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A New Concept for Testing Chromatographic Peak Purity: Application to Peak Purity Determination of Main Ingredients in Tea Extracts
Corresponding Author(s) : Marwa S. Moneeb
Asian Journal of Chemistry,
Vol. 27 No. 10 (2015): Vol 27 Issue 10
Abstract
A new concept was applied to test the spectral purity of chromatographic peaks. The proposed concept is based on the application of the orthogonal functions (P1 and P2) to the absorption spectra recorded at five different time intervals throughout the eluted chromatographic peak. Ratios of strong peaks of the p1- and p2- convoluted absorption spectra were computed for the investigated samples and for standard solutions of the corresponding reference materials. Such ratios are considered spectrophotometric characteristics for each compound, independent of concentration. For pure peaks, the calculated orthogonal ratios at different time intervals were similar to those of spectrum apex with RSD values less than 1 %. This new concept was applied to determine the peak purity of the main alkaloids in tea extracts which contain different interfering structurally related substances. The proposed concept could be considered a universal technique for peak purity assessment in any quality control laboratory.
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- M. Stahl, Peak Purity Analysis in HPLC and CE Using Diode-Array Technology, Waldbronn, Germany (2003).
- S. Kodama, A. Yamamoto, Y. Saito, N. Takayanagi and A. Matsunaga, Bunseki Kagaku, 45, 259 (1996); doi:10.2116/bunsekikagaku.45.259.
- J. Ning, G. Guoqiang, C. Yuying, H. Yuzhu, D. Xiangdong and Y. Juan, J. China Pharm. Univ., 24, 112 (1993).
- H. Fabre, A. Le Bris and M.D. Blanchin, J. Chromatogr. A, 697, 81 (1995); doi:10.1016/0021-9673(94)00979-J.
- I.I. Hewala, M.M. Bedair and S.M. Shousha, Talanta, 88, 623 (2012); doi:10.1016/j.talanta.2011.11.048.
- A.F. Fell, H.P. Scott, R. Gill and A.C. Moffat, J. Chromatogr. A, 282, 123 (1983); doi:10.1016/S0021-9673(00)91597-8.
- I. Hewala, H. El-Fatatry, E. Emam and M. Mabrouk, J. AOAC Int., 94, 1447 (2011); doi:10.5740/jaoacint.10-129.
- I. Hewala, H. El-Fatatre, E. Emam and M. Mubrouk, Talanta, 82, 184 (2010); doi:10.1016/j.talanta.2010.04.017.
- A.L. Glenn, J. Pharm. Pharmacol., 15(S1), 123T (1963); doi:10.1111/j.2042-7158.1963.tb11198.x.
- W.E. Milne, Expansion of an Absorption Curve in Terms of Orthogonal Polynomials; Numerical Calculus, Princeton University Press, Princeton, USA, edn 1 (1949).
- A.M. Wahbi, Ph.D. Thesis, Application of Orthogonal Functions to Spectrophotometric Analysis. University of London (1967).
- I.U. Agwu and A.L. Glenn, J. Pharm. Pharmacol., 19(Suppl), 76S (1967).
- A.M. Wahbi, A.A. Gazy, O. Abdel-Razak, H. Mahgoub and M.S. Moneeb, Saudi Pharm. J., 11, 192 (2003).
- A.M. Wahbi, M.S. Moneeb, I.I. Hewala and M.F. Bahnasy, Bull. Fac. Pharm. Cairo Univ., 47, 192 (2009).
- G. Essam, M.Sc. Thesis, Application of Orthogonal Functions to Verify Peak Purity; Determination of Certain Mixtures of Pharmaceutical Interest, University of Alexandria, Alexandria, Egypt (2014).
- L.L. Martinez, P.L. De-Alba, R.G. Campos and L.M. Rodriguez, Anal. Chim. Acta, 493, 83 (2003); doi:10.1016/S0003-2670(03)00862-6.
- The British Pharmacopeia, Her Majesty Stationary Office, London, CD ROM electronic version (2013).
- A.M. Wahbi, F.A. El-Yazbi, M.H. Barary and S. Sabry, Alex. J. Pharm. Sci., 5, 220 (1991).
References
M. Stahl, Peak Purity Analysis in HPLC and CE Using Diode-Array Technology, Waldbronn, Germany (2003).
S. Kodama, A. Yamamoto, Y. Saito, N. Takayanagi and A. Matsunaga, Bunseki Kagaku, 45, 259 (1996); doi:10.2116/bunsekikagaku.45.259.
J. Ning, G. Guoqiang, C. Yuying, H. Yuzhu, D. Xiangdong and Y. Juan, J. China Pharm. Univ., 24, 112 (1993).
H. Fabre, A. Le Bris and M.D. Blanchin, J. Chromatogr. A, 697, 81 (1995); doi:10.1016/0021-9673(94)00979-J.
I.I. Hewala, M.M. Bedair and S.M. Shousha, Talanta, 88, 623 (2012); doi:10.1016/j.talanta.2011.11.048.
A.F. Fell, H.P. Scott, R. Gill and A.C. Moffat, J. Chromatogr. A, 282, 123 (1983); doi:10.1016/S0021-9673(00)91597-8.
I. Hewala, H. El-Fatatry, E. Emam and M. Mabrouk, J. AOAC Int., 94, 1447 (2011); doi:10.5740/jaoacint.10-129.
I. Hewala, H. El-Fatatre, E. Emam and M. Mubrouk, Talanta, 82, 184 (2010); doi:10.1016/j.talanta.2010.04.017.
A.L. Glenn, J. Pharm. Pharmacol., 15(S1), 123T (1963); doi:10.1111/j.2042-7158.1963.tb11198.x.
W.E. Milne, Expansion of an Absorption Curve in Terms of Orthogonal Polynomials; Numerical Calculus, Princeton University Press, Princeton, USA, edn 1 (1949).
A.M. Wahbi, Ph.D. Thesis, Application of Orthogonal Functions to Spectrophotometric Analysis. University of London (1967).
I.U. Agwu and A.L. Glenn, J. Pharm. Pharmacol., 19(Suppl), 76S (1967).
A.M. Wahbi, A.A. Gazy, O. Abdel-Razak, H. Mahgoub and M.S. Moneeb, Saudi Pharm. J., 11, 192 (2003).
A.M. Wahbi, M.S. Moneeb, I.I. Hewala and M.F. Bahnasy, Bull. Fac. Pharm. Cairo Univ., 47, 192 (2009).
G. Essam, M.Sc. Thesis, Application of Orthogonal Functions to Verify Peak Purity; Determination of Certain Mixtures of Pharmaceutical Interest, University of Alexandria, Alexandria, Egypt (2014).
L.L. Martinez, P.L. De-Alba, R.G. Campos and L.M. Rodriguez, Anal. Chim. Acta, 493, 83 (2003); doi:10.1016/S0003-2670(03)00862-6.
The British Pharmacopeia, Her Majesty Stationary Office, London, CD ROM electronic version (2013).
A.M. Wahbi, F.A. El-Yazbi, M.H. Barary and S. Sabry, Alex. J. Pharm. Sci., 5, 220 (1991).