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Vol. 27 No. 12 (2015): Vol 27 Issue 12

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Ultrasound Wave Assisted-Matrix Solid Phase Extraction for Determination of Synthetic Plant Growth Regulators Residues in Fruits and Vegetables by Ultra-High Performance Liquid Chromatography

https://doi.org/10.14233/ajchem.2015.19138
Hongcheng Liu
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China
Tao Lin
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China
Yanhong Zou
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China
Jinliang Shao
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China
Dongshun Yang
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China
Qiwan Li
Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, P.R. China

Corresponding Author(s) : Qiwan Li

cmliu_0@sina.com

Asian Journal of Chemistry, Vol. 27 No. 12 (2015): Vol 27 Issue 12

Abstract

An effective method using ultra-high performance liquid chromatography (UHPLC) was developed and optimized to obtain a complete separation of four representative synthetic plant growth regulators e.g., [2,4-dichlorophenoxyacetic acid (2,4-D), thidiazuron, forchlorfenuron and paclobutrazol] in fruits and vegetables. Extraction was performed with acetonitrile containing 0.5 % (v/v) acetic acid + 5 g sodium chloride (pH 4) under 3 min with an ultrasound wave assisted bath (40 kHz, 50 W), then 2 mL organic extraction and 1 g sorbents mixed, purified, analyzed by UHPLC and HPLC. The performance of chromatography was assessed by UHPLC and HPLC. Good recoveries and relative standard deviation were found for all analytes in fruits and vegetables.

Keywords

Synthetic plant growth regulators Ultrasound wave assisted extraction Matrix solid phase extraction UHPLC
Liu, H., Lin, T., Zou, Y., Shao, J., Yang, D., & Li, Q. (2015). Ultrasound Wave Assisted-Matrix Solid Phase Extraction for Determination of Synthetic Plant Growth Regulators Residues in Fruits and Vegetables by Ultra-High Performance Liquid Chromatography. Asian Journal of Chemistry, 27(12), 4387–4391. https://doi.org/10.14233/ajchem.2015.19138
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