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Vol. 36 No. 5 (2024): Vol 36 Issue 5, 2024

Copyright (c) 2024 Prabakar Kandasamy, Chinnaraju Chinnasamy , Madhu Ganesan Chinnaperamanoor, Lavanya A R

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Antibacterial Activity of Silver Nanoparticles Synthesized from Endophytic Streptomyces sp. CR 13 Isolated from the Roots of Ocimum tenuiflorum L.

https://doi.org/10.14233/ajchem.2024.31361
Chinnasamy Chinnaraju
P.G. & Research Department of Botany, Thanthai Periyar Government Arts and Science College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620023, India
https://orcid.org/0000-0002-1163-5146
Chinnaperamanoor Madhu Ganesan
P.G. & Research Department of Zoology, Jamal Mohamed College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620020, India
https://orcid.org/0000-0003-3173-0068
Kandasamy Prabakar
P.G. & Research Department of Zoology, Jamal Mohamed College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620020, India
https://orcid.org/0000-0002-7055-7299
A.R. Lavanya
P.G. & Research Department of Botany, Thanthai Periyar Government Arts and Science College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620023, India
https://orcid.org/0000-0001-5034-4512

Corresponding Author(s) : A.R. Lavanya

lavanyaar2021@gmail.com

Asian Journal of Chemistry, Vol. 36 No. 5 (2024): Vol 36 Issue 5, 2024

Abstract

In this study, Streptomyces sp. CR 13 from the roots of Ocimum tenuiflorum L. was isolated and characterized using biochemical, morphological and genomic approaches. Silver nanoparticles (AgNPs) were synthesized extracellularly using cell-free extract and characterized with UV-vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), EDX, DLS and zeta potential techniques. The maximum peak of UV-visible spectroscopy was observed at 425 nm and the TEM images revealed that the nanoparticles were spherical in shape and ranged in size from 18 to 21 nm. DLS analysis revealed particles with an average diameter of 147.9 nm. The zeta potential analysis of biosynthesized AgNPs showed a peak at -35.2 mV, indicating their high stability. Moreover, AgNPs synthesized by endophytic Streptomyces showed significant antimicrobial activity against Escherichia coli, Klebsiella pneumonia and Stphylococcus aureus.

Keywords

Endophyte Streptomyces Ocimum tenuiflorum L. Silver nanoparticles Antibacterial activity
Chinnaraju, C., Ganesan, C. M., Prabakar, K., & Lavanya, A. (2024). Antibacterial Activity of Silver Nanoparticles Synthesized from Endophytic Streptomyces sp. CR 13 Isolated from the Roots of Ocimum tenuiflorum L. Asian Journal of Chemistry, 36(5), 1101–1108. https://doi.org/10.14233/ajchem.2024.31361
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