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Vol. 35 No. 12 (2023): Vol 35 Issue 12, 2023

Copyright (c) 2023 Y. Manogaran, S. Karupiah, V. Balakrishnan, N. K. Fuloria, S. FULORIA

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Synthesis and Evaluation of Anticancer Potential of Novel Benzopyran Derivatives Using MTT and in vitro Scratch Assays

https://doi.org/10.14233/ajchem.2023.30282
Y. Manogaran
Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia
https://orcid.org/0009-0008-9778-0803
S. Karupiah
Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia
https://orcid.org/0000-0003-2298-7507
V. Balakrishnan
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia
https://orcid.org/0000-0002-4874-600X
N. K. Fuloria
Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia
https://orcid.org/0000-0002-8970-5212
S. Fuloria
Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia
https://orcid.org/0000-0002-5381-5655

Corresponding Author(s) : S. Fuloria

shivkanya_fuloria@aimst.edu.my

Asian Journal of Chemistry, Vol. 35 No. 12 (2023): Vol 35 Issue 12, 2023

Abstract

Evidence over benzopyrans and azomethines high anticancer potential, intended present study to carry out the synthesis, characterization and anticancer activity of some new benzopyrans derivatives (NBD). Study involved synthesis of N'-(substituted benzylidene)-2-(2-oxo-2H-benzopyran-4-yloxy)acetohydrazide (2a-d) by hydrazination of substituted benzopyran ester (1). The structures of all the synthesized benzopyran derivatives (NBD 2a-d) were elucidated based on the IR, 1H NMR, 13C NMR and mass spectral data. The NBD 2a-d were further investigated for their cell viability (toxicity analysis) against HEK-293 and anticancer potential against MCF-7 cancer cell lines using MTT assay and in vitro scratch assay. Present study revealed that NBD 2a-d structures were in agreement with their IR, NMR and mass spectrometric characterization data. The cell viability study, MTT and in vitro scratch assay of NBD 2a-d, revealed compound 2d to exhibit highest anticancer activity and safety when compared with standard (irinotecan). Present study concludes NBD 2d to possess high anticancer potential and proven to be safer when compared with irinotecan, however further studies are needed to establish its clinical significance.

Keywords

Benzopyran Imino analogues Anticancer activity Cytotoxicity
Manogaran, Y., Karupiah, S., Balakrishnan, V., Fuloria, N. K., & Fuloria, S. (2023). Synthesis and Evaluation of Anticancer Potential of Novel Benzopyran Derivatives Using MTT and in vitro Scratch Assays. Asian Journal of Chemistry, 35(12), 2948–2954. https://doi.org/10.14233/ajchem.2023.30282
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