Determination of Creatinine in Human and Rat Urine by Capillary Zone Electrophoresis

Pingping Zhang; Xiaoli Wu; Xiaoyu Tian; Min Yao; Xin Lu; Kaoqi Lian; Lingling Jiang

doi:10.14233/ajchem.2015.17792

Issue

Vol. 27 No. 2 (2015): Vol 27 Issue 2

Issue Published : January 10, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Determination of Creatinine in Human and Rat Urine by Capillary Zone Electrophoresis

https://doi.org/10.14233/ajchem.2015.17792

Pingping Zhang

Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, P.R. China

Xiaoli Wu

Department of Pediatrics, the Second Hospital of Hebei Medical University, Shijiazhuang, P.R. China

Xiaoyu Tian

Department of Pediatrics, the Second Hospital of Hebei Medical University, Shijiazhuang, P.R. China

Min Yao

Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, P.R. China

Xin Lu

Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, P.R. China

Kaoqi Lian

Department of Sanitary Inspection, School of Public Health, Hebei Medical University, Shijiazhuang, P.R. China

Lingling Jiang

Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, P.R. China

Corresponding Author(s) : Kaoqi Lian

liankaoqi@126.com

Asian Journal of Chemistry, Vol. 27 No. 2 (2015): Vol 27 Issue 2
Article Published : January 10, 2015

Abstract

Creatinine is a very useful clinical marker of renal function and an internal standard to correct the excretion rate of other biological compounds in a urine sample. In this paper, a reliable, convenient and low-cost method for the determination of urinary creatinine by capillary zone electrophoresis was described. A typical calibration plot from 1 to 200 mg L^-1 (R² = 0.9978) with a detection limit (LOD) of 0.15 mg L^-1 (S/N = 3) is achieved. The relative standard deviation (RSD) was less than 4.2 % for peak area and 0.5 % for migration time, respectively. The proposed method was successfully applied to the determination of creatinine in both human urine and rat urine.

Keywords

Creatinine Capillary zone electrophoresis Human urine Rat urine

Zhang, P., Wu, X., Tian, X., Yao, M., Lu, X., Lian, K., & Jiang, L. (2015). Determination of Creatinine in Human and Rat Urine by Capillary Zone Electrophoresis. Asian Journal of Chemistry, 27(2), 705–708. https://doi.org/10.14233/ajchem.2015.17792

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References

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References

T. Smith-Palmer, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 781, 93 (2002); doi:10.1016/S1570-0232(02)00617-7.

L.A. Kaplan and A.J. Pesce, Clinical Chemistry: Theory, Analysis and Correlation, Mosby, New York, edn. 3 (1996).

H. Schiffl and S.M. Lang, Mol. Diagn. Ther., 16, 199 (2012); doi:10.1007/BF03262209.

H. Borsook and J.W. Dubnoff, J. Biol. Chem., 168, 493 (1974).

N.A. Devenport, J.C. Reynolds, V. Parkash, J. Cook, D.J. Weston and C.S. Creaser, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 879, 3797 (2011); doi:10.1016/j.jchromb.2011.10.016.

M. del Carmen Hurtado‐Sánchez, A. Espinosa‐Mansilla, M.I. Rodríguez‐Cáceres, E. Martín-Tornero and I. Durán-Merás, J. Sep. Sci., 35, 2575 (2012); doi:10.1002/jssc.201200255.

S.S. Waikar, V.S. Sabbisetti and J.V. Bonventre, Kidney Int., 78, 486 (2010); doi:10.1038/ki.2010.165.

C.W. Hu, C.J. Wang, L.W. Chang and M.R. Chao, Clin. Chem., 52, 1381 (2006); doi:10.1373/clinchem.2005.063735.

M.Z. Jaffe, Physiol. Chem., 10, 39 (1886).

S. Mohr, A. Willing, E. Radtke, K. Wolf and H. Hartmann, Kleintierpraxis, 52, 708 (2007).

V.F. Samanidou, A.S. Metaxa and I.N. Papadoyannis, J. Liq. Chromatogr. Rel. Technol., 25, 43 (2002); doi:10.1081/JLC-100108538.

Y. Jiang, X. Cheng, C. Wang and Y. Ma, Anal. Chem., 82, 9022 (2010); doi:10.1021/ac1019914.

S.M. Pedersen, C. Nebel, N.C. Nielsen, H.J. Andersen, J. Olsson, M. Simrén, L. Öhman, U. Svensson, H.C. Bertram and A. Malmendal, Eur. Food Res. Technol., 233, 1013 (2011); doi:10.1007/s00217-011-1599-1.

E. Poboży, A. Radomska, R. Koncki and S. Glab, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 789, 417 (2003); doi:10.1016/S1570-0232(03)00075-8.

A. Zinellu, C. Carru, M.F. Usai, S. Sotgia and L. Deiana, Electrophoresis, 25, 1096 (2004); doi:10.1002/elps.200305793.

A.C.O. Costa, J.L. da Costa, F.G. Tonin, M.F.M. Tavares and G.A. Micke, J. Chromatogr. A, 1171, 140 (2007); doi:10.1016/j.chroma.2007.09.029.

R. Paroni, I. Fermo, G. Cighetti, C.A. Ferrero, A. Carobene and F. Ceriotti, Electrophoresis, 25, 463 (2004); doi:10.1002/elps.200305687.

Issue

Vol. 27 No. 2 (2015): Vol 27 Issue 2

Determination of Creatinine in Human and Rat Urine by Capillary Zone Electrophoresis

Corresponding Author(s) : Kaoqi Lian

Abstract

Keywords

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