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Vol. 33 No. 4 (2021): Vol 33 Issue 4

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HPLC Determination of Formaldehyde in Flour Samples using 2,4,6-Trichlorophenyl Hydrazine as Derivatization Reagent

https://doi.org/10.14233/ajchem.2021.23120
Khaldun M. Al Azzam
Pharmacological and Diagnostic Research Center (PDRC), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan
Ahmad Makahleh
Department of Chemistry, Faculty of Science, University of Jordan, Amman 11942, Jordan
Bahruddin Saad
Fundamental and Applied Sciences Department, University Teknologi Petronas, 32610, Perak, Malaysia

Corresponding Author(s) : Khaldun M. Al Azzam

azzamkha@yahoo.com; k.azzam@ammanu.edu.jo

Asian Journal of Chemistry, Vol. 33 No. 4 (2021): Vol 33 Issue 4

Abstract

A new simple and sensitive high-performance liquid chromatography (HPLC) method for the determination of formaldehyde in flour samples has been developed. Formaldehyde was quantified after derivatization with a readily available reagent, 2,4,6-trichlorophenyl hydrazine (TCPH) under basic conditions. The formaldehyde-TCPH derivative was eluted with chromatographic mobile phase of 70:30 (v/v) acetonitrile:water at a flow rate of 1.0 mL min–1; wavelength, 222 nm; injection volume, 50 μL, using a C18 ODS Hypersil column (250 mm × 4.5 mm, 5 μm). The calibration curve was linear over the range of 0.001-10 μg mL-1 with R2 = 0.999. Recoveries at three different concentration levels (0.1, 1.0 and 5 μg mL-1) ranged from 92.0-101.7% with RSD less than of 2.2%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.3 and 1.0 ng mL-1, respectively. The developed method was used for the determination of formaldehyde in various flour-based samples.

Keywords

HPLC Flour Derivatization Formaldehyde 2,4,6-Trichlorophenyl hydrazine
M. Al Azzam, K., Makahleh, A., & Saad, B. (2021). HPLC Determination of Formaldehyde in Flour Samples using 2,4,6-Trichlorophenyl Hydrazine as Derivatization Reagent. Asian Journal of Chemistry, 33(4), 930–936. https://doi.org/10.14233/ajchem.2021.23120
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References
  1. X. Xu, R. Su, X. Zhao, Z. Liu, D. Li, X. Li, H. Zhang and Z. Wang, Talanta, 85, 2632 (2011); https://doi.org/10.1016/j.talanta.2011.08.037
  2. K.-H. Kim, S.A. Jahan and J.-T. Lee, J. Environ. Sci. Health, Part C,29, 277 (2011); https://doi.org/10.1080/10590501.2011.629972
  3. T. Wang, X. Gao, J. Tong and L. Chen, Food Chem., 131, 1577 (2012); https://doi.org/10.1016/j.foodchem.2011.10.021
  4. X.-Q. Zhao and Z-Q. Zhang, Talanta, 80, 242 (2009); https://doi.org/10.1016/j.talanta.2009.06.066
  5. Z. Li, H. Ma, H. Lu and G. Tao, Talanta, 74, 788 (2008); https://doi.org/10.1016/j.talanta.2007.07.011
  6. X. Weng, C.H. Chon, H. Jiang and D. Li, Food Chem., 114, 1079 (2009); https://doi.org/10.1016/j.foodchem.2008.10.027
  7. G. Burini and R. Coli, Anal. Chim. Acta, 511, 155 (2004); https://doi.org/10.1016/j.aca.2004.01.025
  8. A.A. Hill, R.J. Lipert, J.S. Fritz and M.D. Porter, Talanta, 77, 1405 (2009); https://doi.org/10.1016/j.talanta.2008.09.025
  9. L.S.G. Teixeira, E.S. Leão, A.F. Dantas, H.L.C. Pinheiro, A.C.S. Costa and J.B. de Andrade, Talanta, 64, 711 (2004); https://doi.org/10.1016/j.talanta.2004.03.047
  10. X. Cui, G. Fang, L. Jiang and S. Wang, Anal. Chim. Acta, 590, 253 (2007); https://doi.org/10.1016/j.aca.2007.03.042
  11. N.G. Yasri, H. Seddik and M.A. Mosallb, Arab. J. Chem., 8, 487 (2015); https://doi.org/10.1016/j.arabjc.2011.02.005
  12. L. Bolognesi, E.J. dos Santos and G. Abate, Chem. Pap., 69, 791 (2015); https://doi.org/10.1515/chempap-2015-0084
  13. A. Dar, U. Shafique, J. Anwar, Waheed-uz-Zaman and A. Naseer, J. Saudi Chem. Soc., 20, S352 (2016); https://doi.org/10.1016/j.jscs.2012.12.002
  14. H. Hayun, K. Harmita and T.B. Pramudita, Orient. J. Chem., 33, 1400 (2017); https://doi.org/10.13005/ojc/330341
  15. F. Bianchi, M. Careri, M. Musci and A. Mangia, Food Chem., 100, 1049 (2007); https://doi.org/10.1016/j.foodchem.2005.09.089
  16. H.S. Shin and H.H. Lim, Int. J. Food Sci. Technol., 47, 350 (2012); https://doi.org/10.1111/j.1365-2621.2011.02845.x
  17. F.S. De Oliveira, E.T. Sousa and J. Deandrade, Talanta, 73, 561 (2007); https://doi.org/10.1016/j.talanta.2007.04.027
  18. X.F. Yue, Y.N. Zhang and Z.Q. Zhang, Food Chem., 102, 90 (2007); https://doi.org/10.1016/j.foodchem.2006.05.005
  19. B. Horstkotte, E. Werner, S. Wiedemeier, O. Elsholz, V. Cerdá and R. Luttmann, Anal. Chim. Acta, 559, 248 (2006); https://doi.org/10.1016/j.aca.2005.11.085
  20. D. Zhang, J. Zhang, M. Li, W. Li, G. Aimaiti, G. Tuersun, J. Ye and Q. Chu, Food Chem., 129, 206 (2011); https://doi.org/10.1016/j.foodchem.2011.04.025
  21. J.F. Liu, J.F. Peng, Y.G. Chi and G.B. Jiang, Talanta, 65, 705 (2005); https://doi.org/10.1016/j.talanta.2004.07.037
  22. J. You, T. Yan, H. Zhao, Z. Sun, L. Xia, Y. Suo and Y. Li, Anal. Chim. Acta, 636, 95 (2009); https://doi.org/10.1016/j.aca.2009.01.036
  23. A.S. Sebaei, A. Gomaa, A.A. El-Zwahry and E.A. Emara, Int. J. Anal. Chem., 2018, 2757941 (2018); https://doi.org/10.1155/2018/2757941
  24. J.M. Storey, W.C. Andersen, A. Heise, S.B. Turnipseed, J. Lohne, T. Thomas and M. Madson, Food Addit. Contam. Part A, 32, 657 (2015); https://doi.org/10.1080/19440049.2015.1020530
  25. P. Wahed, M.A. Razzaq, S. Dharmapuri and M. Corrales, Food Chem., 202, 476 (2016); https://doi.org/10.1016/j.foodchem.2016.01.136
  26. S. Bhowmik, M. Begum, M.A. Hossain, M. Rahman and A.K.M.N. Alam, Egypt. J. Aquat. Res., 43, 245 (2017); https://doi.org/10.1016/j.ejar.2017.08.001
  27. P.A. Martos and J. Pawliszyn, Anal. Chem., 70, 2311 (1998); https://doi.org/10.1021/ac9711394
  28. J. Iglesias, J.M. Gallardo and I. Medina, Food Chem., 123, 771 (2010); https://doi.org/10.1016/j.foodchem.2010.05.025
  29. R.T. Rivero and V. Topiwala, J. Chromatogr. A, 1029, 217 (2004); https://doi.org/10.1016/j.chroma.2003.12.054
  30. Association of Official Analytical Chemists, Official Methods of Analysis of AOAC International, 2, AOAC International, Arlington ed. 16, p. 99 (1995).
  31. J. Peng, K. Fang, D. Xie, B. Ding, J.-Y. Yin, X. Cui, Y. Zhang and J. Liu, J. Chromatogr. A, 1209, 70 (2008); https://doi.org/10.1016/j.chroma.2008.09.028
  32. J.F. Lawrence and J.R. Iyengar, Int. J. Environ. Anal. Chem., 15, 47 (1983); https://doi.org/10.1080/03067318308071912
  33. M.T. Oliva-Teles, P. Paíga, C.M. Delerue-Matos and M.C.M. AlvimFerraz, Anal. Chim. Acta, 467, 97 (2002); https://doi.org/10.1016/S0003-2670(02)00130-7
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