Identification and Antibacterial Activities of Composition Compounds from Liver and Texture of Hypophthalmichtys molitrix

Abstract

In this investigation, the liver and muscle tissues of Hypophthalmichtys molitrix were extracted of their lipid and fatty acid contents using the method of Bligh & Dyer. Chloroform and n-butanol were used as extraction solvents for semi and polar lipids, respectively. After removal of the solvents, the GC/MS spectra of the fractions were obtained. Identification of constituents was made by comparison of their mass spectra and retention indices (RI) with those given in the literature and authentic samples. Seven compounds representing 99.8 % of the liver chloroform phase of Hypophthalmichtys molitrix were identified among them palmitic acid (16.7 %) and cholesta-3,5-diene (42.5 %) were the major ones. Eight compounds representing 97. 9 % of tissue chloroform phase were identified among them palmitic acid (26 %) and oleic acid (12 %) were the major ones. One polar compound representing 99 % of the liver n-butanol phase and no polar compound representing of muscle n-butanol phase. Two antibacterial activities were reported in tissue choloroform phase.