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Vol. 37 No. 12 (2025): Vol 37 Issue 12, 2025

Copyright (c) 2025 SANDIP SEN SANDIP

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Green Synthesis and in vitro Antioxidant and Anticancer Activities of Copper Nanoparticles from Asparagus racemosus Chloroform Extract

https://doi.org/10.14233/ajchem.2025.34681
Jitendra Debata
Department of Pharmaceutical Chemistry, Bengal College of Pharmaceutical Technology, Dubrajpur, Birbhum-731123, India
https://orcid.org/0000-0003-3142-5362
Dhiraj Kumar
Department of Pharmaceutical Analysis, Institute of Technology and Management, GIDA, Gorakhpur-273212, India
https://orcid.org/0000-0001-6152-5837
Sujit Kumar Sahu
Department of Pharmaceutical Chemistry, Institute of Pharmacy & Technology, Salipur, Cuttack-754202, India
https://orcid.org/0009-0002-7792-6525
Tandrima Majumder
Department of Pharmaceutical Chemistry, College of Pharmacy, COER University, Roorkee, Haridwar-247667, India
https://orcid.org/0009-0006-4180-8972
Sandip Sen
Department of Pharmaceutical Chemistry, School of Pharmacy, Guru Nanak Institutions Technical Campus, Khanapur, Ibrahimpatnam-501506, India
https://orcid.org/0000-0002-6118-8567

Corresponding Author(s) : Sandip Sen

sandipsen2010@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 12 (2025): Vol 37 Issue 12, 2025

Abstract

Asparagus racemosus has a wide range of therapeutic activities due to different types of phytoconstituents like glycosides, alkaloids, steroids, tannins, lignins and other bioactive substances. To enhance the therapeutic efficacy of the highly non-polar chemicals present in the chloroform extract, the current study aimed to develop metallic nanoparticles using CuSO4·5H2O at 1 Mm copper sulphate. The extraction was carried out by Soxhlet apparatus using petroleum ether. The identification of the potential bioactive compound was done by GC-MS analysis. The optimization and characterization of the metallic nanoparticles were done by UV-Spectroscopy, particle size and zeta analysis, ESEM, TEM, FTIR and TGA. The in vitro antioxidant and anticancer activity were determined for the optimized formulation, moreover, in silico study was done by Swiss ADME, Moleinspiration and Oawasis data warrior software. The completion of the reaction was observed due to a change in absorption maxima. The particle size was determined to be 276.2 ± 8.1 nm, with a zeta potential of -50.09 ± 7.5 mV and a polydispersity index (PDI) of 0.29. The optimized nanoparticles exhibited a spherical morphology with a well-defined concentric bilayer membrane. No chemical incompatibilities were detected for the optimized formulation. Thermo-gravimetric and differential thermal analyses indicated favourable thermal stability, supporting a good shelf-life profile. The optimized nanoparticles exhibited good therapeutic efficacy due to improvement in the permeability of the chloroform extract and major constituent, steroidal compound pregn-5-ene-3,11-dione, 17,20:20,21-bis[methylenebis(oxy]]-, cyclic 3-(1,2-ethanediyl acetal).

Keywords

Asparagus racemosus Metallic nanoparticles Zeta potential Morphology Thermal analysis Antioxidant Anticancer activity
(1)
Debata, J.; Kumar, D.; Sahu, S. K.; Majumder, T.; Sen, S. Green Synthesis and in Vitro Antioxidant and Anticancer Activities of Copper Nanoparticles from Asparagus Racemosus Chloroform Extract. ajc 2025, 37, 3065-3072.
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