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Vol. 36 No. 6 (2024): Vol 36 Issue 6, 2024

Copyright (c) 2024 Dyah Iswantini, Evul Winoto, Budhi Antariksa, Mohamad Rafi, Setyanto Tri Wahyudi

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Metabolite Profiling and Inhibitory Effects of Nitric Oxide on Andrographis paniculata Burm. F Nees Extract using Different Solvents as a Potential Candidate in COVID-19 Therapy

https://doi.org/10.14233/ajchem.2024.31387
Evul Winoto Lukito
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia
https://orcid.org/0009-0008-2112-2652
Dyah Iswantini
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia
Budhi Antariksa
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia
Mohamad Rafi
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia
Setyanto Tri Wahyudi
Department of Physics, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia

Corresponding Author(s) : Dyah Iswantini

dyahis@apps.ipb.ac.id

Asian Journal of Chemistry, Vol. 36 No. 6 (2024): Vol 36 Issue 6, 2024

Abstract

Andrographis paniculata Burm. F Nees used to treat COVID-19 in several Asian countries because it has metabolites with pharmacological activity that may decrease the pathogenesis features of COVID-19. The extraction solvent significantly affected the composition and concentration of the metabolites. Therefore, the inhibitory effects of nitric oxide of A. paniculata extract based on differences in extraction solvent concentrations were investigare and also identified its metabolite profile. The leaves of A. paniculata were extracted with three different solvents like water solvent, 50% ethanol and pure ethanol. Nitrite oxide (NO) inhibitory activity on LPS-activated RAW 264.7 macrophage cells was used to evaluate the anti-inflammatory activity of the extract, the identification of metabolite profile using LC-MS/MS. Extract pure ethanol had the highest inhibition potential against NO at 91.02%, whereas water and 50% ethanol solvents had inhibitory potentials of 21.35% and -8.13%, respectively. The metabolites 14-deoxyandrographoside and 14-deoxy-17-hydroxyandrographolid were only identified in the pure ethanol extract. This study shows that differences in solvent concentrations play an essential role in enhancing the pharmacological potential of A. paniculata as an anti-inflammatory candidate for COVID-19 treatment.

Keywords

Andrographis paniculata Nitrite oxide COVID-19 Extraction LC-MS/MS
Lukito, E. W., Iswantini, D., Antariksa, B., Rafi, M., & Wahyudi, S. T. (2024). Metabolite Profiling and Inhibitory Effects of Nitric Oxide on Andrographis paniculata Burm. F Nees Extract using Different Solvents as a Potential Candidate in COVID-19 Therapy. Asian Journal of Chemistry, 36(6), 1301–1307. https://doi.org/10.14233/ajchem.2024.31387
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