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Vol. 31 No. 6 (2019): Vol 31 Issue 6

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Isolation, Characterization and Assessment for Central Nervous System Effects of Novel Phytomolecule from Galphimia glauca Cav. Stems

https://doi.org/10.14233/ajchem.2019.21748
Vaishnavi Veera Reddy
Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Medchal District-501301, India
Bhargavi Thalluri
Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Medchal District-501301, India
Baba Shankar Rao Garige
Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Medchal District-501301, India
Ram Mohan Manda
Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Medchal District-501301, India
Vasudha Bakshi
Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Medchal District-501301, India

Corresponding Author(s) : Baba Shankar Rao Garige

babaphd2010@gmail.com

Asian Journal of Chemistry, Vol. 31 No. 6 (2019): Vol 31 Issue 6

Abstract

The investigation had explored the in vivo central nervous system (CNS) effects of novel isolated phytoconstituent from Galphimia glauca stems. The isolated molecule coded as “BS-2” was subjected to toxicological, central analgesic and central nervous system depressant studies on Swiss albino mice and Wistar rats. The LD-50 of BS-2 was found to be > 2000 mg/kg. The mice treated with BS-2 at 12.5, 25 and 50 mg/kg doses exhibited significant effect in the hot plate test (P < 0.5) and tail clip test (83 % inhibition). Mice treated with BS-2 prolonged the time of sleep induced by sodium pentobarbital (40 mg/kg, i.p.). Throughout the hole-board experiment and open field test the BS-2 at 50 mg/kg exhibited a significant (P ≤ 0.001) dose-dependent activity. The BS-2 was characterized by Rf value, melting point, IR spectra, mass spectra and 1H NMR spectrum. The BS-2 showed potential in vivo central pain relieving and central nervous system depressant effects.

Keywords

Column chromatography Galphimia glauca Depressant effects
Reddy, V. V., Thalluri, B., Rao Garige, B. S., Manda, R. M., & Bakshi, V. (2019). Isolation, Characterization and Assessment for Central Nervous System Effects of Novel Phytomolecule from Galphimia glauca Cav. Stems. Asian Journal of Chemistry, 31(6), 1230–1236. https://doi.org/10.14233/ajchem.2019.21748
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