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Separation of Fatty Acid Ethanolamides using High Performance Liquid Chromatography
Corresponding Author(s) : Erin Ryantin Gunawan
Asian Journal of Chemistry,
Vol. 32 No. 12 (2020): Vol 32 Issue 12, 2020
Abstract
This study aimed to separate fatty acid ethanolamides (FAE) through an enzymatic reaction of ethanolamine and the Terminalia catappa (Local name: Ketapang) seed oil by using reverse-phase high-performance liquid chromatography (RP-HPLC). A 4.6 mm × 150 mm, 5 μm SunFire C18 column was used. Separation optimization was performed by analyzing different variables, which includes mobile phase composition (acetonitrile/water), flow rate and sample concentration. The separation results showed that the retention time for all components was 4.5; 2.4; 12.9; and 6.3 min for fatty acid ethanolamides: oleoylethanolamide, linoleoylethanolamide, stearyl diethanolamide and palmitoylethanolamide, respectively. Inter-peak resolution of >1.5 indicated that each component was accurately separated.
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- M.M.A. El-Sukkary, N.O. Shaker, D.A. Ismail, S.M. Ahmed and A.I. Awad, Egyp. J. Petrol., 21, 11 (2012); https://doi.org/10.1016/j.ejpe.2012.02.002
- A. Adewuyi, Arab. J. Chem., 12, 1545 (2019); https://doi.org/10.1016/j.arabjc.2014.10.021
- D. Kumar, S.M. Kim and A. Ali, New J. Chem., 39, 7097 (2015); https://doi.org/10.1039/C5NJ00388A
- X. Wang, Y. Chen, Q. Jin, J. Huang and X. Wang, J. Oleo Sci., 62, 427 (2013); https://doi.org/10.5650/jos.62.427
- E.R. Gunawan, D. Suhendra, I. Hidayat and L. Kurniawati, J. Oleo Sci., 67, 949 (2018); https://doi.org/10.5650/jos.ess18042
- X. Wang, Z. Han, Y. Chen, Q. Jin and X. Wang, J. Am. Oil Chem. Soc.,93, 125 (2016);https://doi.org/10.1007/s11746-015-2749-6
- J. Chapman, A.E. Ismail and C.Z. Dinu, Catalyst, 8, 238 (2018); https://doi.org/10.3390/catal8060238
- E.R. Gunawan, D. Suhendra, Trisnasari and L. Kurniawati, J. Phys. Conf. Ser., 1095, 012014 (2018); https://doi.org/10.1088/1742-6596/1095/1/012014
- S. Datta, L.R. Christena and Y.R.S. Rajaram, 3 Biotech., 3, 1 (2013); https://doi.org/10.1007/s13205-012-0071-7
- A.V. Anand, N. Divya and P.P. Kotti, Pharmacogn. Rev., 9, 93 (2015); https://doi.org/10.4103/0973-7847.162103
- F. Sari, B.H. Susanto and S. Bimo, IOP Conf. Ser.: Earth Environ. Sci., 105, 012035 (2018); https://doi.org/10.1088/1755-1315/105/1/01203
- A.A. Bele and A. Khale, Int. J. Pharm. Sci., 2, 256 (2011).
- M. Chin, A. Quinlan and P. Khandelwal, The Column, 13, 8 (2017).
- W. Letter, Consultant & HPLC Expert, Company: Chiralizer Services, LLC, Hillsborough, NC USA (2015).
- C.E. Calvet, C.O. Bolsico, J.B. Baeza and M.C. Garcia-Alavrez-Coque, Chromatography, 1, 194 (2014); https://doi.org/10.3390/Chromatography1040194
- J.W. Dolan, LC Resources Inc., Walnut Creek, California, USA (2014).
- Y. Guo, N. Bhalodia, B. Fattal and I. Serris, Separations, 6, 1 (2019); https://doi.org/10.3390/separations6020019
References
M.M.A. El-Sukkary, N.O. Shaker, D.A. Ismail, S.M. Ahmed and A.I. Awad, Egyp. J. Petrol., 21, 11 (2012); https://doi.org/10.1016/j.ejpe.2012.02.002
A. Adewuyi, Arab. J. Chem., 12, 1545 (2019); https://doi.org/10.1016/j.arabjc.2014.10.021
D. Kumar, S.M. Kim and A. Ali, New J. Chem., 39, 7097 (2015); https://doi.org/10.1039/C5NJ00388A
X. Wang, Y. Chen, Q. Jin, J. Huang and X. Wang, J. Oleo Sci., 62, 427 (2013); https://doi.org/10.5650/jos.62.427
E.R. Gunawan, D. Suhendra, I. Hidayat and L. Kurniawati, J. Oleo Sci., 67, 949 (2018); https://doi.org/10.5650/jos.ess18042
X. Wang, Z. Han, Y. Chen, Q. Jin and X. Wang, J. Am. Oil Chem. Soc.,93, 125 (2016);https://doi.org/10.1007/s11746-015-2749-6
J. Chapman, A.E. Ismail and C.Z. Dinu, Catalyst, 8, 238 (2018); https://doi.org/10.3390/catal8060238
E.R. Gunawan, D. Suhendra, Trisnasari and L. Kurniawati, J. Phys. Conf. Ser., 1095, 012014 (2018); https://doi.org/10.1088/1742-6596/1095/1/012014
S. Datta, L.R. Christena and Y.R.S. Rajaram, 3 Biotech., 3, 1 (2013); https://doi.org/10.1007/s13205-012-0071-7
A.V. Anand, N. Divya and P.P. Kotti, Pharmacogn. Rev., 9, 93 (2015); https://doi.org/10.4103/0973-7847.162103
F. Sari, B.H. Susanto and S. Bimo, IOP Conf. Ser.: Earth Environ. Sci., 105, 012035 (2018); https://doi.org/10.1088/1755-1315/105/1/01203
A.A. Bele and A. Khale, Int. J. Pharm. Sci., 2, 256 (2011).
M. Chin, A. Quinlan and P. Khandelwal, The Column, 13, 8 (2017).
W. Letter, Consultant & HPLC Expert, Company: Chiralizer Services, LLC, Hillsborough, NC USA (2015).
C.E. Calvet, C.O. Bolsico, J.B. Baeza and M.C. Garcia-Alavrez-Coque, Chromatography, 1, 194 (2014); https://doi.org/10.3390/Chromatography1040194
J.W. Dolan, LC Resources Inc., Walnut Creek, California, USA (2014).
Y. Guo, N. Bhalodia, B. Fattal and I. Serris, Separations, 6, 1 (2019); https://doi.org/10.3390/separations6020019