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Vol. 31 No. 7 (2019): Vol 31 Issue 7

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Green Synthesis of Silver Nanoparticles from Oxynema thaianum ALU PBC5 and their in vitro and in vivo Activity Against ESBL Producing MDR Escherichia coli and Klebsiella pneumoniae

https://doi.org/10.14233/ajchem.2019.21821
Nagarajan Padmini
Department of Microbiology, Science campus, Alagappa University, Karaikudi-630003, India
Nagasundaram Rashiya
Department of Microbiology, Science campus, Alagappa University, Karaikudi-630003, India
Natesan Sivakumar
School of Biotechnology, Madurai Kamaraj University, Madurai-625021, India
Narayanan Dhiraviam Kannan
School of Biotechnology, Madurai Kamaraj University, Madurai-625021, India
Ramamoorthy Manjuladevi
School of Biotechnology, Madurai Kamaraj University, Madurai-625021, India
Periyannan Rajasekar
Department of Animal Health and Management, Alagappa University, Karaikudi-630003, India
Narayanasamy Marimuthu Prabhu
Department of Animal Health and Management, Alagappa University, Karaikudi-630003, India
Gopal Selvakumar
Department of Microbiology, Science campus, Alagappa University, Karaikudi-630003, India
https://orcid.org/0000-0003-3087-4609

Corresponding Author(s) : Gopal Selvakumar

gselvakumar75@gmail.com

Asian Journal of Chemistry, Vol. 31 No. 7 (2019): Vol 31 Issue 7

Abstract

Antibiotic resistance is a huge problem that stays to challenge the healthcare sector in the world. The spread of multi drug resistant (MDR) bacteria remains a widely an unsolved problem. In the present study, antibacterial efficacy of cyanobacterium, Oxynema thaianum mediated silver nanoparticles (AgNPs) against extended-spectrum β-lactamases (ESBL) producing MDR strains were investigated. The UV-visible spectrum of biosynthesized AgNPs exhibited a characteristic SPR peak at 430 nm. The FT-IR, XRD, FESEM, EDAX and HRTEM analysis results confirms that the synthesized AgNPs were crystalline nature and spherical to oval in shape with size of 8-50 nm. The AgNPs synthesized from O. thaianum demonstrates high inhibitory activity in disc diffusion, MIC and MBC assays against MDR Escherichia coli and Klebsiella pneumoniae. Moreover, The LC50 value of AgNPs against A549 cells and Artemia nauplii were recorded as 7.2 and 53.33 μg mL-1, respectively. Therefore the study concluded that O. thaianum mediated AgNPs can be used as an alternative biological agent to control ESBL producing MDR pathogens.

Keywords

Antibiotic resistance Cyanobacteria ESBL AgNPs Toxicity
Padmini, N., Rashiya, N., Sivakumar, N., Kannan, N. D., Manjuladevi, R., Rajasekar, P., … Selvakumar, G. (2019). Green Synthesis of Silver Nanoparticles from Oxynema thaianum ALU PBC5 and their in vitro and in vivo Activity Against ESBL Producing MDR Escherichia coli and Klebsiella pneumoniae. Asian Journal of Chemistry, 31(7), 1447–1453. https://doi.org/10.14233/ajchem.2019.21821
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