Sequestration of Stigmasterol and &beta;-Sitosterol from Ethanolic Extract of Kam Sabut (Croton caudatus Geiseler) Leaves

Longjam Shantabi; Ganesh Chandra Jagetia; Sharubam Victoria Devi; H. Lalhenmawia; Raghuvir H. Gaonkar

doi:10.14233/ajchem.2021.23426

Issue

Vol. 33 No. 11 (2021): Vol 33 Issue 11, 2021

Issue Published : October 20, 2021

This work is licensed under a Creative Commons Attribution 4.0 International License.

Sequestration of Stigmasterol and β-Sitosterol from Ethanolic Extract of Kam Sabut (Croton caudatus Geiseler) Leaves

https://doi.org/10.14233/ajchem.2021.23426

Longjam Shantabi

College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Selesih, Aizawl-796014, India

Ganesh Chandra Jagetia

Department of Zoology, Mizoram University, Tanhril, Aizawl-796012, India

Sharubam Victoria Devi

Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk, Aizawl-796017, India

H. Lalhenmawia

Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk, Aizawl-796017, India

Raghuvir H. Gaonkar

Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata-700032, India

Corresponding Author(s) : Longjam Shantabi

Asian Journal of Chemistry, Vol. 33 No. 11 (2021): Vol 33 Issue 11, 2021
Article Published : October 20, 2021

Abstract

Croton caudatus (Kam sabut) has been traditionally used to treat cancer in northeastern India and earlier studies reported the anticancer activity of ethanolic extract of C. caudatus leaves in HeLa cells in vitro and Swiss albino mice transplanted with Dalton’s ascites lymphoma. Therefore, isolation of stigmasterol and β-sitosterol from the ethanolic extract of C. caudatus Geiseler leaves was carried out. Stigmasterol and β-sitosterol were isolated from the ethanolic extract of dried leaves of C. caudatus by column chromatography. The fractions were purified further on Sephadex^® LH-20 column chromatography and reverse-phase HPLC. The isolated phytosterols were characterized and quantified by IR, ¹H & ¹³C NMR, mass spectroscopy and HPLC. This has led to the separation of 100 mg stigmasterol and 80 mg β-sitosterol. Stigmasterol and β-sitosterol have been isolated from the ethanolic extract of C. caudatus leaves for the first time.

Keywords

Croton caudatus Stigmasterol Column chromatography β-sitosterol

Shantabi, L., Chandra Jagetia, G., Victoria Devi, S., Lalhenmawia, H., & H. Gaonkar, R. (2021). Sequestration of Stigmasterol and β-Sitosterol from Ethanolic Extract of Kam Sabut (Croton caudatus Geiseler) Leaves. Asian Journal of Chemistry, 33(11), 2851–2856. https://doi.org/10.14233/ajchem.2021.23426

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