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Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Copyright (c) 2024 R. Ratna Manjula, M. Thirumal

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Phytochemical Screening, Isolation and Characterization of Bioactive Compounds from Ethanol Extract of Memecylon lushingtonii Gamble

https://doi.org/10.14233/ajchem.2024.32771
R. Ratna Manjula
Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu-603203, India
https://orcid.org/0000-0002-0901-3460
M. Thirumal
Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu-603203, India
https://orcid.org/0000-0002-9879-1140

Corresponding Author(s) : M. Thirumal

thirumam@srmist.edu.in

Asian Journal of Chemistry, Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Abstract

The primary goal of this study was to isolate and characterize the bioactive compounds present in the M. lushingtonii Gamble leaves using ethanol as solvent for the extraction using Soxhlet apparatus for 8 h. Based on the chemical analysis, column chromatography using different solvents depending on polarity further isolated the components. Isolation of the extract was carried out using toluene and ethyl acetate solvents with varying concentrations and showed two single spots by TLC at the rentation time (Rt) of 0.66 and 0.72 for the solvent’s toluene and ethyl acetate (8:12 and 2:18), respectively. Various spectroscopy techniques (UV, NMR, IR, and MASS) were employed for the characterisation of the compounds and elucidation of their structures. The results confirmed the presence of alkaloids, phenolics, flavonoids, tannins, sterols and terpenoids. The spectroscopic analysis revealed the compounds were quercetin (Rf: 0.66) and luteolin (Rf: 0.72) which were both flavonoid group and contains five and four –OH groups, respectively in their basic structure (C6-C3-C6). The results demonstrated that the leaves of M. lushingtonii Gamble exhibited a higher concentration of polyphenolic chemicals, which contribute to the diverse therapeutic properties.

Keywords

M. lushingtonii Gamble leaves Bioactive compounds Phenolics Flavonoids
Manjula, R. R., & Thirumal, M. (2024). Phytochemical Screening, Isolation and Characterization of Bioactive Compounds from Ethanol Extract of Memecylon lushingtonii Gamble. Asian Journal of Chemistry, 36(12), 2967–2971. https://doi.org/10.14233/ajchem.2024.32771
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