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Vol. 31 No. 8 (2019): Vol 31 Issue 8

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High Performance Liquid Chromatography Analysis Using Rutin Marker and Estimation of Phenolic & Flavonoid Compounds in the Extracts of Indian Medicinal Plant Morus nigra L.

https://doi.org/10.14233/ajchem.2019.22003
Sunita Singh
Department of Pharmacy, Rameshwaram Institute of Technology & Management, Lucknow-227202, India
Alka Tripathi
Department of Applied Sciences, Institute of Engineering & Technology, Lucknow-226021, India
V.K. Lal
Lavanya Ayurveda Hospital, Chinhat, Lucknow-227105, India
Dhananjay Singh
Department of Chemical Engineering, Institute of Engineering & Technology, Lucknow-226021, India

Corresponding Author(s) : Dhananjay Singh

dsa768008@gmail.com

Asian Journal of Chemistry, Vol. 31 No. 8 (2019): Vol 31 Issue 8

Abstract

Morus nigra L. (black mulberry) belongs to moraceae family of Indian medicinal plants. Black mulberry has significant anticancerous, antioxidant, antidiabetic, antimicrobial and antiobesity activities. Rutin marker was used for high performance liquid chromatography technique. We used gallic acid and quercetin for phenolic compound determination and flavonoid content determination in plant leaf extract. Plant extract of this species was analyzed quantitatively and qualitatively through high pressure liquid chromatography. Rutin compound was the main marker, which is a flavonoidal compound. Standard solution (1 mg/mL) of rutin was prepared by using CH3OH. Methanol:water (80:20, v/v) ratio was used to dissolve powdered M. nigra plant leaves (100 mg), in which 0.01 mg/g rutin content was found. Total phenolic content in M. nigra was found 43.15 ± 0.68 mg/g GAE (gallic acid equivalents). Total flavonoid content in M. nigra was found 5.8 ± 0.46 mg/g QE. It was found that the M. nigra has not significant rutin potency and found to be as 0.01 mg/g.

Keywords

Morus nigra L. HPLC Total phenolic Flavonoids Rutin
Singh, S., Tripathi, A., Lal, V., & Singh, D. (2019). High Performance Liquid Chromatography Analysis Using Rutin Marker and Estimation of Phenolic & Flavonoid Compounds in the Extracts of Indian Medicinal Plant Morus nigra L. Asian Journal of Chemistry, 31(8), 1801–1804. https://doi.org/10.14233/ajchem.2019.22003
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