Synthesis and in vitro Cytotoxic Activity of Isatin Derivatives

Abstract

The objective of the study was to synthesize a novel series of 3-substituted isatin derivative by reacting substituted isatin with amino derivatives of lamotrigine. The Schiff base is further condensed with secondary amine and formaldehyde (mannich reaction). N-acetyl derivatives of isatin were prepared using acetic anhydride. The structure of synthesized compounds was elucidated by spectral analysis. The synthesized compounds were studied for its in vitro cytotoxic potential against two cancerous cell lines viz., HEp-2 and DLA cells by standard procedures. All the compounds exhibited cytotoxic properties and compound 5c exhibited comparatively more cytotoxic activity. The results obtained from the present study show the compound are cytotoxic in nature and may posses antitumor activity.