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Vol. 36 No. 9 (2024): Vol 36 Issue 9, 2024

Copyright (c) 2024 Anjali Jangra, R.K. Sarin, Biswa Prakash Nayak, Himanshu Khajuria

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GC-MS/MS-Based Detection and Quantification of Methamphetamine and Mephedrone in Biological Matrices using Modified dSPE Method

https://doi.org/10.14233/ajchem.2024.32061
Anjali Jangra
Amity Institute of Forensic Sciences, Amity University, Sector-125, Noida-201301, India
https://orcid.org/0000-0002-6039-8503
R.K. Sarin
National Institute of Criminology and Forensic Science, Sector 3, Rohini, Delhi-110085, India
https://orcid.org/0000-0001-5126-1866
Biswa Prakash Nayak
Amity Institute of Forensic Sciences, Amity University, Sector-125, Noida-201301, India
https://orcid.org/0000-0002-5680-269X
Himanshu Khajuria
Amity Institute of Forensic Sciences, Amity University, Sector-125, Noida-201301, India
https://orcid.org/0000-0002-2438-0007

Corresponding Author(s) : Himanshu Khajuria

hkhajuria@amity.edu

Asian Journal of Chemistry, Vol. 36 No. 9 (2024): Vol 36 Issue 9, 2024

Abstract

This research presents a technique combining gas chromatography and tandem mass spectrometry (GC-MS/MS) with a modified dispersive solid phase extraction (D-SPE) method for identifying and quantifying methamphetamine (MTPn) and mephedrone (MPDn) in biological samples. These amphetamine-category new psychoactive substances are increasingly popular among young individuals, making their detection crucial. The method involves a clean-up procedure integrated with GC-MS/MS technology, allowing simultaneous detection of both drugs. Operating the analytical column at 40 ºC with sample concentrations ranging from 5 to 200 ng/mL, the study determined the limit of detection (7.326-20.213 ng/mL) and the limit of quantification (22.2-60.25 ng/mL). The modified dSPE method’s efficacy was compared to other extraction techniques, highlighting the sensitivity, accuracy and precision of the GC-MS/MS method. These findings have significant implications for forensic and clinical applications in detecting and quantifying methamphetamine and mephedrone.

Keywords

Psychoactive substance Mephedrone Methamphetamine Toxicology Method optimization Quantitation
Jangra, A., Sarin, R., Nayak, B. P., & Khajuria, H. (2024). GC-MS/MS-Based Detection and Quantification of Methamphetamine and Mephedrone in Biological Matrices using Modified dSPE Method. Asian Journal of Chemistry, 36(9), 2006–2012. https://doi.org/10.14233/ajchem.2024.32061
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