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Vol. 36 No. 5 (2024): Vol 36 Issue 5, 2024

Copyright (c) 2024 Samriddh Srivastava, Garima Mathur

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Production and Characterization of Bacterial Cellulose Synthesized by Komagataeibacter sp. Isolated from Rotten Coconut Pulp

https://doi.org/10.14233/ajchem.2024.31470
Samriddh Srivastava
Plant and Microbial Biotechnology Centre, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sec-62, Noida-201309, India
https://orcid.org/0000-0002-6333-3641
Garima Mathur
Plant and Microbial Biotechnology Centre, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sec-62, Noida-201309, India
https://orcid.org/0000-0001-5216-4849

Corresponding Author(s) : Garima Mathur

garimacity@gmail.com

Asian Journal of Chemistry, Vol. 36 No. 5 (2024): Vol 36 Issue 5, 2024

Abstract

Bacterial cellulose is a naturally occurring polysaccharide that is produced by acetic acid bacteria. It has become increasingly popular due to its numerous biotechnological applications. Bacterial cellulose is free from contaminants that are often associated with plant cellulose. This study aims at isolation of bacterial cellulose producing microbial strains from rotten fruits. The selected bacterial cellulose producer was identified using biochemical tests, including biochemical, carbohydrate fermentation, morphology, gram staining and 16S rRNA sequence analysis. Furthermore, several different media were evaluated for higher production of cellulose. The produced cellulose sheets were washed, purified and characterized using various analytical techniques, including FTIR, XRD and SEM analysis. A potential high bacterial cellulose yielding microbial strain was isolated from coconut pulp waste and identified as Komagataeibacter saccharivorans strain BC-C1 using 16S rRNA sequencing. Effect of media components on bacterial cellulose production was studied. The isolate yielded 0.52 g/100 mL with Hestrin-Schramm (HS) culture media (designated as M1) and 1.10 g/100 mL in M5 media. The physico-chemical characterization demonstrated that produced bacterial cellulose sheets show characteristics IR bands and three-dimensional fibrillar interconnected network structure. The study revealed that isolated Komagataeibacter saccharivorans strain BC-C1 can be utilized for large scale production of bacterial cellulose.

Keywords

Bacterial cellulose Coconut Komagataeibacter Rotten fruits
Srivastava, S., & Mathur, G. (2024). Production and Characterization of Bacterial Cellulose Synthesized by Komagataeibacter sp. Isolated from Rotten Coconut Pulp. Asian Journal of Chemistry, 36(5), 1183–1190. https://doi.org/10.14233/ajchem.2024.31470
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References
  1. G. Buldum and A. Mantalaris, Int. J. Mol. Sci., 22, 7192 (2021); https://doi.org/10.3390/ijms22137192
  2. S.P. Nunes, P.Z. Culfaz-Emecen, G.Z. Ramon, T. Visser, G.H. Koops, W. Jin and M. Ulbricht, J. Membr. Sci., 598, 117761 (2020); https://doi.org/10.1016/j.memsci.2019.117761
  3. Z. Wu, S. Chen, J. Li, B. Wang, M. Jin, Q. Liang, D. Zhang, Z. Han, L. Deng, X. Qu and H. Wang, Adv. Funct Mater., 33, 2214327 (2023); https://doi.org/10.1002/adfm.202214327
  4. P. Sintharm, A. Nimpaiboon, Y.C. Liao and M. Phisalaphong, Cellulose, 29, 1739 (2022); https://doi.org/10.1007/s10570-021-04366-9
  5. S. Gorgieva and J. Trèek, Nanomaterials, 9, 1352 (2019); https://doi.org/10.3390/nano9101352
  6. G. Takayama and T. Kondo, Carbohydr. Polym., 321, 121311 (2023); https://doi.org/10.1016/j.carbpol.2023.121311
  7. L. Urbina, M.Á. Corcuera, N. Gabilondo, A. Eceiza and A. Retegi, Cellulose, 28, 8229 (2021); https://doi.org/10.1007/s10570-021-04020-4
  8. N. Pogorelova, E. Rogachev, I. Digel, S. Chernigova and D. Nardin, Materials, 13, 2849 (2020); https://doi.org/10.3390/ma13122849
  9. S. Swingler, A. Gupta, H. Gibson, M. Kowalczuk, W. Heaselgrave and I. Radecka, Polymers, 13, 412 (2021); https://doi.org/10.3390/polym13030412
  10. M.A. Dutt, M.A. Hanif, F. Nadeem and H.N. Bhatti, J. Environ. Chem. Eng., 8, 104073 (2020); https://doi.org/10.1016/j.jece.2020.104073
  11. C. Campano, A. Balea, A. Blanco and C. Negro, Cellulose, 23, 57 (2016); https://doi.org/10.1007/s10570-015-0802-0
  12. A. Adnan, G. Nair, M. Lay and J. Swan, Trends Sci., 18, 453 (2021); https://doi.org/10.48048/tis.2021.453
  13. R.R. Singhania, A.K. Patel, M.L. Tsai, C.W. Chen and C.D. Dong, Bioengineered, 12, 6793 (2021); https://doi.org/10.1080/21655979.2021.1968989
  14. T. Kamal, M. Ul-Islam, A. Fatima, M.W. Ullah and S. Manan, Gels, 8, 552 (2022); https://doi.org/10.3390/gels8090552
  15. M.R. Kosseva, S. Zhong, M. Li, J. Zhang and N.A. Tjutju, Biopolymers Produced from Food Wastes: A Case Study on Biosynthesis of Bacterial Cellulose from Fruit Juices, In: Food Industry Wastes, Academic Press, pp. 225-254 (2020).
  16. M. Velásquez-Riaño and V. Bojacá, Cellulose, 24, 2677 (2017); https://doi.org/10.1007/s10570-017-1309-7
  17. B.E. Rangaswamy, K.P. Vanitha and B.S. Hungund, Int. J. Polym. Sci., 2015, 280784 (2015); https://doi.org/10.1155/2015/280784
  18. G. Gopu and S. Govindan, Prep. Biochem. Biotechnol., 48, 842 (2018); https://doi.org/10.1080/10826068.2018.1513032
  19. G.M. Garrity, Bergey’s Manual of Systematic Bacteriology, Springer: New York, edn. 2, pp. 41-43 (2002).
  20. F. Mohammadkazemi, M. Azin and A. Ashori, Carbohydr. Polym., 117, 518 (2015); https://doi.org/10.1016/j.carbpol.2014.10.008
  21. M.S. Dayal, N. Goswami, A. Sahai, V. Jain, G. Mathur and A. Mathur, Carbohydr. Polym., 94, 12 (2013); https://doi.org/10.1016/j.carbpol.2013.01.018
  22. P.J.M.P. Scherrer, J. Math. Phys., 2, 98 (1918).
  23. C. Molina-Ramírez, C. Castro, R. Zuluaga and P. Ganan, J. Polym. Environ., 26, 830 (2018); https://doi.org/10.1007/s10924-017-0993-6
  24. R. Srinivasan, U. Karaoz, M. Volegova, J. MacKichan, M. Kato-Maeda, S. Miller, R. Nadarajan, E.L. Brodie and S.V. Lynch, PLoS One, 10, e0117617 (2015); https://doi.org/10.1371/journal.pone.0117617
  25. S. La China, G. Zanichelli, L. De Vero and M. Gullo, Biotechnol. Lett., 40, 1289 (2018); https://doi.org/10.1007/s10529-018-2591-7
  26. D. Ciolacu, F. Ciolacu and V.I. Popa, Cellul. Chem. Technol., 45, 13 (2011).
  27. M. Ul-Islam, M.W. Ullah, S. Khan and J.K. Park, Korean J. Chem. Eng., 37, 925 (2020); https://doi.org/10.1007/s11814-020-0524-3
  28. S. Gorgieva, U. Jancic, E. Cepec and J. Trcek, Int. J. Biol. Macromol., 244, 125368 (2023); https://doi.org/10.1016/j.ijbiomac.2023.125368
  29. S.-S. Wang, Y.-H. Han, J.-L. Chen, D.-C. Zhang, X.-X. Shi, Y.-X. Ye, D.-L. Chen and M. Li, Polymers, 10, 963 (2018); https://doi.org/10.3390/polym10090963
  30. M.E. Fuller, C. Andaya and K. McClay, J. Microbiol. Methods, 144, 145 (2018); https://doi.org/10.1016/j.mimet.2017.10.017
  31. M.O. Akintunde, B.C. Adebayo-Tayo, M.M. Ishola, A. Zamani and I.S. Horváth, Bioengineered, 13, 10010 (2022); https://doi.org/10.1080/21655979.2022.2062970
  32. G. Paladini, V. Venuti, V. Crupi, D. Majolino, A. Fiorati and C. Punta, Polymers, 13, 528 (2021); https://doi.org/10.3390/polym13040528
  33. C. Fosse, A. Esposito, P. Lemechko, Y.S. Salim, V. Causin, V. Gaucher, S. Bruzaud, K. Sudesh, L. Delbreilh and E. Dargent, J. Polym. Environ., 31, 4430 (2023); https://doi.org/10.1007/s10924-023-02882-2
  34. L. Chen, F. Hong, X.X. Yang and S.F. Han, Bioresour. Technol., 135, 464 (2013); https://doi.org/10.1016/j.biortech.2012.10.029
  35. H. Seddiqi, E. Oliaei, H. Honarkar, J. Jin, L.C. Geonzon, R.G. Bacabac and J. Klein-Nulend, Cellulose, 28, 1893 (2021); https://doi.org/10.1007/s10570-020-03674-w
  36. J. Kang, X. Yang, Q. Hu, Z. Cai, L.M. Liu and L. Guo, Chem. Rev., 123, 8859 (2023); https://doi.org/10.1021/acs.chemrev.3c00229
  37. Z. Peter, Carbohydr. Polym., 254, 117417 (2021); https://doi.org/10.1016/j.carbpol.2020.117417
  38. M.T. Shaaban, M. Zayed and H.S. Salama, Curr. Microbiol., 80, 75 (2023); https://doi.org/10.1007/s00284-023-03182-7
  39. N.F. Vasconcelos, J.P.A. Feitosa, F.M.P. da Gama, J.P.S. Morais, F.K. Andrade, M.S.M. de Souza Filho and M.F. Rosa, Carbohydr. Polym., 155, 425 (2017); https://doi.org/10.1016/j.carbpol.2016.08.090
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