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Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Copyright (c) 2024 J. Sasikala, G.Subaramanian

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Bioremediation of Pharmaceutical Wastewater using Oscillatoria subsalsa and Oscillatoria flos-aquae: Efficiency in Contaminant Removal, Phytochemical Analysis and Antibacterial Activity against Biofilm-Forming Bacteria

https://doi.org/10.14233/ajchem.2024.32607
J. Sasikala
Department of Botany, Arignar Anna Government Arts College, Namakkal-637002, India
https://orcid.org/0009-0000-7029-1585
G. Subramanian
Department of Botany, Arignar Anna Government Arts College, Namakkal-637002, India
https://orcid.org/0009-0005-8444-5529

Corresponding Author(s) : G. Subramanian

gsubramanianbotany@gmail.com

Asian Journal of Chemistry, Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Abstract

This study investigates the potential of cyanobacteria, specifically Oscillatoria subsalsa and Oscillatoria flos-aquae, for the bioremediation of pharmaceutical wastewater and their effectiveness in eliminating biofilm forming, multidrug-resistant bacteria. Cyanobacteria species were collected from the Mandapam coast in India and analyzed for their ability to treat pharmaceutical effluents. Phytochemical compounds in solvent extracts were assessed prior to remediation. The cyanobacteria were evaluated for their effectiveness in reducing pH, biochemical oxygen demand (BOD), chemical oxygen demand (COD), ammonia, nitrite, nitrate and phosphate levels in wastewater. Antibacterial activity was analyzed against biofilm-forming bacterial isolates from pharmaceutical effluents. Both O. subsalsa and O. flos-aquae demonstrated significant reductions in environmental contaminants. O. subsalsa was more effective in reducing COD, while O. flos-aquae excelled in reducing nitrate and nitrite levels. Phytochemical analysis revealed the presence of alkaloids, carbohydrates, flavonoids, phenols, saponins, tannins, terpenoids, sterols and quinones, indicating a rich profile of bioactive compounds. The antibacterial tests showed that both cyanobacterial species had significant antibacterial properties, with O. flos-aquae displaying stronger and more consistent activity, particularly against Gram-negative bacteria. The study highlights the potential of O. subsalsa and O. flos-aquae in wastewater treatment and their capability to combat multidrug-resistant pathogens. These findings suggest that cyanobacteria offer a promising, sustainable approach for effective bioremediation and management of pharmaceutical wastewater.

Keywords

Cyanobacteria O. subsalsa O. flos-aquae Bioremediation Pharmaceutical wastewater Biofilm
Sasikala, J., & Subramanian, G. (2024). Bioremediation of Pharmaceutical Wastewater using Oscillatoria subsalsa and Oscillatoria flos-aquae: Efficiency in Contaminant Removal, Phytochemical Analysis and Antibacterial Activity against Biofilm-Forming Bacteria. Asian Journal of Chemistry, 36(12), 2879–2884. https://doi.org/10.14233/ajchem.2024.32607
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References
  1. A. Pereira, E.G. de Morais, L. Silva, A. Pena, A. Freitas, M.R. Teixeira, J. Varela and L. Barreira, Appl. Sci., 13, 6414 (2023); https://doi.org/10.3390/app13186414
  2. A.M.M. Azad Chowdhury and K.N. Uddin, Microbiol Insights, 15, 11786361221078211 (2022); https://doi.org/10.1177/11786361221078211
  3. I. Ahmed, M.B. Rabbi and S. Sultana, Int. J. Infect. Dis., 80, 54 (2019); https://doi.org/10.1016/j.ijid.2018.12.017
  4. S. Biswal, Muller J. Med. Sci. Res., 4, 99 (2013); https://doi.org/10.4103/0975-9727.118238
  5. S.S. Sambaza and N. Naicker, J. Glob. Antimicrob. Resist., 34, 23 (2023); https://doi.org/10.1016/j.jgar.2023.05.010
  6. M.A. Islam, M. Islam, R. Hasan, M.I. Hossain, A. Nabi, M. Rahman, W.H.F. Goessens, H.P. Endtz, A.B. Boehm and S.M. Faruque, Appl. Environ. Microbiol., 83, e00793-17 (2017); https://doi.org/10.1128/AEM.00793-17
  7. M.M. Mehanni, S.I. Gadow, F.A. Alshammari, Y. Modafer, K.Z. Ghanem, N.F. El-Tahtawi, R.F. El-Homosy and A. El-Latif Hesham, Front. Microbiol., 14, 1141383 (2023); https://doi.org/10.3389/fmicb.2023.1141383
  8. S. Akther, T. Debnath and M.M.H. Chowdhury, Adv. Biotechnol. Microbiol., 8, 555729 (2018); https://doi.org/10.19080/AIBM.2018.08.555729
  9. M. Assefa and A. Amare, Infect. Drug Resist., 15, 5061 (2022); https://doi.org/10.2147/IDR.S379502
  10. O.F.S. Khasawneh and P. Palaniandy, Process Saf. Environ. Prot., 150, 532 (2021); https://doi.org/10.1016/j.psep.2021.04.045
  11. F. Spataro, N. Ademollo, T. Pescatore, J. Rauseo and L. Patrolecco, Microchem. J., 148, 202 (2019); https://doi.org/10.1016/j.microc.2019.05.053
  12. O. Al-Mashaqbeh, L. Alsalhi, L. Salaymeh, G. Dotro and T. Lyu, Sci. Total Environ., 939, 173634 (2024); https://doi.org/10.1016/j.scitotenv.2024.173634
  13. E.P. Munzhelele, R. Mudzielwana, W.B. Ayinde and W.M. Gitari, Water, 16, 796 (2024); https://doi.org/10.3390/w16060796
  14. S. Merel, D. Walker, R. Chicana, S. Snyder, E. Baurès and O. Thomas, Environ. Int., 59, 303 (2013); https://doi.org/10.1016/j.envint.2013.06.013
  15. Z. Zahra, D.H. Choo, H. Lee and A. Parveen, Environments, 7, 13 (2020); https://doi.org/10.3390/environments7020013
  16. H. El-Sayed Touliabah, M.M. El-Sheekh, M.M. Ismail and H. El-Kassas, Molecules, 27, 1141 (2022); https://doi.org/10.3390/molecules27031141
  17. S. Dwivedi and I.Z. Ahmad, Environ. Res., 218, 114943 (2023); https://doi.org/10.1016/j.envres.2022.114943
  18. American Public Health Association (APHA), Standard Methods for the Examination of Water and Waste Water, American Public Health Association, American Water Works Association, Water Environment Federation, edn. 22 (2012).
  19. K. Meixner, C. Daffert, L. Bauer, B. Drosg and I. Fritz, Bioengineering, 9, 178 (2022); https://doi.org/10.3390/bioengineering9040178
  20. M. Pan, T. Lyu, L. Zhan, V. Matamoros, I. Angelidaki, M. Cooper and G. Pan, Water Res., 190, 116735 (2021); https://doi.org/10.1016/j.watres.2020.116735
  21. American Public Health Association (APHA), Standard Methods for the Examination of Water and Wastewater, Washington D.C. (1998).
  22. S. Jeeva, J. Marimuthu@Antonisamy, C. Domettila, B. Anantham and M. Mahesh, Asian Pacific J. Trop. Biomed., 2( Suppl. 1), S30 (2012); https://doi.org/10.1016/S2221-1691(12)60125-7
  23. S.M.M. El-Din and A.M.D. El-Ahwany, J. Taibah Univ. Sci., 10, 471 (2016); https://doi.org/10.1016/j.jtusci.2015.06.004
  24. N.S. Usha, J.V. Sabari Anand and Mangaiyarkarasi, Int. J. Basic Clin. Pharmacol., 8, 732 (2019); https://doi.org/10.18203/2319-2003.ijbcp20191108
  25. A.G. Rabiu, O.I. Falodun, J. Appl. Life Sci. Int., 13, 1 (2017); https://doi.org/10.9734/JALSI/2017/34522
  26. J. Freeman, F.R. Falkiner and C.T. Keane, J. Clin. Pathol., 42, 872 (1989); https://doi.org/10.1136/jcp.42.8.872
  27. M. Manivannan and G. Subramanian, J. Clin. Diagn. Res., 17, DC01 (2023); https://doi.org/10.7860/JCDR/2023/61200.17467
  28. P. Srimongkol, P. Sangtanoo, P. Songserm, W. Wannapawn and A. Karnchanatat, Front. Bioeng. Biotechnol., 10, 904046 (2022); https://doi.org/10.3389/fbioe.2022.904046
  29. A. Abdelfattah, S.S. Ali, H. Ramadan, E.I. El-Aswar, R. Eltawab, S.-H. Ho, T. Elsamahy, S. Li, M.M. El-Sheekh, M. Schagerl, M. Kornaros and J. Sun, Environ. Sci. Ecotechnol., 13, 100205 ( 2022); https://doi.org/10.1016/j.ese.2022.100205
  30. E.F. Shabana, H.H. Senousy, E.B. Khourshid, Egyptian J. Phycol., 20, 123 (2019); https://doi.org/10.21608/egyjs.2019.116025
  31. M.V. Jiménez-Pérez, P. Sánchez-Castillo, O. Romera, D. Fernández-Moreno and C. Pérez-Martínez, Enzyme Microb. Technol., 34, 392 (2004); https://doi.org/10.1016/j.enzmictec.2003.07.010
  32. R. Sarfraz, M. Taneez, S. Sardar, L. Danish and A. Hameed, Int. J. Environ. Anal. Chem., 103, 3575 (2021); https://doi.org/10.1080/03067319.2021.1910681
  33. V. Vanithasree and S. Murugesan, Biosci. Biotech. Res. Asia, 7, 701 (2010).
  34. N.F.Y. Tam and Y.S. Wong, Environ. Pollut., 107, 145 (2000); https://doi.org/10.1016/S0269-7491(99)00118-9
  35. S.K. Dubey, J. Dubey, S. Mehra, P. Tiwari and A.J. Bishwas, Afr. J. Biotechnol., 10, 1125 (2011).
  36. R. Amiri and M. Ahmadi, Water Environ. J., 34, 311 (2020); https://doi.org/10.1111/wej.12463
  37. J. Prarthana and K.R. Maruthi, Int. J. Adv. Res., 5, 1145 (2017).
  38. X. Cheng, J. Zheng, A. Lin, H. Xia, Z. Zhang, Q. Gao, W. Lv and H. Liu, J. Funct. Foods, 74, 104197 (2020); https://doi.org/10.1016/j.jff.2020.104197
  39. G. Deviram, T. Mathimani, S. Anto, T.S. Ahamed, D.A. Ananth and A. Pugazhendhi, J. Cleaner Prod., 253, 119770 (2020); https://doi.org/10.1016/j.jclepro.2019.119770
  40. R.Y. Ghareeb, N.R. Abdelsalam, D.M. El-Maghraby, M.H. Ghozlan, E. El-Argawy and R.A.I. Abou-Shanab, Front Plant Sci., 13, 870518 (2022); https://doi.org/10.3389/fpls.2022.870518
  41. S. Mukund, M. Muthukumaran and V. Sivasubramanian, Int. J. Pharm. Res. Dev., 6, 26 (2014).
  42. H.H. Senousy, M.M. El-Sheekh, A.A. Saber, H.M. Khairy, H.A. Said, W.A. Alhoqail and A.M. Abu-Elsaoud, Agronomy, 12, 1340 (2022); https://doi.org/10.3390/agronomy12061340
  43. M. Elumalai, G.S. Hemavathi, M. Rekha, R. Pushpalatha, A. Leelavathy, K. Vignesh, M. Ashok and M. Babu, Sensing Bio-Sensing Res., 34, 100457 (2021); https://doi.org/10.1016/j.sbsr.2021.100457
  44. Q. Wei and L.Z. Ma, Int. J. Mol. Sci., 14, 20983 (2013); https://doi.org/10.3390/ijms141020983
  45. S.S. Swain, S.K. Paidesetty and R.N. Padhy, Biomed. Pharmacother., 90, 760 (2017); https://doi.org/10.1016/j.biopha.2017.04.030
  46. S. Amudha, S. Raveendran and V. Ramamurthy, Int. J. Zool. Appl. Biosci., 1, 267 (2016).
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