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Antioxidant Activity, Phenol and Flavonoid Contents of Indonesian Propolis
Corresponding Author(s) : SYAMSUDIN
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
In present work, a systematic study of the relative antioxidant activity of Indonesian propolis collected from different regions of Java have been reported. The total phenol varied from 136.2 ± 10.1 to 203.7 ± 9.8 mg g-1 in the extracts. Flavones and flavonols were determined using aluminium chloride and expressed as quercetine equivalent, while flavanones were determined using 2,4-dinitrophenylhydrazine and expressed as naringenin. Contents of flavones and flavonols were similar for most samples and ranged from 1.7-7.3 %. The content of flavanones in propolis samples varies from 1.2-5.9 %. 1,1-Diphenyl-2-picrylhydrazine (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. IC50 of the extracts and the standard compounds quercetine was 8.44, 130.35, 19.27, 91.03 and 2.58 μg mL-1.
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