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Vol. 30 No. 11 (2018): Vol 30 Issue 11

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Interaction of Cephalexin Monohydrate and Diclofenac Sodium with Human Erythrocyte Membrane: Adsorption Study and Equilibrium Isotherm Analysis

https://doi.org/10.14233/ajchem.2018.21532
Saja S. Al-Taweel
Department of Chemistry, College of Science, University of Al-Qadisiyah, Al-Diwaniyah, Qadisiyyah Province, Iraq
Saadoon A. Isa
Al-Hikma Foundation, Canada
Ramzi R. Al-Ani
Department of Chemistry, College of Science, University of Al-Mustansiriyah, Baghdad, Iraq

Corresponding Author(s) : Saja S. Al-Taweel

saja.Al-Taweel@qu.edu.iq

Asian Journal of Chemistry, Vol. 30 No. 11 (2018): Vol 30 Issue 11

Abstract

To elucidate the mechanism of interaction of biological surfaces with drugs, in vitro study of interaction of erythrocyte membrane from healthy individuals with two type of drugs was performed at 37.5 ºC and at physiological pH 7.4. Cephalexin monohydrate and diclofenac sodium were chosen for this purpose. The blood samples were collected from healthy volunteers (21 males and 19 females), aged 20 to 40 years (mean age 28 ± 6 years). Hypotonic solution was used to isolate the erythrocyte membrane from red blood cells. The amount of cephalexin·H2O or diclofenac sodium binding to erythrocyte membrane was significantly increased (p < 0.01) with increasing drug concentration and there was no significant difference (p > 0.05) in amount of drugs uptake by erythrocyte membrane of male or female. There was no significant difference (p > 0.05) between the amount of cephalexin·H2O binding to erythrocyte membrane and that for diclofenac sodium. The results indicated the applicability of Langmuir and Freundlich isotherms of drugs binding to erythrocyte membrane. The models that fit the experimental data assume the presence of multiple binding sites on the surface of erythrocyte membrane. The binding data and Scatchard plot suggested the existence of non-identical independent sites, in human erythrocyte membrane, interacting with cephalexin·H2O and having different affinities.

Keywords

Erythrocyte membrane Interaction Cephalexin monohydrate Diclofenac sodium Adsorption Isotherms
Al-Taweel, S. S., Isa, S. A., & Al-Ani, R. R. (2018). Interaction of Cephalexin Monohydrate and Diclofenac Sodium with Human Erythrocyte Membrane: Adsorption Study and Equilibrium Isotherm Analysis. Asian Journal of Chemistry, 30(11), 2539–2543. https://doi.org/10.14233/ajchem.2018.21532
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