Correlation Between Cytokinins and Polyamines Contents of Capsicum annuum L. Callus Cultivated in vitro

Abstract

Polyamines are naturally found both in animals and plants. They play important roles in a number of cellular processes such as replication and translation, embriyonic development, cell cycle, programmed cell death and cancer. Although the metabolic pathway of polyamines is lighten in recent years, its relationship with hormones still remains unclear. In this study, the authors investigate a correlation between polyamines and hormones. Endogen cytokinins and polyamines (spermidine and spermine) contents have determined by TLC and HPLC methods in Capsicum annuum L. subcallus that derived from callus formation medium consist of arginine, 2,4-Dichlorophenoxyacetic acid (2,4-D) and both of them at day 21. Phenylalanine amonium liyas (PAL) enzyme analysis have also performed. The results suggest that the use of cytokinins in cell may only related to spermine. Polyamines amount was been proportional to decrease of PAL enzyme content. When cytokinin and PAL enzyme content was compared, the consumption of cytokinin in callus which placed applicaton medium, was likely to decrease PAL activity.The results suggest that the use of cytokinins in cell may only related to spermine.