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Denatured Crude Protein Extracts from the Sea Anemone Entacmaea quadricolor as Capping Agents for Silver Nanoclusters
Corresponding Author(s) : Rafael A. Espiritu
Asian Journal of Chemistry,
Vol. 34 No. 3 (2022): Vol 34 Issue 3, 2022
Abstract
Silver nanoclusters (AgNC) have gained considerable attention for their potential applications. In present work, crude protein extracts from the sea anemone Entacmaea quadricolor subjected to either heat or β-mercaptoethanol denaturation were prepared to investigate the effects of these processes in the formation of AgNC and on its preliminary bioactivity, measured using a hemolysis assay against human erythrocytes. Fourier-transform infrared spectrometry (FTIR) analysis of silver nanoclusters showed no significant alterations in protein secondary structure upon nanocluster formation for all samples. All samples, including the crude protein extracts, were found to be weakly hemolytic at the concentration range tested (0.5-1.0 mg protein/mL). These results imply that E. quadricolor crude protein denaturation prior to use with the reduction of Ag+ has no deleterious effects in functioning as capping agents for the prepared AgNC.
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- E. Pomerantseva, F. Bonaccorso, X. Feng, Y. Cui and Y. Gogotsi, Science, 366, eaan8285 (2019); https://doi.org/10.1126/science.aan8285
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F.D. Guerra, M.F. Attia, D.C. Whitehead and F. Alexis, Molecules, 23, 1760 (2018); https://doi.org/10.3390/molecules23071760
H. Su, Y. Wang, Y. Gu, L. Bowman, J. Zhao and M. Ding, J. Appl. Toxicol., 38, 3 (2018); https://doi.org/10.1002/jat.3476
S.K. Sonawane, A. Ahmad and S. Chinnathambi, ACS Omega, 4, 12833 (2019); https://doi.org/10.1021/acsomega.9b01411
H. Bagheri, A. Afkhami, H. Khoshsafar, A. Hajian and A. Shahriyari, Biosens. Bioelectron., 89, 829 (2017); https://doi.org/10.1016/j.bios.2016.10.003
D. Lu, C. Zhang, L. Fan, H. Wu, S. Shuang and C. Dong, Anal. Methods, 5, 5522 (2013); https://doi.org/10.1039/c3ay40901e
R.A. Espiritu and P.B.C.R. Rebustillo, Bionanoscience, 7, 501 (2017); https://doi.org/10.1007/s12668-017-0414-2
P. Singh, H. Singh, Y.J. Kim, R. Mathiyalagan, C. Wang and D.C. Yang, Enzyme Microb. Technol., 86, 75 (2016); https://doi.org/10.1016/j.enzmictec.2016.02.005
Y. Zhong, J. Zhu, Q. Wang, Y. He, Y. Ge and C. Song, Microchim. Acta, 182, 909 (2015); https://doi.org/10.1007/s00604-014-1407-2
H. Wei, Z. Wang, L. Yang, S. Tian, C. Hou and Y. Lu, Analyst, 135, 1406 (2010); https://doi.org/10.1039/c0an00046a
X.L. Guével, N. Daum and M. Schneider, Nanotechnology, 22, 275103 (2011); https://doi.org/10.1088/0957-4484/22/27/275103
F. Wen, Y. Dong, L. Feng, S. Wang, S. Zhang and X. Zhang, Anal. Chem., 83, 1193 (2011); https://doi.org/10.1021/ac1031447
I. Maliszewska, A. Juraszek and K. Bielska, J. Cluster Sci., 25, 989 (2014); https://doi.org/10.1007/s10876-013-0683-z
S.K. Das, C. Dickinson, F. Lafir, D.F. Brougham and E. Marsili, Green Chem., 14, 1322 (2012); https://doi.org/10.1039/c2gc16676c
H. Li, Y. Guo, L. Xiao and B. Chen, Analyst, 139, 285 (2014); https://doi.org/10.1039/C3AN01736B
H. Borbón, S. Váldes, J. Alvarado-Mesén, R. Soto and I. Vega, Asian Pac. J. Trop. Biomed., 6, 418 (2016); https://doi.org/10.1016/j.apjtb.2016.01.014
X. Le Guével, B. Hötzer, G. Jung, K. Hollemeyer, V. Trouillet and M. Schneider, J. Phys. Chem. C, 115, 10955 (2011); https://doi.org/10.1021/jp111820b
M.A. Huq, Int. J. Mol. Sci., 21, 1510 (2020); https://doi.org/10.3390/ijms21041510
D. Ballottin, S. Fulaz, M.L. Souza, P. Corio, A.G. Rodrigues, A.O. Souza, P.M. Gaspari, A.F. Gomes, F. Gozzo and L. Tasic, Nanoscale Res. Lett., 11, 313 (2016); https://doi.org/10.1186/s11671-016-1538-y
D. Usoltsev, V. Sitnikova, A. Kajava and M. Uspenskaya, Biomolecules, 9, 359 (2019); https://doi.org/10.3390/biom9080359
F. Raheman, S. Deshmukh, A. Ingle, A. Gade and M. Rai, Nano Biomed. Eng., 3, 174 (2011); https://doi.org/10.5101/nbe.v3i3.p174-178
K. Jyoti, M. Baunthiyal and A. Singh, J. Radiat. Res. Appl. Sci., 9, 217 (2016); https://doi.org/10.1016/j.jrras.2015.10.002
V. Banerjee and K.P. Das, Colloids Surf. B Biointerfaces, 111, 71 (2013); https://doi.org/10.1016/j.colsurfb.2013.04.052
N. Rojko, M. Dalla Serra, P. Maèek and G. Anderluh, Biochim. Biophys. Acta Biomembr., 1858, 446 (2016); https://doi.org/10.1016/j.bbamem.2015.09.007
N.M. El Salakawy, Ph.D. Thesis, Cytotoxic Activity of the Red Sea anemone Entacmaea quadricolor on Liver Cancer Cells, The American University, Cairo, Egypt (2018).
M. Ramezanpour, K. Burke da Silva and B.J. Sanderson, J. Venom. Anim. Toxins Incl. Trop. Dis., 18, 157 (2012); https://doi.org/10.1590/S1678-91992012000200005