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Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

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A Fluorescence and Molecular Docking Study on the Interaction of 4′-Hydroxychalcone with Bovine Serum Albumin and Human Serum Albumin

https://doi.org/10.14233/ajchem.2022.23663
Madhumita Patar
Department of Chemistry, National Institute of Technology Silchar, Cachar-788010, India
Ankita Jalan
Department of Chemistry, National Institute of Technology Silchar, Cachar-788010, India
N. Shaemningwar Moyon
Department of Chemistry, National Institute of Technology Silchar, Cachar-788010, India

Corresponding Author(s) : N. Shaemningwar Moyon

nsmoyon@che.nits.ac.in

Asian Journal of Chemistry, Vol. 34 No. 7 (2022): Vol 34 Issue 7, 2022

Abstract

The interaction of 4′-hydroxychalcone (4′HC) with bovine serum albumin (BSA) and human serum albumin (HSA) was studied under physiological condition (pH=7.0). The fluorescence intensity of both serum albumins was quenched in presence of 4′HC at different temperatures. Stern-Volmer analysis and bimolecular quenching constants indicates the presence of static quenching in BSA. Whereas, fluorescence quenching of HSA is due to both the mechanism of static and dynamic quenching. The formation of ground state complex is further confirmed by absorption spectroscopy. The interaction of 4′HC with BSA is stronger than with HSA. FRET study shows the possible energy transfer between 4′HC with BSA and HSA. The binding site of the protein was identified by molecular docking study. The FTIR and CD analysis indicates conformational change in both the serum albumins. The thermodynamic study indicates that the association of BSA and HSA with 4′HC is spontaneous, enthalpy driver and involves electrostatic force of interaction.

Keywords

4′-Hydroxychalcone Bovine serum albumin Human serum albumin Fluorescence quenching Molecular docking
Patar, M., Jalan, A., & Shaemningwar Moyon, N. (2022). A Fluorescence and Molecular Docking Study on the Interaction of 4′-Hydroxychalcone with Bovine Serum Albumin and Human Serum Albumin. Asian Journal of Chemistry, 34(7), 1711–1722. https://doi.org/10.14233/ajchem.2022.23663
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