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Development and Validation of UV Spectrophotometric and RP-HPLC Methods for the Estimation of Gallic Acid in Herbal Formulation of Amalaki
Corresponding Author(s) : Mithun Rudrapal
Asian Journal of Chemistry,
Vol. 32 No. 10 (2020): Vol 32 Issue 10
Abstract
In this study, two analytical methods viz. UV spectrophotometry and RP-HPLC were developed for the evaluation of Amalaki in the marketed herbal formulation. The amount of gallic acid estimated in the marketed formulation complies with the standard (not less than 1% w/w of gallic acid) specified in the official monograph of Amalaki. The developed methods showed good linearity, accuracy, precision, ruggedness, robustness, specificity, LOD and LOQ. Results of validation studies were found satisfactory with % RSD values of less than 2% indicating good specificity, validity and reliability of the developed methods. Both analytical methods are claimed to be simple, accurate and precise. Present methods can, therefore, be applied widely for the routine analysis of marketed formulations or any crude traditional preparations of Amalaki based on the quantitative determination of gallic acid at a reasonable cost with simple analytical set up.
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- M.R. Hasan, M.N. Islam and M.R. Islam, Int. Curr. Pharm. J., 5, 14 (2016).
- A. Biswas, A. Chatterjee and S. Bahdhopadhyay, J. Pharm. Innov., 2, 72 (2012).
- P. Das, T. Panneerselvam, P.P. Prabhu and J. Reddy, Int. J. Curr. Res., 9, 61417 (2017).
- S.V. Fulmali and P.A. Tatke, Eur. J. Pharm. Med. Res., 4, 452 (2017).
- P.S. Warule, V.P. Patel and S.A. Gosavi, Int. J. Pharm. Drug Anal., 5, 193 (2017).
- V. Bansal, A. Sharma, C. Ghanshyam and M.L. Singla, Anal. Methods, 6, 410 (2014); https://doi.org/10.1039/C3AY41375F
- L. Sawant, N. Pandita and B. Prabhakar, J. Pharm. Bioallied Sci., 2, 105 (2010); https://doi.org/10.4103/0975-7406.67012
- L. Sawant, B. Prabhakar and N. Pandita, J. Anal. Bioanal. Tech., 1, 2 (2010); https://doi.org/10.4172/2155-9872.1000111
- S.J. Patil and V.R. Salunkhe, Int. J. Res. Ayurveda Pharm., 3, 267 (2012).
- K. Kardani, N. Gurav, B. Solanki, P. Patel and B. Patel, J. Appl. Pharm. Sci., 3, 37 (2013).
- S. Joshi, V.M. Jadav and V.J. Kadam, World J. Pharm. Sci., 7, 1693 (2018).
- K.H. Kachhadiya, J.S. Shah and D. Maheshwari, Int. J. Pharm. Res. Biosci., 3, 548 (2014).
- J.M. Green, Anal. Chem., 68, 305A (1996); https://doi.org/10.1021/ac961912f
- International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Methodology, ICH-Q2B, Geneva (1996).
- M. Rudrapal, M. Oduri, N. Samidala, B.V.V.S. Kiran, J. Junejo, K. Singh, T. Chakraborty and M. Debnath, Orient. J. Chem., 31, 921 (2015); https://doi.org/10.13005/ojc/310236
- M. Rudrapal, B.V.V.S. Surya Kiran, N. Sridhar and M. Raghavendra, Asian J. Chem., 28, 157 (2016); https://doi.org/10.14233/ajchem.2016.19290
- W. Wegscheider, ed.: H. Gunzler, Validation of Analytical Methods, In: Accreditation and Quality Assurance in Analytical Chemistry, Springer: Berlin (1996).
- M.G. Patel, V.R. Patel and R.K. Patel, Int. J. ChemTech Res., 2, 1486 (2010).
- N.P. Pawar and V.R. Salunkhe, Int. J. Pharm. Tech. Res., 5, 724 (2013).
- P.J. Purohit, P.P. Kapupara and K.V. Shah, Res. J. Pharm. Tech., 7, 749 (2014).
References
M.R. Hasan, M.N. Islam and M.R. Islam, Int. Curr. Pharm. J., 5, 14 (2016).
A. Biswas, A. Chatterjee and S. Bahdhopadhyay, J. Pharm. Innov., 2, 72 (2012).
P. Das, T. Panneerselvam, P.P. Prabhu and J. Reddy, Int. J. Curr. Res., 9, 61417 (2017).
S.V. Fulmali and P.A. Tatke, Eur. J. Pharm. Med. Res., 4, 452 (2017).
P.S. Warule, V.P. Patel and S.A. Gosavi, Int. J. Pharm. Drug Anal., 5, 193 (2017).
V. Bansal, A. Sharma, C. Ghanshyam and M.L. Singla, Anal. Methods, 6, 410 (2014); https://doi.org/10.1039/C3AY41375F
L. Sawant, N. Pandita and B. Prabhakar, J. Pharm. Bioallied Sci., 2, 105 (2010); https://doi.org/10.4103/0975-7406.67012
L. Sawant, B. Prabhakar and N. Pandita, J. Anal. Bioanal. Tech., 1, 2 (2010); https://doi.org/10.4172/2155-9872.1000111
S.J. Patil and V.R. Salunkhe, Int. J. Res. Ayurveda Pharm., 3, 267 (2012).
K. Kardani, N. Gurav, B. Solanki, P. Patel and B. Patel, J. Appl. Pharm. Sci., 3, 37 (2013).
S. Joshi, V.M. Jadav and V.J. Kadam, World J. Pharm. Sci., 7, 1693 (2018).
K.H. Kachhadiya, J.S. Shah and D. Maheshwari, Int. J. Pharm. Res. Biosci., 3, 548 (2014).
J.M. Green, Anal. Chem., 68, 305A (1996); https://doi.org/10.1021/ac961912f
International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Methodology, ICH-Q2B, Geneva (1996).
M. Rudrapal, M. Oduri, N. Samidala, B.V.V.S. Kiran, J. Junejo, K. Singh, T. Chakraborty and M. Debnath, Orient. J. Chem., 31, 921 (2015); https://doi.org/10.13005/ojc/310236
M. Rudrapal, B.V.V.S. Surya Kiran, N. Sridhar and M. Raghavendra, Asian J. Chem., 28, 157 (2016); https://doi.org/10.14233/ajchem.2016.19290
W. Wegscheider, ed.: H. Gunzler, Validation of Analytical Methods, In: Accreditation and Quality Assurance in Analytical Chemistry, Springer: Berlin (1996).
M.G. Patel, V.R. Patel and R.K. Patel, Int. J. ChemTech Res., 2, 1486 (2010).
N.P. Pawar and V.R. Salunkhe, Int. J. Pharm. Tech. Res., 5, 724 (2013).
P.J. Purohit, P.P. Kapupara and K.V. Shah, Res. J. Pharm. Tech., 7, 749 (2014).