In vitro Antitubercular Screening and in silico Study of Germacradienolide from Blainvillea latifolia

Varsha S. Honmore; Vidya K. Kalyankar; Arun D. Natu; Vijay M. Khedkar; Dhiman Sarkar; Supada R. Rojatkar

doi:10.14233/ajchem.2021.23537

Issue

Vol. 33 No. 12 (2021): Vol 33 Issue 12, 2021

Issue Published : December 6, 2021

This work is licensed under a Creative Commons Attribution 4.0 International License.

In vitro Antitubercular Screening and in silico Study of Germacradienolide from Blainvillea latifolia

https://doi.org/10.14233/ajchem.2021.23537

Varsha S. Honmore

Post Graduate and Research Center, Department of Chemistry, MES Abasaheb Garware College, Pune-411004, India

Vidya K. Kalyankar

Post Graduate and Research Center, Department of Chemistry, MES Abasaheb Garware College, Pune-411004, India

Arun D. Natu

Post Graduate and Research Center, Department of Chemistry, MES Abasaheb Garware College, Pune-411004, India

Vijay M. Khedkar

Department of Pharmaceutical Chemistry, School of Pharmacy, Vishwakarma University, Pune-411048, India

Dhiman Sarkar

Combichem-Bioresource Center, Organic Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411008, India

Supada R. Rojatkar

R&D Centre in Pharmaceutical Sciences and Applied Chemistry, Poona College of Pharmacy Campus, Bharati Vidyapeeth Deemed University, Erandwane, Pune-411038, India

Corresponding Author(s) : Varsha S. Honmore

vhonmore@gmail.com

Asian Journal of Chemistry, Vol. 33 No. 12 (2021): Vol 33 Issue 12, 2021
Article Published : December 6, 2021

Abstract

Bioassay-guided isolation from acetone extract of Blainvillea latifolia yielded one compound. The acetone extract, fractions and the compound 1 were investigated for antitubercular activity against Mycobacterium tuberculosis H37Ra. Compound 1 showed the activity with IC₅₀ and MIC values at 8.9 and >100 μg/mL. However, the acetone extract of Blainvillea latifolia was inactive against two Gram negative (E. coli, P. flurescence) and two Gram-positive (S. aureus, B. subtilis) bacterial strains. Hence, it was concluded that the extract and the compound 1 are specifically active against MTB and not against bacterial strains. Molecular docking study was performed against crucial mycobacterial target MtInhA to gain an insight into the binding mode and the thermodynamic interactions governing the binding affinity of this molecule.

Keywords

Blainvillea latifolia Asteraceae Germacradienolide Antitubercular activity Molecular docking

S. Honmore, V., K. Kalyankar, V., D. Natu, A., M. Khedkar, V., Sarkar, D., & R. Rojatkar, S. (2021). In vitro Antitubercular Screening and in silico Study of Germacradienolide from Blainvillea latifolia. Asian Journal of Chemistry, 33(12), 3129–3133. https://doi.org/10.14233/ajchem.2021.23537

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References

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E. Yamuna, R.A. Kumar, M. Zeller and K.J. Rajendra Prasad, Eur. J. Med. Chem., 47, 228 (2012); https://doi.org/10.1016/j.ejmech.2011.10.046

V. Krishna, V. Sharma and P. Singh, J. Indian Chem. Soc., 82, 214 (2005).

V. Krishna, P.K. Gupta, S. Jain and P. Singh, J. Indian Chem. Soc., 78, 779 (2001).

P. Singh, R. Jain and V. Krishna, Nat. Prod. Sci., 4, 42 (1998).

D.D. Sawaikar, S.R. Rojatkar and B.A. Nagasampagi, Phytochemistry, 46, 375 (1997); https://doi.org/10.1016/S0031-9422(97)00233-1

D.D. Sawaikar, S.R. Rojatkar and B.A. Nagasampagi, Phytochemistry, 36, 399 (1994); https://doi.org/10.1016/S0031-9422(00)97083-3

P. Singh, M. Bhala, R. Jain and J. Jakupovic, Phytochemistry, 27, 609 (1988); https://doi.org/10.1016/0031-9422(88)83152-2

J.S. Sohoni, S.R. Rojatkar, M.M. Kulkarni, N.N. Dhaneshwar, S.S. Tavale, T.N. Gururow and B.A. Nagasampagi, J. Chem. Soc., Perkin Trans. 1, 157 (1988); https://doi.org/10.1039/P19880000157

N. Adityachaudhury and A. Chowdhury, Phytochemistry, 11, 3544 (1972); https://doi.org/10.1016/S0031-9422(00)89860-X

U. Singh, S. Akhtar, A. Mishra and D. Sarkar, J. Microbiol. Methods, 84, 202 (2011); https://doi.org/10.1016/j.mimet.2010.11.013

S. Sarkar and D. Sarkar, J. Biomol. Screen., 17, 966 (2012); https://doi.org/10.1177/1087057112445485

J.P. Dzoyem, S.K. Guru, C.A. Pieme, V. Kuete, A. Sharma, I.A. Khan, A.K. Saxena and R.A. Vishwakarma, BMC Complement. Altern. Med., 13, 78 (2013); https://doi.org/10.1186/1472-6882-13-78

C.L. Cantrell, I.S. Nuñez, J. Castañeda-Acosta, M. Foroozesh, F.R. Fronczek, N.H. Fischer and S.G. Franzblau, J. Nat. Prod., 61, 1181 (1998); https://doi.org/10.1021/np970333i

S. Kang, R.Y. Kim, M.J. Seo, S. Lee, Y.M. Kim, M. Seo, J.J. Seo, Y. Ko, I. Choi, J. Jang, J. Nam, S. Park, H. Kang, H.J. Kim, J. Kim, S. Ahn, K. Pethe, K. Nam, Z. No and J. Kim, J. Med. Chem., 57, 5293 (2014); https://doi.org/10.1021/jm5003606

R.J. Friesner, R.B. Murphy, M.P. Repasky, L.L. Frye, J.R. Greenwood, T.A. Halgren, P.C. Sanschagrin and D.T. Mainz, Med. Chem., 49, 6177 (2006); https://doi.org/10.1021/jm051256o

T.A. Halgren, R.B. Murphy, R.A. Friesner, H.S. Beard, L.L. Frye, W.T. Pollard and J.L. Banks, J. Med. Chem., 47, 1750 (2004); https://doi.org/10.1021/jm030644s

R.A. Friesner, J.L. Banks, R.B. Murphy, T.A. Halgren, J.J. Klicic, D.T. Mainz, M.P. Repasky, E.H. Knoll, M. Shelley, J.K. Perry, D.E. Shaw, P. Francis and P.S. Shenkin, J. Med. Chem., 47, 1739 (2004); https://doi.org/10.1021/jm0306430

Issue

Vol. 33 No. 12 (2021): Vol 33 Issue 12, 2021

In vitro Antitubercular Screening and in silico Study of Germacradienolide from Blainvillea latifolia

Corresponding Author(s) : Varsha S. Honmore

Abstract

Keywords

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