60. S.J. Joshi, S.J. Geetha, S. Al-Mamari and A. Al-Azkawi, Jundishapur J. Nat. Pharm. Prod., 13, e67846 (2018); https://doi.org/10.5812/jjnpp.67846
  61. Y.G. Yuan, Q.L. Peng and S. Gurunathan, Int. J. Nanomedicine, 12, 6487 (2017); https://doi.org/10.2147/IJN.S135482
  62. K. Khanra, S. Panja, I. Choudhuri, A. Chakraborty and N. Bhattacharyya, Nano Biomed. Eng., 8, 39 (2016); https://doi.org/10.5101/nbe.v8i1.p39-46
  63. S.R. Palle, J. Penchalaneni, K. Lavudi, S.A. Gaddam, V.S. Kotakadi and V.N. Challagundala, Lett. Appl. Nano Bio. Sci., 9, 1165 (2020); https://doi.org/10.33263/LIANBS93.11651176
  64. K. Cao, J. Yang, C. Lin, B.N. Wang, Y. Yang, J. Zhang, J. Dai, L. Li, C. Nie, Z. Yuan and M. Li, Cancer Biother. Radiopharm., 27, 259 (2012); https://doi.org/10.1089/cbr.2011.1126
  65. S. Gurunathan, J.H. Park, J.W. Han and J.H. Kim, Int. J. Nanomedicine, 10, 4203 (2015); https://doi.org/10.2147/IJN.S83953
  66. Y.G. Yuan, S. Zhang, J.Y. Hwang and I.K. Kong, Oxid. Med. Cell. Longev., 2018, 6121328 (2018); https://doi.org/10.1155/2018/6121328
  67. K. Khanra, S. Panja, I. Choudhuri, A. Chakraborty and N. Bhattacharyya, Nanomed. J., 3, 15 (2016).
  68. M.A. Rathi, Pharmacology, 1, 33 (2018).
  69. J.D. Hayes, A.T. Dinkova-Kostova and K.D. Tew, Cancer Cell, 38, 167 (2020); https://doi.org/10.1016/j.ccell.2020.06.001
  70. C.R. Reczek and N.S. Chandel, Annu. Rev. Cancer Biol., 1, 79 (2017); https://doi.org/10.1146/annurev-cancerbio-041916-065808
  71. J.W. Han, S. Gurunathan, Y.J. Choi and J.H. Kim, Int. J. Nanomedicine, 12, 7529 (2017); https://doi.org/10.2147/IJN.S145147
  72. J.W. Han, J.K. Jeong, S. Gurunathan, Y.J. Choi, J. Das, D.N. Kwon, S.-G. Cho, C. Park, H.G. Seo, J.-K. Park and J.-H. Kim, Nanotoxicology, 10, 361 (2016); https://doi.org/10.3109/17435390.2015.1073396
  73. C.D. Fahrenholtz, J. Swanner, M. Ramirez-Perez and R.N. Singh, J. Nanomater., 2017, 5107485 (2017); https://doi.org/10.1155/2017/5107485
  74. D.C. Chan, Ann. Rev. Pathol.: Mechanisms of disease, 15, 235 (2020); https://doi.org/10.1146/annurev-pathmechdis-012419-032711.
  75. B.C. Dickinson and C.J. Chang, Nat. Chem. Biol., 7, 504 (2011); https://doi.org/10.1038/nchembio.607
  76. H.J. Ahn, K.I. Kim, G. Kim, E. Moon, S.S. Yang and J.S. Lee, PLoS One, 6, 28154 (2011); https://doi.org/10.1371/journal.pone.0028154
  77. T.S. Kamatchi, M.K. Mohamed Subarkhan, R. Ramesh, H. Wang and J.G. Malecki, Dalton Trans., 49, 11385 (2020); https://doi.org/10.1039/D0DT01476A
  78. Y.-J. Choi, J.-H. Park, J. Han, E. Kim, O. Jae-Wook, S. Lee, J.-H. Kim and S. Gurunathan, Int. J. Mol. Sci., 17, 2077 (2016); https://doi.org/10.3390/ijms17122077
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0