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Vol. 31 No. 9 (2019): Vol 31 Issue 9

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Probing into Methylene Blue Interaction with Polyglutamic Acid: Spectroscopic and Molecular Dynamics Simulation Studies

https://doi.org/10.14233/ajchem.2019.22100
Poonam Mishra Chatterjee
Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-Karnataka-576104, India; Department of Food Science and Technology, Tata Chemicals Ltd-Innovation Centre, Survey Number 315, Hissa Number 1-4, Ambedveth, Paud Road, Mulshi, Pune-412111, India
Deepika Pandey Tiwari
Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-Karnataka-576104, India; Department of Food Science and Technology, Tata Chemicals Ltd-Innovation Centre, Survey Number 315, Hissa Number 1-4, Ambedveth, Paud Road, Mulshi, Pune-412111, India
Silpi Datta
Department of Food Science and Technology, Tata Chemicals Ltd-Innovation Centre, Survey Number 315, Hissa Number 1-4, Ambedveth, Paud Road, Mulshi, Pune-412111, India
https://orcid.org/0000-0002-2272-1875
Suman Chakrabarty
School of Chemical, Biological & Macromolecular Sciences, S. N Bose National Centre for Basic Sciences, Salt Lake, Kolkata-700106, India
https://orcid.org/0000-0002-9461-0015
Ritu Raval
Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-Karnataka-576104, India
https://orcid.org/0000-0003-1297-3291
Ashok Kumar Dubey
Department of Food Science and Technology, Tata Chemicals Ltd-Innovation Centre, Survey Number 315, Hissa Number 1-4, Ambedveth, Paud Road, Mulshi, Pune-412111, India
https://orcid.org/0000-0002-1916-6655

Corresponding Author(s) : Ashok Kumar Dubey

adubey@tatachemicals.com

Asian Journal of Chemistry, Vol. 31 No. 9 (2019): Vol 31 Issue 9

Abstract

Polyglutamic acid (PGA) is an anionic biopolymer which is stained with methylene blue (MB) in agar or polyacrylamide gels for analysis. Polyglutamic acid identification involves a tedious extraction and analytical process. The molecular association of polyglutamic acid and methylene blue can be used for rapid spectroscopic detection of polyglutamic acid production during fermentation. This triggered the study on investigation of polymer-dye interaction mechanism. Concentration range of polyglutamic acid, 0.001 to 0.06 μM with a fixed methylene blue concentration of 25 μM exhibited significant differences in the spectra. Preferential higher order aggregate formation of methylene blue molecules was substantiated with molecular dynamics simulation results. Fluorescence spectroscopy demonstrated a quenching effect of polyglutamic acid on methylene blue fluorescence until a certain concentration range, beyond which the Stern-Volmer (SV) plot shows a negative deviation. Polyglutamic acid was observed to promote higher order of intramolecular stacking interactions of methylene blue, possibly due to interplay of electrostatic and hydrophobic interactions between polyglutamic acid, methylene blue alone and PGA-MB systems.

Keywords

Polyglutamic acid Methylene blue Dye Spectrophotometry
Chatterjee, P. M., Tiwari, D. P., Datta, S., Chakrabarty, S., Raval, R., & Dubey, A. K. (2019). Probing into Methylene Blue Interaction with Polyglutamic Acid: Spectroscopic and Molecular Dynamics Simulation Studies. Asian Journal of Chemistry, 31(9), 1949–1958. https://doi.org/10.14233/ajchem.2019.22100
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