Issue

Vol. 36 No. 7 (2024): Vol 36 Issue 7, 2024

Copyright (c) 2024 Janitha Kumarathunga, Sithumini Chathurangi, Sandamali Rajapaksha, Chandula Sooriyawansha, Shehan Jayawardena, Harsha Dananjaya, Dinithi Alwis, chamila kadigamuwa, jayangika dahanayake, Suranga Wickramarachchi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

In vivo and in silico Antifungal Activity of Cinnamon Leaf Oil and Lemongrass Oil containing Chitosan Microcapsules against Aspergillus flavus

https://doi.org/10.14233/ajchem.2024.31750
P.G.J.D. Kumarathunga
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0008-6756-603X
S. Chathurangi
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0002-8047-9121
R.P.S.P. Rajapaksha
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0000-8174-8747
A.M.S.C. Sooriyawansha
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0007-8597-7847
P.A.S.N.P. Jayawardena
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0005-3579-1134
P.D.H. Dananjaya
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0000-1796-105X
M.D.N. Alwis
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0009-0009-4743-6040
C.C. Kadigamuwa
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0000-0001-8210-9533
J.N. Dahanayake
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0000-0002-1867-4324
S.R. Wickramarachchi
Department of Chemistry, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
https://orcid.org/0000-0001-7653-332X

Corresponding Author(s) : S.R. Wickramarachchi

suranga@kln.ac.lk

Asian Journal of Chemistry, Vol. 36 No. 7 (2024): Vol 36 Issue 7, 2024

Abstract

This study aimed to examine the potentiality of microencapsulated cinnamon leaf oil (CNO-CS-MCs) and lemongrass oil (LGO-CS-MCs) as natural fungicides against Aspergillus flavus. Oil encapsulated microcapsules were synthesized using ionotropic gelation method. Cinnamon leaf oil (CNO) and lemongrass oil (LGO) were characterized using GC-MS. A. flavus was isolated and identified using DNA sequencing. The minimum inhibitory and minimum lethal doses of oil-loaded microcapsules against A. flavus were evaluated under in vivo conditions and the results were further confirmed by in silico analysis. The major constituents of CNO and LGO were eugenol and citral, respectively. The minimum inhibitory doses of CNO-CS-MCs and LGO-CS-MCs were 5 mg and 7.5 mg, respectively. The minimum lethal dose of CNO-CS-MCs was 12.5 mg. As CNO showed considerably high antifungal activity than LGO, Computational investigations were carried out on the action of CNO against A. flavus. The highest protein-ligand interaction was observed for squalene epoxidase (SQ)-benzyl benzoate (BEN) complex with the binding energy of -7.70 kcal/mol. Molecular dynamics simulations were performed on SQ-BEN complex for 10 ns using CHARMM36 force field. The Rg, RMSD and RMSF results indicated the stabilization of the SQ-BEN complex throughout the simulation time.

Keywords

Aspergillus flavus Chitosan Cinnamon leaf oil Lemongrass oil Microcapsules
Kumarathunga, P., Chathurangi, S., Rajapaksha, R., Sooriyawansha, A., Jayawardena, P., Dananjaya, P., … Wickramarachchi, S. (2024). In vivo and in silico Antifungal Activity of Cinnamon Leaf Oil and Lemongrass Oil containing Chitosan Microcapsules against Aspergillus flavus. Asian Journal of Chemistry, 36(7), 1633–1640. https://doi.org/10.14233/ajchem.2024.31750
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. J.W.P. Kumari, L.K.W. Wijayaratne, N.W.I.A. Jayawardena and W.C.P. Egodawatta, Sri Lankan J. Agric. Ecosyst., 2, 99 (2020); https://doi.org/10.4038/sljae.v2i1.32
  2. M. Mannaa and K.D. Kim, Mycobiology, 46, 287 (2018); https://doi.org/10.1080/12298093.2018.1505247
  3. J.M.R.S. Bandara, A.K. Vithanege and G.A. Bean, Mycopathologia, 115, 31 (1991); https://doi.org/10.1007/BF00436418
  4. S. Nayak, U. Dhua and S. Samanta, Int. J. Curr. Microbiol. Appl. Sci., 3, 170 (2014).
  5. M. Mannaa and K.D. Kim, Mycobiology, 44, 67 (2016); https://doi.org/10.5941/MYCO.2016.44.2.67
  6. A. Sharma, V. Kumar, B. Shahzad, M. Tanveer, G.P.S. Sidhu, N. Handa, S.K. Kohli, P. Yadav, A.S. Bali, R.D. Parihar, O.I. Dar, K. Singh, S. Jasrotia, P. Bakshi, M. Ramakrishnan, S. Kumar, R. Bhardwaj and A.K. Thukral, SN Appl. Sci., 1, 1446 (2019); https://doi.org/10.1007/s42452-019-1485-1
  7. G.M.W. Lengai, J.W. Muthomi and E.R. Mbega, Sci. Afr., 7, e00239 (2020); https://doi.org/10.1016/j.sciaf.2019.e00239
  8. Y. Xing, X. Li, Q. Xu, J. Yun and Y. Lu, Int. J. Food Sci. Technol., 45, 1837 (2010); https://doi.org/10.1111/j.1365-2621.2010.02342.x
  9. S. Ali and A. Yousef, Int. J. Curr. Microbiol. Appl. Sci., 2, 261 (2013).
  10. E.A. Soliman, A.Y. El-Moghazy, M.S.M. El-Din and M.A. Massoud, J. Encapsulation Adsorpt. Sci., 3, 48 (2013); https://doi.org/10.4236/jeas.2013.31006
  11. N.V.N. Jyothi, P.M. Prasanna, S.N. Sakarkar, K.S. Prabha, P.S. Ramaiah and G.Y. Srawan, J. Microencapsul., 27, 187 (2010); https://doi.org/10.3109/02652040903131301
  12. N. Eghbal, W. Liao, E. Dumas, S. Azabou, P. Dantigny and A. Gharsallaoui, Appl. Sci., 12, 3837 (2022); https://doi.org/10.3390/app12083837
  13. L. Tian, T. Qiang, C. Liang, X. Ren, M. Jia, J. Zhang, J. Li, M. Wan, X. YuWen, H. Li, W. Cao and H. Liu, Eur. J. Med. Chem., 213, 113201 (2021); https://doi.org/10.1016/j.ejmech.2021.113201
  14. L.M. Douglas and J.B. Konopka, Annu. Rev. Microbiol., 68, 377 (2014); https://doi.org/10.1146/annurev-micro-091313-103507
  15. S. Dhingra and R.A. Cramer, Front. Microbiol., 8, (2017); https://doi.org/10.3389/fmicb.2017.00092
  16. M.D. Lenardon, C.A. Munro and N.A.R. Gow, Curr. Opin. Microbiol., 13, 416 (2010); https://doi.org/10.1016/j.mib.2010.05.002
  17. F. Trail, P.K. Chang, J. Cary and J.E. Linz, Appl. Environ. Microbiol., 60, 4078 (1994); https://doi.org/10.1128/aem.60.11.4078-4085.1994
  18. K.C. Ehrlich, Toxins, 1, 37 (2009); https://doi.org/10.3390/toxins1010037
  19. P.P. Singh, A.K. Jaiswal, A. Kumar, V. Gupta and B. Prakash, Sci. Rep., 11, 6832 (2021); https://doi.org/10.1038/s41598-021-86253-8
  20. F. Tian, S.Y. Woo, S.Y. Lee, S.B. Park, Y. Zheng and H.S. Chun, Antibiotics, 11, 1727 (2022); https://doi.org/10.3390/antibiotics11121727
  21. Y.L. Paragodaarachchi, P. Subasinghe and S.R. Wickramarachchi, Acta Chem. Iasi., 28, 129 (2020); https://doi.org/10.2478/achi-2020-0009
  22. U.G.P.P. Subasinghe and S. Wickramarachchi, Ceylon J. Sci., 48, 279 (2019); https://doi.org/10.4038/cjs.v48i3.7652
  23. H.A. Seepe, K.E. Lodama, R. Sutherland, W. Nxumalo and S.O. Amoo, Plants, 9, 1668 (2020); https://doi.org/10.3390/plants9121668
  24. N. Hansini, A.M.S.C. Sooriyawansha, P.A.S.N.P. Jayawardena, P.G.J.D. Kumarathunga, P.D.H. Dananjaya, E.A.C.P. Edirisinghe, M.D.N. Alwis, D.A. Daranagama, J.N. Dahanayake and C.C. Kadigamuwa, Med. Plants - Int. J. Phytomedicines Relat. Ind., 15, 677 (2023); https://doi.org/10.5958/0975-6892.2023.00068.0
  25. R. Khan, F. Mohamad Ghazali, N.A. Mahyudin and N.I.P. Samsudin, Agriculture, 10, 450 (2020); https://doi.org/10.3390/agriculture10100450
Read More
Author biographies is not available.
Statistic
Read Counter : 0 Download : 0