Optimization and Validation of HPLC Based Analytical Method for Simultaneous Determination of Thiamethoxm Residues

Sumia Akram; Bushra Sultana; Muhammad Rafiq Aasi; Shaukat Ali; Muhammad Shahid

doi:10.14233/ajchem.2015.19083

Issue

Vol. 27 No. 11 (2015): Vol 27 Issue 11

Issue Published : July 16, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Optimization and Validation of HPLC Based Analytical Method for Simultaneous Determination of Thiamethoxm Residues

https://doi.org/10.14233/ajchem.2015.19083

Sumia Akram

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan; Institute of Food of Food Science, Cornell University, New York 14852, USA

Bushra Sultana

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

Muhammad Rafiq Aasi

Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan

Shaukat Ali

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

Muhammad Shahid

Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Corresponding Author(s) : Bushra Sultana

bushrasultana2005@yahoo.com

Asian Journal of Chemistry, Vol. 27 No. 11 (2015): Vol 27 Issue 11
Article Published : July 16, 2015

Abstract

In present study, RP-HPLC-DAD protocol was optimized and calibrated over 0-50 μg/mL of standard thiamethoxam and subsequently applied to determine its residues in selected fruits (guava and citrus) and vegetables (cauliflower, tomato and okra) after 0, 1, 7, 14, 21 and 28 days of foliar spray (0.5 mg/plant). The results regarding method validation indicated that optimized HPLC-DAD method was linear over broad range of thiamethoxam concentration (0-60 μg/mL) with recovery rates 79.64-89.30 %. Furthermore, the incidence level of thiamethoxam in selected fruits (guava and citrus) and vegetables (tomato, cauliflower and okra) was found to be higher (0.3-0.89 μg/g) than maximum residue limit (MRL) established by Codex Alimentarius Commission (0.5 μg/g) after 0 and 1 day of foliar spray. While thiamethoxam residues observed after 7, 14 and 21 of foliar application were found to be within permissible limits. The persistence of thiamethoxam in selected fruits and vegetables decreased in the following order: okra, citrus, guava, cauliflower and least in tomato. Overall, it was speculated that optimized method might be quick, easy, cheap, effective, rugged and safe (QuEChERS) choice for thiamethoxam analysis of food commodities.

Keywords

RP-HPLC-DAD Thiamethaxam Validation and Optimization LOD/LOQ Fruits and vegetables

Akram, S., Sultana, B., Rafiq Aasi, M., Ali, S., & Shahid, M. (2015). Optimization and Validation of HPLC Based Analytical Method for Simultaneous Determination of Thiamethoxm Residues. Asian Journal of Chemistry, 27(11), 4054–4058. https://doi.org/10.14233/ajchem.2015.19083

Download Citation

References

K. Aaby, D. Ekeberg and G. Skrede, J. Agric. Food Chem., 55, 4395 (2007); doi:10.1021/jf0702592.
B. Bayram, T. Esatbeyoglu, N. Schulze, B. Ozcelik, J. Frank and G. Rimbach, Plant Foods Hum. Nutr., 67, 326 (2012); doi:10.1007/s11130-012-0315-z.
K.K. Chebrolu, G.K. Jayaprakasha, K.S. Yoo, J.L. Jifon and B.S. Patil, LWT-Food Sci. Technol., 47, 449 (2012); doi:10.1016/j.lwt.2012.02.004.
V. Burmester, J. Nimptsch and C. Wiegand, Ecotoxicol. Environ. Saf., 78, 296 (2012); doi:10.1016/j.ecoenv.2011.11.037.
M. Mushtaq, B. Sultana, F. Anwar, M.Z. Khan and M. Ashrafuzzaman, Int. J. Mol. Sci., 13, 8324 (2012); doi:10.3390/ijms13078324.
M. Omirou, Z. Vryzas, E. Papadopoulou-Mourkidou and A. Economou, Food Chem., 116, 499 (2009); doi:10.1016/j.foodchem.2009.03.007.
A.Y. Ko, M.M. Rahman, A.M. Abd El-Aty, J. Jang, J.H. Park, S.K. Cho and J.H. Shim, Food Chem., 148, 402 (2014); doi:10.1016/j.foodchem.2013.10.055.
Z. Xiao, X. Li, X. Wang, J. Shen and S. Ding, J. Chromatogr. B, 879, 122 (2011); doi:10.1016/j.jchromb.2010.11.008.
X.A.N. Zhang, N. Mobley, J. Zhang, X. Zheng, L. Lu, O. Ragin and C.J. Smith, J. Agric. Food Chem., 58, 11553 (2010); doi:10.1021/jf102476m.
P. Wang, X. Yang, J. Wang, J. Cui, A.J. Dong, H.T. Zhao, L.W. Zhang, Z.Y. Wang, R.B. Xu, W.J. Li, Y.C. Zhang, H. Zhang and J. Jing, Food Chem., 134, 1691 (2012); doi:10.1016/j.foodchem.2012.03.103.
M. Tomizawa and J.E. Casida, Annu. Rev. Pharmacol. Toxicol.,45, 247 (2005); doi:10.1146/annurev.pharmtox.45.120403.095930.
K. Matsuda, M. Shimomura, M. Ihara, M. Akamatsu and D.B. Sattelle, Biosci. Biotechnol. Biochem., 69, 1442 (2005); doi:10.1271/bbb.69.1442.
S. Liu, Z. Zheng, F. Wei, Y.R. We, N. Gui, H. Wu and G. Zhu, J. Agric. Food Chem., 58, 3271 (2010); doi:10.1021/jf904045j.
R.G. Steel, J.H. Torrie and D.A. Dickey, Principles and Procedures of Statistics: A Biochemical Approach, McGraw Hill, New York, USA, edn 3 (1997).
G.T. Bakirci, D.B. YamanAcay, F. Bakirci and S. Ötles, Food Chem., 160, 379 (2014); doi:10.1016/j.foodchem.2014.02.051.
E. Dankyi, C. Gordon, D. Carboo and I.S. Fomsgaard, Sci. Total Environ., 499, 276 (2014); doi:10.1016/j.scitotenv.2014.08.051.
M.M. Jones-Lepp, J.L. Robertson and R.A. Weinzierl, J. Econ. Entomol., 105, 1431 (2010).
SANCO, 12571/2013, p. 46 (2014). Available from: http://www.eurlpesticides.eu.
Codex Alimentarius, 29 (2011).
S.B. Singh and G. Kulshrestha, Bull. Environ. Contam. Toxicol., 75, 945 (2005); doi:10.1007/s00128-005-0841-6.

References

K. Aaby, D. Ekeberg and G. Skrede, J. Agric. Food Chem., 55, 4395 (2007); doi:10.1021/jf0702592.

B. Bayram, T. Esatbeyoglu, N. Schulze, B. Ozcelik, J. Frank and G. Rimbach, Plant Foods Hum. Nutr., 67, 326 (2012); doi:10.1007/s11130-012-0315-z.

K.K. Chebrolu, G.K. Jayaprakasha, K.S. Yoo, J.L. Jifon and B.S. Patil, LWT-Food Sci. Technol., 47, 449 (2012); doi:10.1016/j.lwt.2012.02.004.

V. Burmester, J. Nimptsch and C. Wiegand, Ecotoxicol. Environ. Saf., 78, 296 (2012); doi:10.1016/j.ecoenv.2011.11.037.

M. Mushtaq, B. Sultana, F. Anwar, M.Z. Khan and M. Ashrafuzzaman, Int. J. Mol. Sci., 13, 8324 (2012); doi:10.3390/ijms13078324.

M. Omirou, Z. Vryzas, E. Papadopoulou-Mourkidou and A. Economou, Food Chem., 116, 499 (2009); doi:10.1016/j.foodchem.2009.03.007.

A.Y. Ko, M.M. Rahman, A.M. Abd El-Aty, J. Jang, J.H. Park, S.K. Cho and J.H. Shim, Food Chem., 148, 402 (2014); doi:10.1016/j.foodchem.2013.10.055.

Z. Xiao, X. Li, X. Wang, J. Shen and S. Ding, J. Chromatogr. B, 879, 122 (2011); doi:10.1016/j.jchromb.2010.11.008.

X.A.N. Zhang, N. Mobley, J. Zhang, X. Zheng, L. Lu, O. Ragin and C.J. Smith, J. Agric. Food Chem., 58, 11553 (2010); doi:10.1021/jf102476m.

P. Wang, X. Yang, J. Wang, J. Cui, A.J. Dong, H.T. Zhao, L.W. Zhang, Z.Y. Wang, R.B. Xu, W.J. Li, Y.C. Zhang, H. Zhang and J. Jing, Food Chem., 134, 1691 (2012); doi:10.1016/j.foodchem.2012.03.103.

M. Tomizawa and J.E. Casida, Annu. Rev. Pharmacol. Toxicol.,45, 247 (2005); doi:10.1146/annurev.pharmtox.45.120403.095930.

K. Matsuda, M. Shimomura, M. Ihara, M. Akamatsu and D.B. Sattelle, Biosci. Biotechnol. Biochem., 69, 1442 (2005); doi:10.1271/bbb.69.1442.

S. Liu, Z. Zheng, F. Wei, Y.R. We, N. Gui, H. Wu and G. Zhu, J. Agric. Food Chem., 58, 3271 (2010); doi:10.1021/jf904045j.

R.G. Steel, J.H. Torrie and D.A. Dickey, Principles and Procedures of Statistics: A Biochemical Approach, McGraw Hill, New York, USA, edn 3 (1997).

G.T. Bakirci, D.B. YamanAcay, F. Bakirci and S. Ötles, Food Chem., 160, 379 (2014); doi:10.1016/j.foodchem.2014.02.051.

E. Dankyi, C. Gordon, D. Carboo and I.S. Fomsgaard, Sci. Total Environ., 499, 276 (2014); doi:10.1016/j.scitotenv.2014.08.051.

M.M. Jones-Lepp, J.L. Robertson and R.A. Weinzierl, J. Econ. Entomol., 105, 1431 (2010).

SANCO, 12571/2013, p. 46 (2014). Available from: http://www.eurlpesticides.eu.

Codex Alimentarius, 29 (2011).

S.B. Singh and G. Kulshrestha, Bull. Environ. Contam. Toxicol., 75, 945 (2005); doi:10.1007/s00128-005-0841-6.

Issue

Vol. 27 No. 11 (2015): Vol 27 Issue 11

Optimization and Validation of HPLC Based Analytical Method for Simultaneous Determination of Thiamethoxm Residues

Corresponding Author(s) : Bushra Sultana

Abstract

Keywords

Full Article

Download Citation

References

Table Of Contents