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Vol. 30 No. 6 (2018): Vol 30 Issue 6

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FTIR Spectroscopic Study of Heat-Induced β-Lactoglobulin Solution under Flow Field

https://doi.org/10.14233/ajchem.2018.21232
R.K. Sharma
Polymer and Tissue Science Laboratory, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan; FPRC Laboratory, Department of Civil Engineering, Faculty of Engineering, Cape Peninsula University of Technology, Cape Town, South Africa
K. Furusawa
Polymer and Tissue Science Laboratory, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
A. Fukui
Polymer and Tissue Science Laboratory, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
N. Sasaki
Polymer and Tissue Science Laboratory, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
I. Masalova
FPRC Laboratory, Department of Civil Engineering, Faculty of Engineering, Cape Peninsula University of Technology, Cape Town, South Africa

Corresponding Author(s) : R.K. Sharma

rajechem@gmail.com

Asian Journal of Chemistry, Vol. 30 No. 6 (2018): Vol 30 Issue 6

Abstract

Effect of stirring on the secondary structure of amyloid fibrillogenesis of β-lactoglobulin (βLG) at pH 2 and at pH 7 with and without glucose was studied. Fibrillogenesis at pH 2 was carried out by heating the 4w % β-lactoglobulin and at pH 7 by heating the 0.30 mM β-lactoglobulin in 0.1 M, pH 7 sodium phosphate buffer solution with and without glucose (37.5 mM) for 24 h under stirring (250 and 474 rpm) conditions. For control samples, β-lactoglobulin solutions were incubated under unstirred condition at pH 2 and pH 7. The secondary structure of the amyloid fibrils which corresponds the β-sheet structure was studied by using Fourier transform infrared (FTIR) spectroscopy revealed that the stirring does not affect the secondary structure of the β-lactoglobulin fibrils at pH 2 as well as pH 7 with and without glucose.

Keywords

β-Lactoglobulin Fibrillogenesis Glucose FTIR
Sharma, R., Furusawa, K., Fukui, A., Sasaki, N., & Masalova, I. (2018). FTIR Spectroscopic Study of Heat-Induced β-Lactoglobulin Solution under Flow Field. Asian Journal of Chemistry, 30(6), 1321–1324. https://doi.org/10.14233/ajchem.2018.21232
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