Copyright (c) 2014 a Saosoong, Chalerm Ruangviriyachai*
This work is licensed under a Creative Commons Attribution 4.0 International License.
Antioxidant and Antimicrobial Activities of Methanolic Extract from Jatropha curcas Linn. Fruit
Corresponding Author(s) : a Saosoong
Asian Journal of Chemistry,
Vol 26 No Supplementary Issue
Abstract
The present study was designed to evaluate the antioxidant activity and also antimicrobial activity with total phenolic content of the crude methanolic extract from J. curcas Linn. fruit. The dried samples were extracted by 60 % (v/v) methanol to obtain the crude methanolic extract. The total phenolic content of the crude methanolic extract which was analyzed by Folin-Ciocalteu method was 7.04 ± 0.10 mg/g as GAE. The evaluation of antioxidant activity of the crude methanolic extract was performed by using two methods including 2,2-diphenyl-1-picryhydrazyl and Phen assay. The results showed that the antioxidant activity of this extract was 270.98 ± 0.59 μmol Fe/g of extract for Phen assay and the extract gave IC50 at 14.09 ± 0.05 mg/mL for 2,2-diphenyl-1-picryhydrazyl assay. Additionally, as the antimicrobial activity evaluation, this extract exhibited the potencies of antimicrobial activity against P. putida, P. syringae pv. sesami, X. campestris, X. campestris pv. glycines, X. campestris pv. vesicatoria and R. solanacearum with the presence of inhibition zone in the range of 8.0 ± 0.0 to 13.7 ± 0.6 mm and MIC value at 214.29 ± 0.00 μg/mL. All test methods showed that the crude methanolic extract from J. curcas Linn. fruit had a higher total phenolic content and antioxidant activity than J. curcas seed oil in previous work, while the antimicrobial activity was moderated. Therefore, these results suggested that J. curcas Linn. fruit has highly potential as effective natural bioactive sources.
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References
R.M. Jingura, D. Musademba and R. Matengaifa, Int. J. Eng. Sci. Technol., 2, 115 (2010).
F. Cai, C. OuYang, P. Duan, S. Gao, Y. Xu and F. Chen, J. Mol. Catal. B, 77, 59 (2012).
R. Fu, Y. Zhang, Y. Guo, F. Liu and F. Chen, Ind. Crops Prod., 58, 265 (2014).
X. Liu, L. Li, W. Li, D. Lu, F. Chen and J. Li, Ind. Crops Prod., 47, 29 (2013).
O.O. Igbinosa, I.H. Igbinosa, V.N. Chigor, O.E. Uzunuigbe, S.O. Oyedemi, E.E. Odjadjare, A.I. Okoh and E.O. Igbinosa, Int. J. Mol. Sci., 12, 2958 (2011).
L. Yao, C. Han, G. Chen, X. Song, Y. Chang and W. Zang, Fitoterapia, 83, 1318 (2012).
X.Q. Zhang, F. Li, Z.G. Zhao, X.L. Liu, Y.X. Tang and M.K. Wang, Phytochem. Lett., 5, 721 (2012).
N.A. Ehsan Oskoueian, J. Med. Plants Res., 5, 49 (2011).
W. Tongpoothorn, S. Chanthai, M. Sriuttha, C. Ruangviriyachai and K. Saosang, Ind. Crops Prod., 36, 437 (2012).
S. Bounatirou, S. Smiti, M.G. Miguel, L. Faleiro, M.N. Rejeb, M. Neffati, M.M. Costa, A.C. Figueiredo, J.G. Barroso and L.G. Pedro, Food Chem., 105, 146 (2007).
N. Gourine, M. Yousfi, I. Bombarda, B. Nadjemi, P. Stocker and E.M. Gaydou, Ind. Crops Prod., 31, 203 (2010).
R. Bruni, D. Rossi, M. Muzzoli, C. Romagnoli, G. Paganetto, E. Besco, F. Choquecillo, K. Peralta, W.S. Lora and G. Sacchetti, Fitoterapia, 77, 538 (2006).
A. Szydlowska-Czerniak, C. Dianoczki, K. Recseg, G. Karlovits and E. Szlyk, Talanta, 76, 899 (2008).
S. Chan-Eam, S. Teerasong, K. Damwan, D. Nacapricha and R. Chaisuksant, Talanta, 84, 1350 (2011).
P. Ganesan, C.S. Kumar and N. Bhaskar, Bioresour. Technol., 99, 2717 (2008).
S. Nithiyanantham, P. Siddhuraju and G. Francis, Ind. Crops Prod., 43, 261 (2013).
M. Gulluce, F. Sahin, M. Sokmen, H. Ozer, D. Daferera, A. Sokmen, M. Polissiou, A. Adiguzel and H. Ozkan, Food Chem., 103, 1449 (2007).
S. Hogg, Essential Microbiology, John Wiley & Sons, England, pp. 353-368 (2005).
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F. Oke, B. Aslim, S. Ozturk and S. Altundag, Food Chem., 112, 874 (2009).
N. Erkan, G. Ayranci and E. Ayranci, Food Chem., 110, 76 (2008).
L. Scorzoni, T. Benaducci, A.M.F. Almeida, D.H.S. Silva, V.S. Bolzani and M.J.S. Mendes-Giannini, Rev. Cienc. Farm. Basica Appl., 28, 25 (2007).