Ultrasonication Assisted Bligh and Dyer Method for Extraction of Lipids from Green Algae

R. Ghosh; R. Makam; V. Krishnamurthy; R.R. Siva Kiran; Bijay Krishna Chaudhuri; M.C. Saurav Shastry

doi:10.14233/ajchem.2019.22033

Issue

Vol. 31 No. 7 (2019): Vol 31 Issue 7

Issue Published : May 21, 2019

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ultrasonication Assisted Bligh and Dyer Method for Extraction of Lipids from Green Algae

https://doi.org/10.14233/ajchem.2019.22033

R. Ghosh

Department of Chemical Engineering, Calcutta Institute of Technology, Uluberia-711316, India

R. Makam

Department of Biotechnology, PES Univesity, Banashankari 3rd Stage, Bangalore-560085, India

V. Krishnamurthy

Department of Biotechnology, PES Univesity, Banashankari 3rd Stage, Bangalore-560085, India

R.R. Siva Kiran

Department of Chemical Engineering, M.S. Ramaiah Institute of Technology, Bangalore-560054, India

Bijay Krishna Chaudhuri

Centre For Rural & Cryogenic Technologies, Jadavpur University, Kolkata-700032, India

M.C. Saurav Shastry

Department of Chemical Engineering, M.S. Ramaiah Institute of Technology, Bangalore-560054, India

Corresponding Author(s) : R. Ghosh

rajesh_laltu@yahoo.co.in

Asian Journal of Chemistry, Vol. 31 No. 7 (2019): Vol 31 Issue 7
Article Published : June 1, 2019

Abstract

We have modified the original Bligh and Dyer method by introducing ultrasonication for enhancing the lipid extraction from green algae and compared with Soxhlet extractor. The Bligh and Dyer method coupled with ultrasonication increased the lipid extraction from green algae, Oedogonium sp. by up to 3.95 %, Botyrococcus braunii KUTZING NIES 2199 up to 8.5 %, Cladophora sp. up to 4.5 %. Therefore, ultrasonication assisted Bligh and Dyer method might be an important development for the removal of lipids from green algal biomass.

Keywords

Lipid extraction Bligh and Dyer method Green algae Botyrococcus braunii Oedogonium sp. Cladophora sp.

Ghosh, R., Makam, R., Krishnamurthy, V., Siva Kiran, R., Chaudhuri, B. K., & Saurav Shastry, M. (2019). Ultrasonication Assisted Bligh and Dyer Method for Extraction of Lipids from Green Algae. Asian Journal of Chemistry, 31(7), 1555–1557. https://doi.org/10.14233/ajchem.2019.22033

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References

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A.S. Hossain, A. Salleh, A.N. Boyce, P. Chowdhury and M. Naqiuddin, Am. J. Biochem. Biotechnol., 4, 250 (2008); https://doi.org/10.3844/ajbbsp.2008.250.254.

A.H. Hirani, N. Javed, M. Asif, S.K. Basu and A. Kumar, eds.: A. Kumar, S. Ogita and Y.Y. Yau, A Review on First- and Second-Generation Biofuel Productions, In: Biofuels: Greenhouse Gas Mitigation and Global Warming, Springer: New Delhi (2018).

S.N. Naik, V.V. Goud, P.K. Rout and A.K. Dalai, Renew. Sustain. Energy Rev., 14, 578 (2010); https://doi.org/10.1016/j.rser.2009.10.003.

A.K. Bajhaiya, S.K. Mandotra, M.R. Suseela, K. Toppo and S. Ranade, Asian J. Exp. Biol. Sci., 1, 728 (2010).

M.P. Abishek, J. Patel and A.P. Rajan, Biotechnol. Res. Int., 2014, Article ID 272814 (2014); https://doi.org/10.1155/2014/272814.

G. Khola and B. Ghazala, Pak. J. Bot., 44, 379 (2011).

M. Gavrilescu and Y. Chisti, Biotechnol. Adv., 23, 471 (2005); https://doi.org/10.1016/j.biotechadv.2005.03.004.

E.S. Jang, M.Y. Jung and D.B. Min, Comp. Rev. Food Sci., 4, 22 (2005); https://doi.org/10.1111/j.1541-4337.2005.tb00069.x.

J. Sheehan, T. Dunahay, J. Benemann and P. Roessler, A Look Back at the U.S. Department of Energy’s Aquatic Species Program: Biodiesel from Algae, Close-Out Report, United States Department of Energy July (1998).

K. Georgiú and V. Mari, Food Technol. Biotechnol., 39, 175 (2001).

S. Elumalai, R. Sakthivel and S.G. Kumar, Curr. Botany, 2, 19 (2011).

B. Blagovi, M. Mesari, V. Mari and J. Rup, Croat. Chem. Acta, 78, 479 (2005).

W. Zhang, Y. Zhao, B. Cui, H. Wang and T. Liu, Bioresour. Technol., 220, 407 (2016); https://doi.org/10.1016/j.biortech.2016.08.106.

P. Metzger and C. Largeau, Appl. Microbiol. Biotechnol., 66, 486 (2005); https://doi.org/10.1007/s00253-004-1779-z.

A.M. Illman, A.H. Scragg and S.W. Shales, Enzyme Microb. Technol., 27, 631 (2000); https://doi.org/10.1016/S0141-0229(00)00266-0.

A. Ranjan, C. Patil and V.S. Moholkar, Ind. Eng. Chem. Res., 49, 2979 (2010); https://doi.org/10.1021/ie9016557.

D.F. Dall'Oglio, L.C. de Sousa, S.A. de Sousa, M.A. Garcia, E.S. Sousa, S.G. de Lima and C.V. de Moura, J. Braz. Chem. Soc., 30, 633 (2019); https://doi.org/10.21577/0103-5053.20180235.

K. Yamaguchi, H. Nakano, M. Murakami, S. Konosu, O. Nakayama, M. Kanda and H. Iwamoto, Agric. Biol. Chem., 51, 493 (1987); https://doi.org/10.1271/bbb1961.51.493.

E.G. Bligh, and W.J. Dyer, Can. J. Biochem. Physiol., 37, 911 (1959); https://doi.org/10.1139/o59-099.

L. Xiao, Ph.D. Thesis, Evaluation of Extraction Methods for Recovery of Fatty Acids from Marine Products, University of Bergen (2010). https://sapientia.ualg.pt/handle/10400.1/10737.

Issue

Vol. 31 No. 7 (2019): Vol 31 Issue 7

Ultrasonication Assisted Bligh and Dyer Method for Extraction of Lipids from Green Algae

Corresponding Author(s) : R. Ghosh

Abstract

Keywords

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