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Vol. 32 No. 9 (2020): Vol 32 Issue 9, 2020

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in vitro Screening of Sesamum indicum Seeds for Antioxidant, Phytochemical and Biological Properties

https://doi.org/10.14233/ajchem.2020.22775
Sheraz Khan
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan; Pharmacy, Al-Shefa Medical Complex (SMC), Tabuk city, Kingdom of Saudi Arabia
Saniya Hashim Khan
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan ; Allied Health Science Department, Rehman Medical Institute, Peshawar, Pakistan
Inam Ullah Khan
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan
Wali Inam
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan ; Faculty of Medicine, University of Turku, Turku, Finland
Muhammad Asim
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan
Narmeen Hashim
Department of Medical Research, Rehman Medical College, Khyber Pukhtunkhwa, Peshawar, Pakistan

Corresponding Author(s) : Sheraz Khan

sherazkhan0331@gmail.com

Asian Journal of Chemistry, Vol. 32 No. 9 (2020): Vol 32 Issue 9, 2020

Abstract

Herbal medicines has been the most cost-effective and valuable medical practice to cure diseases and emphasize modern health care treatment. The present research was conducted to assess the biological activities of 10 fractions obtained from methanolic extract which was derived from dried seeds of Sesamum indicum. Antioxidant activity was assessed using DPPH radical scavenging, total antioxidant capacity and total reducing power assays. Highest free radical scavenging activity (80.3 ± 1.36%), total antioxidant capacity (104.7 ± 4.04 μg AAE/mg) and ferric reducing power activity (238.76 ± 1.23 μg AAE/mg) was shown by fraction SE. Fraction SE showed the highest phenolic contents (63.72 ± 1.5 μg GAE/mg) while fraction SG sample showed highest flavonoid contents (54.62 ± 2.61 μg QE QE/mg). Antibacterial activity was performed against four selected bacterial strains including Staphylococcus aureus, Escherichia coli, Enterobacter aerogens and Bacillus subtilis. Highest inhibition was shown by fraction SD (11 ± 1.04 mm) against Staphylococcus aureus, fraction SH against Bacillus subtilis (11 ± 1.06 mm) and fraction SB against Escherichia coli. All fractions were found inactive against the selected fungal strains. While performing antileishmanial activity, fraction SC showed highest percent mortality (78%) of Leishmania tropica. In brine shrimp lethality bioassay, fraction SG showed significant LD50 value (23.48 μg/mL).

Keywords

Sesamum indicum Sesamum indicum Antioxidant assay Antioxidant assay Antimicrobial assay Antimicrobial assay Antileishmanial assay Antileishmanial assay Cytotoxic assay Cytotoxic assay
Khan, S., Hashim Khan, S., Ullah Khan, I., Inam, W., Asim, M., & Hashim, N. (2020). in vitro Screening of Sesamum indicum Seeds for Antioxidant, Phytochemical and Biological Properties. Asian Journal of Chemistry, 32(9), 2262–2266. https://doi.org/10.14233/ajchem.2020.22775
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