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Vol. 37 No. 1 (2025): Vol 37 Issue 1, 2025

Copyright (c) 2024 Aswin Gowri Prasad, Yagniyasree Manogaran, Pasiyappazham Ramasamy

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Synthesis, Characterization and Antioxidant Activities of Bioactive Polymer from Internal Bone of Sepia aculeata

https://doi.org/10.14233/ajchem.2025.32838
Aswin Gowri Prasad
Department of Physiology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India
Yagniyasree Manogaran
Polymer Research Laboratory, Centre for Marine and Aquatic Research (CMAR), Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-602105, India; Department of Prosthodontics and Implantology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India
Pasiyappazham Ramasamy
Polymer Research Laboratory, Centre for Marine and Aquatic Research (CMAR), Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-602105, India; Department of Prosthodontics and Implantology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India
https://orcid.org/0000-0003-0099-2102

Corresponding Author(s) : Pasiyappazham Ramasamy

ramkanth281@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 1 (2025): Vol 37 Issue 1, 2025

Abstract

To study the antioxidant potential of chitosan by different in vitro methods, including chelating ability on metal ions, DPPH and superoxide radical assays. Chitin was extracted from internal bone of Sepia aculeata (cuttlebone) and through deacetylation, chitosan was prepared. The extracted chitosan was characterized with FT-IR, FESEM, XRD and exhibits a crystalline, non-porous and smooth membranous structure. The antioxidant activity was performed to determine if the isolated bioactive compounds exhibit a potency against the free radicals. Furthermore, chitosan demonstrated antioxidant activity against DPPH at 14.08–51.17% at 0.1–10 mg/mL and superoxide radicals at 24.38–67.19% at 0.1–1.6 mg/mL. At the same time, compared to EDTA, ferrous ions showed a poor chelating activity of 28.20-74.60% at concentrations of 0.1–10 mg/mL. These results indicated that cuttlebone, due to its superior antioxidant activity and ferrous ion chelating ability, can serve as an effective organic antioxidant and food additive in the pharmaceutical industry.

Keywords

Cuttlefish DPPH Chitosan Bioactive polymer Superoxide radical Antioxidant activity
Prasad, A. G., Manogaran, Y., & Ramasamy, P. (2024). Synthesis, Characterization and Antioxidant Activities of Bioactive Polymer from Internal Bone of Sepia aculeata. Asian Journal of Chemistry, 37(1), 15–18. https://doi.org/10.14233/ajchem.2025.32838
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