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Effect of Fat Extraction Methods on Fatty Acid and Infrared Profiles of Chicken Fat Using GC-MS and FTIR
Corresponding Author(s) : Aminullah
Asian Journal of Chemistry,
Vol. 30 No. 6 (2018): Vol 30 Issue 6
Abstract
Chicken fat was obtained from rendering and chicken meat processing. The objective of present work was to study fatty acid and infrared characteristics of chicken fat based on several extraction methods. The sample preparation using Soxhlet, Folch, Bligh & Dyer and Hara & Radin methods, analysis of fatty acid using GC-MS and infrared characteristics of chicken fats using FTIR. Analysis of fatty acid showed that both SFA and USFA in Folch were not significantly different with those in Soxhlet and Hara and Radin at 5 % level, however significantly different with those in Bligh and Dyer. In addition, PCA showed fatty acids in Folch can be separated from others. FTIR spectra in general showed no significant differences in C-H stretching at 2922-2852 cm-1, C=O stretching at 1744-1376 cm-1 and the fingerprint absorption at 1000-900 cm-1. In addition, there were two peaks that indicate C-O-C group with force constant of C-O was about 1940 Nm-1.
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- R.I. Depkes, Komposisi Zat Gizi Pangan Indonesia, Jakarta (1995) (in Indonesian).
- A. Triyantini, I.A.K. Bintang and T.D. Antawidjaja, J. Ilmu Ternak Vet., 2, 157 (1997).
- C. Janusz, GC/MS Analysis for Unsaturated Fat Content in Animal Feed, Nafag Company, Switzerland (2003).
- I. Jaswir, M.E.S. Mirghani, T.H. Hassan and M.Z.M. Said, J. Oleo Sci., 52, 633 (2003); https://doi.org/10.5650/jos.52.633.
- F. David and P. Sandra, Column Selection For Analysis of Fatty Acid Methyl Esters, Research Institute For Chromatography Agilent Technology, USA (2005).
- S. Hermanto, A. Muawanah and R. Harahap, J. Kim. Valensi, 1, 102 (2008).
- A.F. Macedo, F.C. Taylor, J.P. Casas, A. Adler, D. Prieto-Merino and S. Ebrahim, BMC Med., 12, 51 (2014); https://doi.org/10.1186/1741-7015-12-51.
- S. Habeck, B. Mitchell and D. Sullivan, “Comparison of Fat Extraction Methods For Analysis of Meat”, 2013, Covance Laboratories Inc., USA.
- AOAC, “Official Method of Analysis of Association of Official Analytical Chemist”, 1995, 14th Ed: AOAC inc.
- J. Folch, M. Lees and S.G.H. Sloane, J. Biol. Chem., 226, 497 (1957).
- E.G. Bligh and W.J. Dyer, J. Biochem. Physiol., 37, 911 (1959).
- A. Hara and N.S. Radin, J. Anal. Biochem., 90, 420 (1978); https://doi.org/10.1016/0003-2697(78)90046-5.
- L. Metcalfe and A. Schmitz, Anal. Chem., 33, 363 (1961); https://doi.org/10.1021/ac60171a016.
- A. Rohman and K. Triyana, Int. Food Res. J., 19, 475 (2012).
- L. Hilda, Tazkir, 9, 1 (2014).
- A. Guntarti, S. Martono, A. Yuswanto and A. Rohman, Asian J. Biochem., 10, 165 (2016); https://doi.org/10.3923/ajb.2015.165.172.
- W. Smink, W.J. Gerrits, R. Hovenier, M.J. Geelen, M.W. Verstegen and A.C. Beynen, Poult Sci., 89, 2432 (2010); https://doi.org/10.3382/ps.2010-00665.
- J. Sedman, F.R. Van de Voort and A.A. Ismail, Application of Fourier Transform Infrared Spectroscopy in New Techniques and Application in Lipid Analysis, AOCS Press, Champaign, Illinois (1995).
- Y.B. Che Man and M.E.S. Mirghani, J. Am. Oil Chem. Soc., 78, 753 (2001); https://doi.org/10.1007/s11746-001-0338-4.
- R.M. Silverstein, G.C. Bassler and T.C. Morill, Spectrometric Identification of Organic Compounds, John Wiley & Sons, USA, edn 4 (1981).
- R.L. Brooks, The Fundamentals of Atomic and Molecular Physics, Springer, New York (2013).
References
R.I. Depkes, Komposisi Zat Gizi Pangan Indonesia, Jakarta (1995) (in Indonesian).
A. Triyantini, I.A.K. Bintang and T.D. Antawidjaja, J. Ilmu Ternak Vet., 2, 157 (1997).
C. Janusz, GC/MS Analysis for Unsaturated Fat Content in Animal Feed, Nafag Company, Switzerland (2003).
I. Jaswir, M.E.S. Mirghani, T.H. Hassan and M.Z.M. Said, J. Oleo Sci., 52, 633 (2003); https://doi.org/10.5650/jos.52.633.
F. David and P. Sandra, Column Selection For Analysis of Fatty Acid Methyl Esters, Research Institute For Chromatography Agilent Technology, USA (2005).
S. Hermanto, A. Muawanah and R. Harahap, J. Kim. Valensi, 1, 102 (2008).
A.F. Macedo, F.C. Taylor, J.P. Casas, A. Adler, D. Prieto-Merino and S. Ebrahim, BMC Med., 12, 51 (2014); https://doi.org/10.1186/1741-7015-12-51.
S. Habeck, B. Mitchell and D. Sullivan, “Comparison of Fat Extraction Methods For Analysis of Meat”, 2013, Covance Laboratories Inc., USA.
AOAC, “Official Method of Analysis of Association of Official Analytical Chemist”, 1995, 14th Ed: AOAC inc.
J. Folch, M. Lees and S.G.H. Sloane, J. Biol. Chem., 226, 497 (1957).
E.G. Bligh and W.J. Dyer, J. Biochem. Physiol., 37, 911 (1959).
A. Hara and N.S. Radin, J. Anal. Biochem., 90, 420 (1978); https://doi.org/10.1016/0003-2697(78)90046-5.
L. Metcalfe and A. Schmitz, Anal. Chem., 33, 363 (1961); https://doi.org/10.1021/ac60171a016.
A. Rohman and K. Triyana, Int. Food Res. J., 19, 475 (2012).
L. Hilda, Tazkir, 9, 1 (2014).
A. Guntarti, S. Martono, A. Yuswanto and A. Rohman, Asian J. Biochem., 10, 165 (2016); https://doi.org/10.3923/ajb.2015.165.172.
W. Smink, W.J. Gerrits, R. Hovenier, M.J. Geelen, M.W. Verstegen and A.C. Beynen, Poult Sci., 89, 2432 (2010); https://doi.org/10.3382/ps.2010-00665.
J. Sedman, F.R. Van de Voort and A.A. Ismail, Application of Fourier Transform Infrared Spectroscopy in New Techniques and Application in Lipid Analysis, AOCS Press, Champaign, Illinois (1995).
Y.B. Che Man and M.E.S. Mirghani, J. Am. Oil Chem. Soc., 78, 753 (2001); https://doi.org/10.1007/s11746-001-0338-4.
R.M. Silverstein, G.C. Bassler and T.C. Morill, Spectrometric Identification of Organic Compounds, John Wiley & Sons, USA, edn 4 (1981).
R.L. Brooks, The Fundamentals of Atomic and Molecular Physics, Springer, New York (2013).